Proteomic Analyses of the Vitreous Humour
The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine s...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/148039 |
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| _version_ | 1832545863171309568 |
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| author | Martina Angi Helen Kalirai Sarah E. Coupland Bertil E. Damato Francesco Semeraro Mario R. Romano |
| author_facet | Martina Angi Helen Kalirai Sarah E. Coupland Bertil E. Damato Francesco Semeraro Mario R. Romano |
| author_sort | Martina Angi |
| collection | DOAJ |
| description | The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine surgical procedures. Historically, many studies have investigated levels of individual proteins in VH from healthy and diseased eyes. In the last decade, proteomics analyses have been performed to characterise the proteome of the human VH and explore networks of functionally related proteins, providing insight into the aetiology of diabetic retinopathy and proliferative vitreoretinopathy. Recent proteomic studies on the VH from animal models of autoimmune uveitis have identified new signalling pathways associated to autoimmune triggers and intravitreal inflammation. This paper aims to guide biological scientists through the different proteomic techniques that have been used to analyse the VH and present future perspectives for the study of intravitreal inflammation using proteomic analyses. |
| format | Article |
| id | doaj-art-7933477e04ee4a398e0f9906f67c5faa |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7933477e04ee4a398e0f9906f67c5faa2025-02-03T07:24:29ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/148039148039Proteomic Analyses of the Vitreous HumourMartina Angi0Helen Kalirai1Sarah E. Coupland2Bertil E. Damato3Francesco Semeraro4Mario R. Romano5Liverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, Liverpool, UKLiverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, Liverpool, UKLiverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, Liverpool, UKLiverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, Liverpool, UKDepartment of Ophthalmology, Spedali Civili di Brescia, University of Brescia, Brescia, ItalyDepartment of Ophthalmology, Istituto Clinico Humanitas, Milan, ItalyThe human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine surgical procedures. Historically, many studies have investigated levels of individual proteins in VH from healthy and diseased eyes. In the last decade, proteomics analyses have been performed to characterise the proteome of the human VH and explore networks of functionally related proteins, providing insight into the aetiology of diabetic retinopathy and proliferative vitreoretinopathy. Recent proteomic studies on the VH from animal models of autoimmune uveitis have identified new signalling pathways associated to autoimmune triggers and intravitreal inflammation. This paper aims to guide biological scientists through the different proteomic techniques that have been used to analyse the VH and present future perspectives for the study of intravitreal inflammation using proteomic analyses.http://dx.doi.org/10.1155/2012/148039 |
| spellingShingle | Martina Angi Helen Kalirai Sarah E. Coupland Bertil E. Damato Francesco Semeraro Mario R. Romano Proteomic Analyses of the Vitreous Humour Mediators of Inflammation |
| title | Proteomic Analyses of the Vitreous Humour |
| title_full | Proteomic Analyses of the Vitreous Humour |
| title_fullStr | Proteomic Analyses of the Vitreous Humour |
| title_full_unstemmed | Proteomic Analyses of the Vitreous Humour |
| title_short | Proteomic Analyses of the Vitreous Humour |
| title_sort | proteomic analyses of the vitreous humour |
| url | http://dx.doi.org/10.1155/2012/148039 |
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