Nutritional, phenolic and in vitro digestion characteristics of dried grape pomace processed with different micronization/air-classification protocols

Grape pomace requires drying to prevent spoilage and ensure year-round use. This study evaluates the nutrient composition, in vitro digestibility, gas production, and phenolic content of dried grape pomace processed with different micronization and air-classification techniques. Dried grape pomace w...

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Main Authors: Aristide Maggiolino, Roberta Greco, Federica Blando, Eleni Tsiplakou, Elisabetta Casalino, Pasquale De Palo
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Italian Journal of Animal Science
Subjects:
Online Access:http://dx.doi.org/10.1080/1828051X.2025.2454922
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author Aristide Maggiolino
Roberta Greco
Federica Blando
Eleni Tsiplakou
Elisabetta Casalino
Pasquale De Palo
author_facet Aristide Maggiolino
Roberta Greco
Federica Blando
Eleni Tsiplakou
Elisabetta Casalino
Pasquale De Palo
author_sort Aristide Maggiolino
collection DOAJ
description Grape pomace requires drying to prevent spoilage and ensure year-round use. This study evaluates the nutrient composition, in vitro digestibility, gas production, and phenolic content of dried grape pomace processed with different micronization and air-classification techniques. Dried grape pomace was micronized at frequencies of 85 Hz (LH) and 170 Hz (HH), then air-classified at pressures of 200 psi (LP) and 240 psi (HP) into coarse (G), middle fine (F1), and fine (F2) fractions. The four experimental groups were: low Hz low PSI (LH-LP), low Hz high PSI (LH-HP), high Hz low PSI (HH-LP), and high Hz high PSI (HH-HP). The HH-HP treatment resulted in the highest crude protein (p < 0.01) in the F1 fraction, and the LH-LP treatment showed the highest ether extract content (p < 0.01) in the F2 fraction. Additionally, the HH-HP method produced the highest crude fibre (p < 0.01) in the G fraction. Rutin was more concentrated in the G fraction and lesser in F2 (p < 0.05). The highest phenolic concentration was recorded in the F1 fraction under high micronization (p < 0.05), while G fraction had the lowest Folin values (p < 0.05). These findings suggest that fine fractions enriched in polyphenols and antioxidant activity, offer promising benefits for animal feed applications by potentially improving feed functionality and health-promoting properties. In conclusion, micronization and air-classification improved the protein content of finer grape pomace fractions but slightly reduced protein digestibility, likely due to interactions with phenolics or increased fibre. Overall, the optimised processing techniques show potential to make grape pomace a sustainable and functional feed ingredient for ruminants.
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institution Kabale University
issn 1594-4077
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language English
publishDate 2025-12-01
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series Italian Journal of Animal Science
spelling doaj-art-7937c503e862449e91dc5b3c7d5907b32025-01-27T11:59:10ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2025-12-0124139341010.1080/1828051X.2025.24549222454922Nutritional, phenolic and in vitro digestion characteristics of dried grape pomace processed with different micronization/air-classification protocolsAristide Maggiolino0Roberta Greco1Federica Blando2Eleni Tsiplakou3Elisabetta Casalino4Pasquale De Palo5Dipartimento di Medicina Veterinaria, Università degli studi di Bari A. MoroDipartimento di Scienze del Suolo, della pianta e degli Alimenti, Università degli Studi di Bari A. MoroIstituto di Scienze delle Produzioni Alimantari, Consiglio Nazionale delle RicercheNutritional Physiology and Feeding, Department of Animal Science, School of Animal Biosciences, Agricultural University of AthensDipartimento di Medicina Veterinaria, Università degli studi di Bari A. MoroDipartimento di Medicina Veterinaria, Università degli studi di Bari A. MoroGrape pomace requires drying to prevent spoilage and ensure year-round use. This study evaluates the nutrient composition, in vitro digestibility, gas production, and phenolic content of dried grape pomace processed with different micronization and air-classification techniques. Dried grape pomace was micronized at frequencies of 85 Hz (LH) and 170 Hz (HH), then air-classified at pressures of 200 psi (LP) and 240 psi (HP) into coarse (G), middle fine (F1), and fine (F2) fractions. The four experimental groups were: low Hz low PSI (LH-LP), low Hz high PSI (LH-HP), high Hz low PSI (HH-LP), and high Hz high PSI (HH-HP). The HH-HP treatment resulted in the highest crude protein (p < 0.01) in the F1 fraction, and the LH-LP treatment showed the highest ether extract content (p < 0.01) in the F2 fraction. Additionally, the HH-HP method produced the highest crude fibre (p < 0.01) in the G fraction. Rutin was more concentrated in the G fraction and lesser in F2 (p < 0.05). The highest phenolic concentration was recorded in the F1 fraction under high micronization (p < 0.05), while G fraction had the lowest Folin values (p < 0.05). These findings suggest that fine fractions enriched in polyphenols and antioxidant activity, offer promising benefits for animal feed applications by potentially improving feed functionality and health-promoting properties. In conclusion, micronization and air-classification improved the protein content of finer grape pomace fractions but slightly reduced protein digestibility, likely due to interactions with phenolics or increased fibre. Overall, the optimised processing techniques show potential to make grape pomace a sustainable and functional feed ingredient for ruminants.http://dx.doi.org/10.1080/1828051X.2025.2454922grape pomacemicronizationair-classificationpolyphenolsgas-production
spellingShingle Aristide Maggiolino
Roberta Greco
Federica Blando
Eleni Tsiplakou
Elisabetta Casalino
Pasquale De Palo
Nutritional, phenolic and in vitro digestion characteristics of dried grape pomace processed with different micronization/air-classification protocols
Italian Journal of Animal Science
grape pomace
micronization
air-classification
polyphenols
gas-production
title Nutritional, phenolic and in vitro digestion characteristics of dried grape pomace processed with different micronization/air-classification protocols
title_full Nutritional, phenolic and in vitro digestion characteristics of dried grape pomace processed with different micronization/air-classification protocols
title_fullStr Nutritional, phenolic and in vitro digestion characteristics of dried grape pomace processed with different micronization/air-classification protocols
title_full_unstemmed Nutritional, phenolic and in vitro digestion characteristics of dried grape pomace processed with different micronization/air-classification protocols
title_short Nutritional, phenolic and in vitro digestion characteristics of dried grape pomace processed with different micronization/air-classification protocols
title_sort nutritional phenolic and in vitro digestion characteristics of dried grape pomace processed with different micronization air classification protocols
topic grape pomace
micronization
air-classification
polyphenols
gas-production
url http://dx.doi.org/10.1080/1828051X.2025.2454922
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