Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis
An increase in the duration of marine heat waves (MHWs) has a serious negative impact on the survival of marine organisms. However, the impact of MHWs on Cyclina sinensis remains largely unknown. Here, this study assessed the effects of acute (25°C or 35°C for 12 h) and prolonged heatwave stress (no...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006471 |
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| author | Meimei Liu Zhichao Wang Hongwei Ni Wenqing Zhuo Gaoyuan Yuan Zhiguo Dong |
| author_facet | Meimei Liu Zhichao Wang Hongwei Ni Wenqing Zhuo Gaoyuan Yuan Zhiguo Dong |
| author_sort | Meimei Liu |
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| description | An increase in the duration of marine heat waves (MHWs) has a serious negative impact on the survival of marine organisms. However, the impact of MHWs on Cyclina sinensis remains largely unknown. Here, this study assessed the effects of acute (25°C or 35°C for 12 h) and prolonged heatwave stress (no heatwave (25°C for 4 weeks), short heatwave (25°C for 3 weeks then 1 week at 35°C), and long heatwave (35°C for 4 weeks)) on the physiology and metabolism of C. sinensis. Acute heat stress alters the expression of immune-related genes in the hepatopancreas, including LSZ1, DPP1, SRB1, Casp3, BIRC2, Arf, CLEC4M, CLEC10A and Gadd45β. The transcript levels of chaperones genes and antioxidant enzymes were up-regulated after acute heat stress treatment (35℃). Under prolonged heatwave stress challenge, HSP27, HSP70B2 and HSP90β mRNA in hepatopancreas and mantle were up-regulated in short heatwave group. The mRNA levels or activities of SOD in the hemolymph, hepatopancreas, and gills, as well as CAT in the hemolymph, hepatopancreas, and gills, were higher in clams from the short heatwave group than those from the no heatwave group. Surprisingly, the mRNA levels and activities of SOD or GSTP1 in the gills of clams in the short and long heatwave groups were significantly lower than those in the control group. The levels of GSH in the hepatopancreas and gills of clams in the short and long heatwave groups were lower than those in the no heatwave group, but the level of MDA was higher than that in the no heatwave group. Moreover, prolonged exposure to heatwaves resulted in the breakage and shortening of gill filaments and thinning of the hepatopancreas wall in clams. These findings suggest that heatwave cause oxidative stress and induce tissue damage in mollusks. |
| format | Article |
| id | doaj-art-78e406db0bbe411e9337bf442e33f45d |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-78e406db0bbe411e9337bf442e33f45d2025-02-06T05:12:08ZengElsevierAquaculture Reports2352-51342025-03-0140102559Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensisMeimei Liu0Zhichao Wang1Hongwei Ni2Wenqing Zhuo3Gaoyuan Yuan4Zhiguo Dong5Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, China; Jiangsu Key Laboratory of Marine Genetic Resources and Breeding, Jiangsu Ocean University, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Institute of Marine Resources Development, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, China; Jiangsu Key Laboratory of Marine Genetic Resources and Breeding, Jiangsu Ocean University, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Institute of Marine Resources Development, Lianyungang 222005, China; Corresponding author at: Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, China.An increase in the duration of marine heat waves (MHWs) has a serious negative impact on the survival of marine organisms. However, the impact of MHWs on Cyclina sinensis remains largely unknown. Here, this study assessed the effects of acute (25°C or 35°C for 12 h) and prolonged heatwave stress (no heatwave (25°C for 4 weeks), short heatwave (25°C for 3 weeks then 1 week at 35°C), and long heatwave (35°C for 4 weeks)) on the physiology and metabolism of C. sinensis. Acute heat stress alters the expression of immune-related genes in the hepatopancreas, including LSZ1, DPP1, SRB1, Casp3, BIRC2, Arf, CLEC4M, CLEC10A and Gadd45β. The transcript levels of chaperones genes and antioxidant enzymes were up-regulated after acute heat stress treatment (35℃). Under prolonged heatwave stress challenge, HSP27, HSP70B2 and HSP90β mRNA in hepatopancreas and mantle were up-regulated in short heatwave group. The mRNA levels or activities of SOD in the hemolymph, hepatopancreas, and gills, as well as CAT in the hemolymph, hepatopancreas, and gills, were higher in clams from the short heatwave group than those from the no heatwave group. Surprisingly, the mRNA levels and activities of SOD or GSTP1 in the gills of clams in the short and long heatwave groups were significantly lower than those in the control group. The levels of GSH in the hepatopancreas and gills of clams in the short and long heatwave groups were lower than those in the no heatwave group, but the level of MDA was higher than that in the no heatwave group. Moreover, prolonged exposure to heatwaves resulted in the breakage and shortening of gill filaments and thinning of the hepatopancreas wall in clams. These findings suggest that heatwave cause oxidative stress and induce tissue damage in mollusks.http://www.sciencedirect.com/science/article/pii/S2352513424006471Cyclina sinensisHeatwavesAntioxidant capacityHistology |
| spellingShingle | Meimei Liu Zhichao Wang Hongwei Ni Wenqing Zhuo Gaoyuan Yuan Zhiguo Dong Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis Aquaculture Reports Cyclina sinensis Heatwaves Antioxidant capacity Histology |
| title | Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis |
| title_full | Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis |
| title_fullStr | Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis |
| title_full_unstemmed | Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis |
| title_short | Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis |
| title_sort | adaptability of clams to prolonged heatwaves influences on gene expression antioxidant capacity and histology of chinese cyclina cyclina sinensis |
| topic | Cyclina sinensis Heatwaves Antioxidant capacity Histology |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006471 |
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