Unveiling microbial diversity in slightly and moderately magnesium deficient acidic soils
Abstract Magnesium (Mg) an essential plant nutrient is widespread deficient in the acidic soils of Nilgiris of Tamil nadu, India. The vegetable yield and quality is especially affected due to deficiency of nutrients like Mg. This study investigates soil characteristics and bacterial diversity in the...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-87943-3 |
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| author | Munmun Dash Subramaniam Thiyageshwari Duraisamy Selvi Haina K. V. Johnson Manikandan Ariyan Karuppusamy Rajan Rangasamy Anandham |
| author_facet | Munmun Dash Subramaniam Thiyageshwari Duraisamy Selvi Haina K. V. Johnson Manikandan Ariyan Karuppusamy Rajan Rangasamy Anandham |
| author_sort | Munmun Dash |
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| description | Abstract Magnesium (Mg) an essential plant nutrient is widespread deficient in the acidic soils of Nilgiris of Tamil nadu, India. The vegetable yield and quality is especially affected due to deficiency of nutrients like Mg. This study investigates soil characteristics and bacterial diversity in the Nilgiris district of Tamil Nadu, India, with respect to Mg deficiency. The soil samples were collected from different vegetable growing regions of the Nilgiris to assess soil physiocochemical parameters, soil enzymes and soil Mg status. 16S rRNA gene-based metagenomic analysis used to investigate the functional potential and structural diversity of the bacterial communities in high Mg and low Mg deficiency soil. Results indicated mildly acidic soils with a sandy loam texture and high organic carbon content. While nitrogen (N), phosphorus (P), and potassium (K) levels were adequate, Mg deficiency was consistent. Soil enzymes such as dehydrogenase, acid phosphatase, urease and aryl sulfatase, varied across the soil samples. Additionally, 16S rRNA gene-based metagenomics analysis revealed the bacterial diversity and functional pathways in soils with high and low Mg deficiency. Low Mg levels were associated with increased bacterial richness, dominated by Proteobacteria, Gemmatimonadetes, Actinobacteria, Bacteroidetes, and Acidobacteria. Functional pathways related to carbon metabolism, amino acid biosynthesis, and various metabolic processes were more abundant in low Mg deficient soils. This research highlights the significant influence of Mg levels on bacterial diversity and functional potentials in acidic soils, providing insights into soil management strategies in Mg-deficient regions. |
| format | Article |
| id | doaj-art-7901b0446b424c4486975d9b8eaee61f |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-7901b0446b424c4486975d9b8eaee61f2025-02-02T12:24:43ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-025-87943-3Unveiling microbial diversity in slightly and moderately magnesium deficient acidic soilsMunmun Dash0Subramaniam Thiyageshwari1Duraisamy Selvi2Haina K. V. Johnson3Manikandan Ariyan4Karuppusamy Rajan5Rangasamy Anandham6Department of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Tamil NaduDepartment of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Tamil NaduDepartment of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Tamil NaduDepartment of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Tamil NaduInstitute of Ecology and Earth Science, University of TartuDivision of Soil Science, ICAR-Indian Institute of Soil and Water Conservation Research CentreDepartment of Agricultural Microbiology, Tamil Nadu Agricultural UniversityAbstract Magnesium (Mg) an essential plant nutrient is widespread deficient in the acidic soils of Nilgiris of Tamil nadu, India. The vegetable yield and quality is especially affected due to deficiency of nutrients like Mg. This study investigates soil characteristics and bacterial diversity in the Nilgiris district of Tamil Nadu, India, with respect to Mg deficiency. The soil samples were collected from different vegetable growing regions of the Nilgiris to assess soil physiocochemical parameters, soil enzymes and soil Mg status. 16S rRNA gene-based metagenomic analysis used to investigate the functional potential and structural diversity of the bacterial communities in high Mg and low Mg deficiency soil. Results indicated mildly acidic soils with a sandy loam texture and high organic carbon content. While nitrogen (N), phosphorus (P), and potassium (K) levels were adequate, Mg deficiency was consistent. Soil enzymes such as dehydrogenase, acid phosphatase, urease and aryl sulfatase, varied across the soil samples. Additionally, 16S rRNA gene-based metagenomics analysis revealed the bacterial diversity and functional pathways in soils with high and low Mg deficiency. Low Mg levels were associated with increased bacterial richness, dominated by Proteobacteria, Gemmatimonadetes, Actinobacteria, Bacteroidetes, and Acidobacteria. Functional pathways related to carbon metabolism, amino acid biosynthesis, and various metabolic processes were more abundant in low Mg deficient soils. This research highlights the significant influence of Mg levels on bacterial diversity and functional potentials in acidic soils, providing insights into soil management strategies in Mg-deficient regions.https://doi.org/10.1038/s41598-025-87943-3Mg fertilizationSoil Enzyme activityBacterial communities16S rRNA sequencing |
| spellingShingle | Munmun Dash Subramaniam Thiyageshwari Duraisamy Selvi Haina K. V. Johnson Manikandan Ariyan Karuppusamy Rajan Rangasamy Anandham Unveiling microbial diversity in slightly and moderately magnesium deficient acidic soils Scientific Reports Mg fertilization Soil Enzyme activity Bacterial communities 16S rRNA sequencing |
| title | Unveiling microbial diversity in slightly and moderately magnesium deficient acidic soils |
| title_full | Unveiling microbial diversity in slightly and moderately magnesium deficient acidic soils |
| title_fullStr | Unveiling microbial diversity in slightly and moderately magnesium deficient acidic soils |
| title_full_unstemmed | Unveiling microbial diversity in slightly and moderately magnesium deficient acidic soils |
| title_short | Unveiling microbial diversity in slightly and moderately magnesium deficient acidic soils |
| title_sort | unveiling microbial diversity in slightly and moderately magnesium deficient acidic soils |
| topic | Mg fertilization Soil Enzyme activity Bacterial communities 16S rRNA sequencing |
| url | https://doi.org/10.1038/s41598-025-87943-3 |
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