Inter-Laboratory Validation of Method to Determine Residual Enrofloxacin in Chicken Meat
An inter-laboratory study was performed to evaluate the performance of a method developed for the quantification of enrofloxacin in chicken meat. Liquid-liquid extraction combined with a clean-up procedure based on solid-phase extraction followed by a liquid chromatography-tandem mass spectrometric...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/6019549 |
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| author | Joo Hee Chung Kun Cho Seongnyeon Kim So Hyeon Jeon Jeoung Hwa Shin Jueun Lee Yun Gyong Ahn |
| author_facet | Joo Hee Chung Kun Cho Seongnyeon Kim So Hyeon Jeon Jeoung Hwa Shin Jueun Lee Yun Gyong Ahn |
| author_sort | Joo Hee Chung |
| collection | DOAJ |
| description | An inter-laboratory study was performed to evaluate the performance of a method developed for the quantification of enrofloxacin in chicken meat. Liquid-liquid extraction combined with a clean-up procedure based on solid-phase extraction followed by a liquid chromatography-tandem mass spectrometric method was used by three individual laboratories. All the investigated results of calibration curves and limits of quantification were within the acceptable range for regulatory testing of enrofloxacin. The three laboratories received blind a certified reference material to analyze in triplicate and assess using statistical analysis. From the results, no statistical differences were found between the laboratories in the precision of the method. Additionally, all the results of the z-score, which is an indication of fixed interval bias criteria for accuracy from the laboratories, fell within the allowable limits (±2σ). Based on this proficiency testing by inter-laboratory comparisons, the analytical method including the sample preparation step was proven to be applicable. |
| format | Article |
| id | doaj-art-787b816708aa43108a20cdddce3321a9 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-787b816708aa43108a20cdddce3321a92025-02-03T06:44:34ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792018-01-01201810.1155/2018/60195496019549Inter-Laboratory Validation of Method to Determine Residual Enrofloxacin in Chicken MeatJoo Hee Chung0Kun Cho1Seongnyeon Kim2So Hyeon Jeon3Jeoung Hwa Shin4Jueun Lee5Yun Gyong Ahn6Seoul Center, Korea Basic Science Institute, Seoul 02841, Republic of KoreaBiomedical Omics Group, Korea Basic Science Institute, Ochang 28119, Republic of KoreaBiomedical Omics Group, Korea Basic Science Institute, Ochang 28119, Republic of KoreaWestern Seoul Center, Korea Basic Science Institute, Seoul 03759, Republic of KoreaSeoul Center, Korea Basic Science Institute, Seoul 02841, Republic of KoreaWestern Seoul Center, Korea Basic Science Institute, Seoul 03759, Republic of KoreaWestern Seoul Center, Korea Basic Science Institute, Seoul 03759, Republic of KoreaAn inter-laboratory study was performed to evaluate the performance of a method developed for the quantification of enrofloxacin in chicken meat. Liquid-liquid extraction combined with a clean-up procedure based on solid-phase extraction followed by a liquid chromatography-tandem mass spectrometric method was used by three individual laboratories. All the investigated results of calibration curves and limits of quantification were within the acceptable range for regulatory testing of enrofloxacin. The three laboratories received blind a certified reference material to analyze in triplicate and assess using statistical analysis. From the results, no statistical differences were found between the laboratories in the precision of the method. Additionally, all the results of the z-score, which is an indication of fixed interval bias criteria for accuracy from the laboratories, fell within the allowable limits (±2σ). Based on this proficiency testing by inter-laboratory comparisons, the analytical method including the sample preparation step was proven to be applicable.http://dx.doi.org/10.1155/2018/6019549 |
| spellingShingle | Joo Hee Chung Kun Cho Seongnyeon Kim So Hyeon Jeon Jeoung Hwa Shin Jueun Lee Yun Gyong Ahn Inter-Laboratory Validation of Method to Determine Residual Enrofloxacin in Chicken Meat International Journal of Analytical Chemistry |
| title | Inter-Laboratory Validation of Method to Determine Residual Enrofloxacin in Chicken Meat |
| title_full | Inter-Laboratory Validation of Method to Determine Residual Enrofloxacin in Chicken Meat |
| title_fullStr | Inter-Laboratory Validation of Method to Determine Residual Enrofloxacin in Chicken Meat |
| title_full_unstemmed | Inter-Laboratory Validation of Method to Determine Residual Enrofloxacin in Chicken Meat |
| title_short | Inter-Laboratory Validation of Method to Determine Residual Enrofloxacin in Chicken Meat |
| title_sort | inter laboratory validation of method to determine residual enrofloxacin in chicken meat |
| url | http://dx.doi.org/10.1155/2018/6019549 |
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