Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation
Thin endometrium (< 7 mm) could cause low clinical pregnancy, reduced live birth, increased spontaneous abortion, and decreased birth weight. However, the treatments for thin endometrium have not been well developed. In this study, we aim to determine the role of Pluronic F-127 (PF-127) encapsula...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/7819234 |
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| author | Shuling Zhou Yu Lei Ping Wang Jianying Chen Liting Zeng Ting Qu Martin Maldonado Jihua Huang Tingting Han Zina Wen Erpo Tian Xiangqian Meng Ying Zhong Jiang Gu |
| author_facet | Shuling Zhou Yu Lei Ping Wang Jianying Chen Liting Zeng Ting Qu Martin Maldonado Jihua Huang Tingting Han Zina Wen Erpo Tian Xiangqian Meng Ying Zhong Jiang Gu |
| author_sort | Shuling Zhou |
| collection | DOAJ |
| description | Thin endometrium (< 7 mm) could cause low clinical pregnancy, reduced live birth, increased spontaneous abortion, and decreased birth weight. However, the treatments for thin endometrium have not been well developed. In this study, we aim to determine the role of Pluronic F-127 (PF-127) encapsulation of human umbilical cord mesenchymal stem cells (hUC-MSCs) in the regeneration of thin endometrium and its underlying mechanism. Thin endometrium rat model was created by infusion of 95% ethanol. Thin endometrium modeled rat uterus were treated with saline, hUC-MSCs, PF-127, or hUC-MSCs plus PF-127 separately. Regenerated rat uterus was measured for gene expression levels of angiogenesis factors and histological morphology. Angiogenesis capacity of interleukin-1 beta (IL-1β)-primed hUC-MSCs was monitored via quantitative polymerase chain reaction (q-PCR), Luminex assay, and tube formation assay. Decreased endometrium thickness and gland number and increased inflammatory factor IL-1β were achieved in the thin endometrium rat model. Embedding of hUC-MSCs with PF-127 could prolong the hUC-MSCs retaining, which could further enhance endometrium thickness and gland number in the thin endometrium rat model via increasing angiogenesis capacity. Conditional medium derived from IL-1β-primed hUC-MSCs increased the concentration of angiogenesis factors (basic fibroblast growth factor (bFGF), vascular endothelial growth factors (VEGF), and hepatocyte growth factor (HGF)). Improvement in the thickness, number of glands, and newly generated blood vessels could be achieved by uterus endometrium treatment with PF-127 and hUC-MSCs transplantation. Local IL-1β stimulation-primed hUC-MSCs promoted the release of angiogenesis factors and may play a vital role on thin endometrium regeneration. |
| format | Article |
| id | doaj-art-78c9422b679f4253a25ccbd6fbf14a28 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-78c9422b679f4253a25ccbd6fbf14a282025-02-03T01:23:10ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/7819234Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β StimulationShuling Zhou0Yu Lei1Ping Wang2Jianying Chen3Liting Zeng4Ting Qu5Martin Maldonado6Jihua Huang7Tingting Han8Zina Wen9Erpo Tian10Xiangqian Meng11Ying Zhong12Jiang Gu13Jinxin Research Institute for Reproductive Medicine and GeneticsJinxin Research Institute for Reproductive Medicine and GeneticsJinxin Research Institute for Reproductive Medicine and GeneticsJinxin Research Institute for Reproductive Medicine and GeneticsDepartment of Pathology and Provincial Key Laboratory of Infectious Diseases and ImmunopathologyJinxin Research Institute for Reproductive Medicine and GeneticsJinxin Research Institute for Reproductive Medicine and GeneticsJinxin Research Institute for Reproductive Medicine and GeneticsJinxin Research Institute for Reproductive Medicine and GeneticsDepartment of AndrologyDepartment of AndrologyDepartment of EmbryologyDepartment of EmbryologyDepartment of Pathology and Provincial Key Laboratory of Infectious Diseases and ImmunopathologyThin endometrium (< 7 mm) could cause low clinical pregnancy, reduced live birth, increased spontaneous abortion, and decreased birth weight. However, the treatments for thin endometrium have not been well developed. In this study, we aim to determine the role of Pluronic F-127 (PF-127) encapsulation of human umbilical cord mesenchymal stem cells (hUC-MSCs) in the regeneration of thin endometrium and its underlying mechanism. Thin endometrium rat model was created by infusion of 95% ethanol. Thin endometrium modeled rat uterus were treated with saline, hUC-MSCs, PF-127, or hUC-MSCs plus PF-127 separately. Regenerated rat uterus was measured for gene expression levels of angiogenesis factors and histological morphology. Angiogenesis capacity of interleukin-1 beta (IL-1β)-primed hUC-MSCs was monitored via quantitative polymerase chain reaction (q-PCR), Luminex assay, and tube formation assay. Decreased endometrium thickness and gland number and increased inflammatory factor IL-1β were achieved in the thin endometrium rat model. Embedding of hUC-MSCs with PF-127 could prolong the hUC-MSCs retaining, which could further enhance endometrium thickness and gland number in the thin endometrium rat model via increasing angiogenesis capacity. Conditional medium derived from IL-1β-primed hUC-MSCs increased the concentration of angiogenesis factors (basic fibroblast growth factor (bFGF), vascular endothelial growth factors (VEGF), and hepatocyte growth factor (HGF)). Improvement in the thickness, number of glands, and newly generated blood vessels could be achieved by uterus endometrium treatment with PF-127 and hUC-MSCs transplantation. Local IL-1β stimulation-primed hUC-MSCs promoted the release of angiogenesis factors and may play a vital role on thin endometrium regeneration.http://dx.doi.org/10.1155/2022/7819234 |
| spellingShingle | Shuling Zhou Yu Lei Ping Wang Jianying Chen Liting Zeng Ting Qu Martin Maldonado Jihua Huang Tingting Han Zina Wen Erpo Tian Xiangqian Meng Ying Zhong Jiang Gu Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation Stem Cells International |
| title | Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation |
| title_full | Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation |
| title_fullStr | Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation |
| title_full_unstemmed | Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation |
| title_short | Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation |
| title_sort | human umbilical cord mesenchymal stem cells encapsulated with pluronic f 127 enhance the regeneration and angiogenesis of thin endometrium in rat via local il 1β stimulation |
| url | http://dx.doi.org/10.1155/2022/7819234 |
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