The gonad growth of Anguilla bicolor bicolor induced by hormone combination of HCG, MT, E2, and antidopamine

ABSTRACT   The aim of the study was to stimulate eel gonad growth by intramuscularly injection with a hormonal combination of human chorionic gonadotropin (hCG), methyltestosterone (T), estradiol (E2) and antidopamine (A) meanwhile 0.9% NaCl solution was used as control. Estradiol concentration in t...

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Main Authors: Abdul Zahri, Agus Oman Sudrajat, Muhammad Zairin Junior
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2015-10-01
Series:Jurnal Akuakultur Indonesia
Online Access:https://journal.ipb.ac.id/index.php/jai/article/view/10329
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author Abdul Zahri
Agus Oman Sudrajat
Muhammad Zairin Junior
author_facet Abdul Zahri
Agus Oman Sudrajat
Muhammad Zairin Junior
author_sort Abdul Zahri
collection DOAJ
description ABSTRACT   The aim of the study was to stimulate eel gonad growth by intramuscularly injection with a hormonal combination of human chorionic gonadotropin (hCG), methyltestosterone (T), estradiol (E2) and antidopamine (A) meanwhile 0.9% NaCl solution was used as control. Estradiol concentration in the blood serum were measured by enzyme-linked immunosorbent assay (ELISA). Stimulation with hCG 20 IU/kg in combination with MT 3 mg/kg and 10 μg/kg antidopamine (hTA treatment) very effective for increased the growth of gonads with indicators gonadosomatic index (GSI) of 4.80%, hepatosomatic index 1.01% and concentration of E2 2.49±0.67 ng/mL were significantly different (P<0.05) than others treatment after ten weeks of application.   Key words: hormone, gonad growth, maturation, Indonesian short finned eel     ABSTRAK   Penelitian ini bertujuan untuk mengetahui pertumbuhan gonad yang distimulasi oleh kombinasi human chorionic gonadotropin (hCG), metiltestosteron (MT), estradiol (E2) dan antidopamin (A). Ikan sidat disuntik dengan hormon dan 0,9% NaCl sebagai kontrol secara intramuskular. Pengukuran konsentrasi hormon E2 dalam serum darah dengan enzyme-linked immunosorbent assay (ELISA). Stimulasi dengan hCG 20 IU/kg dikombinasi dengan MT 3 mg/kg dan antidopamin 10 μg/kg (perlakuan hTA) sangat efektif untuk feminisasi dan meningkatkan pertumbuhan gonad dengan indikator indeks gonadosomatik (GSI) 4,80%, indeks hepatosomatik 1,01% dan konsentrasi E2 2,49±0,67 ng/mL, secara signifikan berbeda pada taraf P<0,05 dibandingkan perlakuan lain setelah sepuluh minggu aplikasi.   Kata kunci: hormon, pertumbuhan gonad, maturasi, sidat
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spelling doaj-art-79517dcdf53642bbbc624294ab7d6df82025-01-24T17:58:49ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-10-0114110.19027/jai.14.69-788508The gonad growth of Anguilla bicolor bicolor induced by hormone combination of HCG, MT, E2, and antidopamineAbdul Zahri0Agus Oman Sudrajat1Muhammad Zairin Junior2Departemen Budidaya Perairan Institut Pertanian BogorDepartemen Budidaya Perairan Institut Pertanian BogorDepartemen Budidaya Perairan Institut Pertanian BogorABSTRACT   The aim of the study was to stimulate eel gonad growth by intramuscularly injection with a hormonal combination of human chorionic gonadotropin (hCG), methyltestosterone (T), estradiol (E2) and antidopamine (A) meanwhile 0.9% NaCl solution was used as control. Estradiol concentration in the blood serum were measured by enzyme-linked immunosorbent assay (ELISA). Stimulation with hCG 20 IU/kg in combination with MT 3 mg/kg and 10 μg/kg antidopamine (hTA treatment) very effective for increased the growth of gonads with indicators gonadosomatic index (GSI) of 4.80%, hepatosomatic index 1.01% and concentration of E2 2.49±0.67 ng/mL were significantly different (P<0.05) than others treatment after ten weeks of application.   Key words: hormone, gonad growth, maturation, Indonesian short finned eel     ABSTRAK   Penelitian ini bertujuan untuk mengetahui pertumbuhan gonad yang distimulasi oleh kombinasi human chorionic gonadotropin (hCG), metiltestosteron (MT), estradiol (E2) dan antidopamin (A). Ikan sidat disuntik dengan hormon dan 0,9% NaCl sebagai kontrol secara intramuskular. Pengukuran konsentrasi hormon E2 dalam serum darah dengan enzyme-linked immunosorbent assay (ELISA). Stimulasi dengan hCG 20 IU/kg dikombinasi dengan MT 3 mg/kg dan antidopamin 10 μg/kg (perlakuan hTA) sangat efektif untuk feminisasi dan meningkatkan pertumbuhan gonad dengan indikator indeks gonadosomatik (GSI) 4,80%, indeks hepatosomatik 1,01% dan konsentrasi E2 2,49±0,67 ng/mL, secara signifikan berbeda pada taraf P<0,05 dibandingkan perlakuan lain setelah sepuluh minggu aplikasi.   Kata kunci: hormon, pertumbuhan gonad, maturasi, sidat https://journal.ipb.ac.id/index.php/jai/article/view/10329
spellingShingle Abdul Zahri
Agus Oman Sudrajat
Muhammad Zairin Junior
The gonad growth of Anguilla bicolor bicolor induced by hormone combination of HCG, MT, E2, and antidopamine
Jurnal Akuakultur Indonesia
title The gonad growth of Anguilla bicolor bicolor induced by hormone combination of HCG, MT, E2, and antidopamine
title_full The gonad growth of Anguilla bicolor bicolor induced by hormone combination of HCG, MT, E2, and antidopamine
title_fullStr The gonad growth of Anguilla bicolor bicolor induced by hormone combination of HCG, MT, E2, and antidopamine
title_full_unstemmed The gonad growth of Anguilla bicolor bicolor induced by hormone combination of HCG, MT, E2, and antidopamine
title_short The gonad growth of Anguilla bicolor bicolor induced by hormone combination of HCG, MT, E2, and antidopamine
title_sort gonad growth of anguilla bicolor bicolor induced by hormone combination of hcg mt e2 and antidopamine
url https://journal.ipb.ac.id/index.php/jai/article/view/10329
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