Curdlan sulphate modulates protein synthesis and enhances NF-κB and C/EBP binding activity in HepG2 cells
In human hepatoma HepG2 cell line curdlan sulphate enhances basal and interleukin-6-stimulated fibrinogen and antichymotrypsin (ACT) synthesis, slightly increases basal ceruloplasmin production and exerts only minor effects on alpha-1-proteinase inhibitor and transferrin. Curdlan sulphate may, at le...
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| Format: | Article |
| Language: | English |
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Wiley
1997-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629359791947 |
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| _version_ | 1832549310255857664 |
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| author | A. Guzdek H. Rokita |
| author_facet | A. Guzdek H. Rokita |
| author_sort | A. Guzdek |
| collection | DOAJ |
| description | In human hepatoma HepG2 cell line curdlan sulphate enhances basal and interleukin-6-stimulated fibrinogen and antichymotrypsin (ACT) synthesis, slightly increases basal ceruloplasmin production and exerts only minor effects on alpha-1-proteinase inhibitor and transferrin. Curdlan sulphate may, at least in part, affect protein synthesis at a pretranslational level, as the expression of ACT mRNA was found to be increased, whereas intracellular enzyme, manganese superoxide dismutase mRNA level was decreased in the cell culture treated with curdlan sulphate. Gel mobility shift analysis revealed that curdlan sulphate increases the DNA binding activity of NF-κB and C/EBP, suggesting that these transcription factors may participate in the regulatory effects of curdlan sulphate in HepG2 cells. |
| format | Article |
| id | doaj-art-78ccb6cebba44f1891b7808ef9a96471 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-78ccb6cebba44f1891b7808ef9a964712025-02-03T06:11:37ZengWileyMediators of Inflammation0962-93511466-18611997-01-0161586310.1080/09629359791947Curdlan sulphate modulates protein synthesis and enhances NF-κB and C/EBP binding activity in HepG2 cellsA. Guzdek0H. Rokita1Institute of Molecular Biology, Jagiellonian University, Al. Mickiewicza 3, Krakow 31-120, PolandInstitute of Molecular Biology, Jagiellonian University, Al. Mickiewicza 3, Krakow 31-120, PolandIn human hepatoma HepG2 cell line curdlan sulphate enhances basal and interleukin-6-stimulated fibrinogen and antichymotrypsin (ACT) synthesis, slightly increases basal ceruloplasmin production and exerts only minor effects on alpha-1-proteinase inhibitor and transferrin. Curdlan sulphate may, at least in part, affect protein synthesis at a pretranslational level, as the expression of ACT mRNA was found to be increased, whereas intracellular enzyme, manganese superoxide dismutase mRNA level was decreased in the cell culture treated with curdlan sulphate. Gel mobility shift analysis revealed that curdlan sulphate increases the DNA binding activity of NF-κB and C/EBP, suggesting that these transcription factors may participate in the regulatory effects of curdlan sulphate in HepG2 cells.http://dx.doi.org/10.1080/09629359791947 |
| spellingShingle | A. Guzdek H. Rokita Curdlan sulphate modulates protein synthesis and enhances NF-κB and C/EBP binding activity in HepG2 cells Mediators of Inflammation |
| title | Curdlan sulphate modulates protein synthesis and enhances NF-κB and C/EBP binding activity in HepG2 cells |
| title_full | Curdlan sulphate modulates protein synthesis and enhances NF-κB and C/EBP binding activity in HepG2 cells |
| title_fullStr | Curdlan sulphate modulates protein synthesis and enhances NF-κB and C/EBP binding activity in HepG2 cells |
| title_full_unstemmed | Curdlan sulphate modulates protein synthesis and enhances NF-κB and C/EBP binding activity in HepG2 cells |
| title_short | Curdlan sulphate modulates protein synthesis and enhances NF-κB and C/EBP binding activity in HepG2 cells |
| title_sort | curdlan sulphate modulates protein synthesis and enhances nf κb and c ebp binding activity in hepg2 cells |
| url | http://dx.doi.org/10.1080/09629359791947 |
| work_keys_str_mv | AT aguzdek curdlansulphatemodulatesproteinsynthesisandenhancesnfkbandcebpbindingactivityinhepg2cells AT hrokita curdlansulphatemodulatesproteinsynthesisandenhancesnfkbandcebpbindingactivityinhepg2cells |