In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil
Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identi...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2014/102968 |
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| author | Ryan M. Williams Amanda R. Kulick Srilakshmi Yedlapalli Louisa Battistella Cyrus J. Hajiran Letha J. Sooter |
| author_facet | Ryan M. Williams Amanda R. Kulick Srilakshmi Yedlapalli Louisa Battistella Cyrus J. Hajiran Letha J. Sooter |
| author_sort | Ryan M. Williams |
| collection | DOAJ |
| description | Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (Kd=9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device. |
| format | Article |
| id | doaj-art-794189a553e84456b91d516cdd7e19fe |
| institution | Kabale University |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-794189a553e84456b91d516cdd7e19fe2025-02-03T06:00:43ZengWileyJournal of Nucleic Acids2090-02012090-021X2014-01-01201410.1155/2014/102968102968In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for BromacilRyan M. Williams0Amanda R. Kulick1Srilakshmi Yedlapalli2Louisa Battistella3Cyrus J. Hajiran4Letha J. Sooter5Department of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USADepartment of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USADepartment of Chemistry, West Virginia University, 217 Clark Hall, Morgantown, WV 26506, USADepartment of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USADepartment of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USADepartment of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USABromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (Kd=9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.http://dx.doi.org/10.1155/2014/102968 |
| spellingShingle | Ryan M. Williams Amanda R. Kulick Srilakshmi Yedlapalli Louisa Battistella Cyrus J. Hajiran Letha J. Sooter In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil Journal of Nucleic Acids |
| title | In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil |
| title_full | In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil |
| title_fullStr | In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil |
| title_full_unstemmed | In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil |
| title_short | In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil |
| title_sort | in vitro selection of a single stranded dna molecular recognition element specific for bromacil |
| url | http://dx.doi.org/10.1155/2014/102968 |
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