Isolation and molecular diagnosis of Staphylococcus aureus from eye infections in domestic cats in Mosul city.
Bacterial eye infections caused by Staphylococcus aureus are considered one of the most important infections that affect cats and cause serious health problems. Accordingly, this research was designed to diagnose S. aureus from eye infections in cats by bacteria isolation and molecular methods in Mo...
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| Main Authors: | , |
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| Format: | Article |
| Language: | Arabic |
| Published: |
University of Mosul, College of Veterinary Medicine
2025-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_186405_3684279dc90432456cb1f8915fd4c943.pdf |
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| Summary: | Bacterial eye infections caused by Staphylococcus aureus are considered one of the most important infections that affect cats and cause serious health problems. Accordingly, this research was designed to diagnose S. aureus from eye infections in cats by bacteria isolation and molecular methods in Mosul City, Iraq. One hundred eye swabs were collected from cat species that attended the pet clinics and were subjected to standard bacterial isolation and identification. Also, a disc diffusion method for antibiotic susceptibility was performed to determine antibiotic resistance. Further confirmation was done using polymerase chain reaction (PCR) targeting the nuc gene of S. aureus as molecular confirmation. This study showed that S. aureus was successfully isolated from 41/100 (41%) of the infected cats. In addition, the male cats represented 23/41 (56.1%), while the females represented 18/41 (43.9%). Furthermore, the cats aged ≥ 6 months were more susceptible to eye infections and recorded 29/41 (70.7%) than cats aged ≤ 6 months and recorded 12/41 (29.3%). The AST results showed significant antibiotic resistance, mainly against ampicillin and cefotaxime. Contrarily, all the isolates were completely susceptible to ciprofloxacin followed by levofloxacin. Also, PCR confirms S. aureus in all isolates with 279 bp for the nuc gene. In conclusion, S. aureus can cause eye infections in pet cats with significant importance, and the development of antibiotic resistance might have a human health impact, especially when these cats have close contact with humans. |
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| ISSN: | 1607-3894 2071-1255 |