A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans
Candida auris occupies similar niches in various infections as Pseudomonas aeruginosa; however, the details of their interspecies communication remain largely unknown. To gain deeper insights into this relationship, phenotypic and transcriptomic analyses were conducted in the presence of the primary...
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Elsevier
2025-06-01
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| Series: | Biofilm |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207525000073 |
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| author | Fruzsina Kovács Ágnes Jakab Noémi Balla Zoltán Tóth Dávid Balázsi Lajos Forgács Andrea Harmath Aliz Bozó Ágota Ragyák László Majoros Renátó Kovács |
| author_facet | Fruzsina Kovács Ágnes Jakab Noémi Balla Zoltán Tóth Dávid Balázsi Lajos Forgács Andrea Harmath Aliz Bozó Ágota Ragyák László Majoros Renátó Kovács |
| author_sort | Fruzsina Kovács |
| collection | DOAJ |
| description | Candida auris occupies similar niches in various infections as Pseudomonas aeruginosa; however, the details of their interspecies communication remain largely unknown. To gain deeper insights into this relationship, phenotypic and transcriptomic analyses were conducted in the presence of the primary P. aeruginosa quorum-sensing molecule, N-(3-oxododecanoyl)-l-homoserine lactone (HSL), against C. auris, with the results compared to those of C. albicans. Our findings indicate that HSL-induced effects are not specific to C. albicans; additionally, several characteristics are present in C. auris but not in C. albicans following HSL exposure. Significant HSL-induced reduction was observed in growth and adhesion of C. auris cells in time -and concentration-dependent way (p < 0.01-0.001). Moreover, HSL reduced intracellular iron and zinc levels (p < 0.05-0.001); furthermore, it modulated C. auris metabolism toward beta-oxidation, which may be associated with the observed reduction in in vivo virulence at lower HSL concentrations compared with C. albicans. RNA-sequencing transcriptome analysis of C. auris revealed 67 and 306 upregulated genes, as well as 111 and 168 downregulated genes, in response to 100 and 200-μM HSL, respectively. We identified 45 overlapping upregulated and 25 overlapping downregulated genes between the two HSL concentrations. Similar to other Candida-derived C12 compounds (e.g., farnesol), HSL reduces several C. auris survival strategies, which may significantly influence the nature of P. aeruginosa–C. auris co-habitation. In conclusion, the obtained findings on C. auris do not provide clear evidence that HSL mediated effects have any favourable consequences in terms of P. aeruginosa-C. auris co-colonisation and/or co-infections. |
| format | Article |
| id | doaj-art-7935d18620954bc59118d110eca5c748 |
| institution | Kabale University |
| issn | 2590-2075 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-7935d18620954bc59118d110eca5c7482025-02-06T05:12:45ZengElsevierBiofilm2590-20752025-06-019100259A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicansFruzsina Kovács0Ágnes Jakab1Noémi Balla2Zoltán Tóth3Dávid Balázsi4Lajos Forgács5Andrea Harmath6Aliz Bozó7Ágota Ragyák8László Majoros9Renátó Kovács10Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Hungary; Doctoral School of Pharmaceutical Sciences, University of Debrecen, 4032, Debrecen, HungaryDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, HungaryDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, Hungary; Doctoral School of Pharmaceutical Sciences, University of Debrecen, 4032, Debrecen, HungaryDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, HungaryDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, Hungary; Doctoral School of Pharmaceutical Sciences, University of Debrecen, 4032, Debrecen, HungaryDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, Hungary; Doctoral School of Pharmaceutical Sciences, University of Debrecen, 4032, Debrecen, HungaryDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, Hungary; Doctoral School of Pharmaceutical Sciences, University of Debrecen, 4032, Debrecen, HungaryDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, HungaryDepartment of Inorganic and Analytical Chemistry, Agilent Atomic Spectroscopy Partner Laboratory, University of Debrecen, Debrecen, HungaryDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, HungaryDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, Hungary; Corresponding author. Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98., 4032, Debrecen, Hungary.Candida auris occupies similar niches in various infections as Pseudomonas aeruginosa; however, the details of their interspecies communication remain largely unknown. To gain deeper insights into this relationship, phenotypic and transcriptomic analyses were conducted in the presence of the primary P. aeruginosa quorum-sensing molecule, N-(3-oxododecanoyl)-l-homoserine lactone (HSL), against C. auris, with the results compared to those of C. albicans. Our findings indicate that HSL-induced effects are not specific to C. albicans; additionally, several characteristics are present in C. auris but not in C. albicans following HSL exposure. Significant HSL-induced reduction was observed in growth and adhesion of C. auris cells in time -and concentration-dependent way (p < 0.01-0.001). Moreover, HSL reduced intracellular iron and zinc levels (p < 0.05-0.001); furthermore, it modulated C. auris metabolism toward beta-oxidation, which may be associated with the observed reduction in in vivo virulence at lower HSL concentrations compared with C. albicans. RNA-sequencing transcriptome analysis of C. auris revealed 67 and 306 upregulated genes, as well as 111 and 168 downregulated genes, in response to 100 and 200-μM HSL, respectively. We identified 45 overlapping upregulated and 25 overlapping downregulated genes between the two HSL concentrations. Similar to other Candida-derived C12 compounds (e.g., farnesol), HSL reduces several C. auris survival strategies, which may significantly influence the nature of P. aeruginosa–C. auris co-habitation. In conclusion, the obtained findings on C. auris do not provide clear evidence that HSL mediated effects have any favourable consequences in terms of P. aeruginosa-C. auris co-colonisation and/or co-infections.http://www.sciencedirect.com/science/article/pii/S2590207525000073Candida aurisPseudomonas aeruginosaIn vivoTranscriptomeHomoserine lactoneIron |
| spellingShingle | Fruzsina Kovács Ágnes Jakab Noémi Balla Zoltán Tóth Dávid Balázsi Lajos Forgács Andrea Harmath Aliz Bozó Ágota Ragyák László Majoros Renátó Kovács A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans Biofilm Candida auris Pseudomonas aeruginosa In vivo Transcriptome Homoserine lactone Iron |
| title | A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans |
| title_full | A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans |
| title_fullStr | A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans |
| title_full_unstemmed | A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans |
| title_short | A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans |
| title_sort | comprehensive analysis of the effect of quorum sensing molecule 3 oxo c12 homoserine lactone on candida auris and candida albicans |
| topic | Candida auris Pseudomonas aeruginosa In vivo Transcriptome Homoserine lactone Iron |
| url | http://www.sciencedirect.com/science/article/pii/S2590207525000073 |
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