MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA
Background: Isolates of Klebsiella pneumoniae resistant to Carbapenem appears to be most commonly transmitted by hospitalized patients. This may cause infections which lead to mortality. Therefore, studies on molecular level will help to gain more information and be able to know their cross-transmis...
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| Format: | Article |
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Universitas Airlangga
2023-11-01
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| Series: | Journal of Vocational Health Studies |
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| Online Access: | https://e-journal.unair.ac.id/JVHS/article/view/38651 |
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| author | Swapna C. Senan Jithu Paul Jacob Ramani Bhai |
| author_facet | Swapna C. Senan Jithu Paul Jacob Ramani Bhai |
| author_sort | Swapna C. Senan |
| collection | DOAJ |
| description | Background: Isolates of Klebsiella pneumoniae resistant to Carbapenem appears to be most commonly transmitted by hospitalized patients. This may cause infections which lead to mortality. Therefore, studies on molecular level will help to gain more information and be able to know their cross-transmission and resistance mechanisms among these isolates showing resistance. Purpose: Investigate the molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from various clinical samples. Klebsiella pneumoniae are aerobic and Gram-negative bacilli found in the gastrointestinal tract of humans and other animals and also as saprophytes in soil. Method: In this study, 401 bacteria of Klebsiella isolates were taken from various clinical samples according to standard protocol. Carbapenem-resistant genes of Klebsiella pneumoniae (CRK) isolates were detected using multiplex polymerase chain reaction (PCR). Result: The twelve isolates resistant to carbapenem were confirmed phenotypically and were confirmed using a multiplex Polymerase Chain Reaction. Conclusion: The present study concluded that identification of Carbapenem-resistant genes of Klebsiella pneumoniae (KPC) could help find out the routes of dissemination of the species and may control its spread within the hospital premises. |
| format | Article |
| id | doaj-art-7841fdbf386b4a6d86f8eacd7393a9e1 |
| institution | Kabale University |
| issn | 2580-7161 2580-717X |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Vocational Health Studies |
| spelling | doaj-art-7841fdbf386b4a6d86f8eacd7393a9e12025-02-03T00:36:04ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2023-11-0172838910.20473/jvhs.V7.I2.2023.83-8936721MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIASwapna C. Senan0https://orcid.org/0000-0001-9949-9785Jithu Paul Jacob1Ramani Bhai2Department of Microbiology, Sree Gokulam Medical College, Trivandrum, KeralaResearch Department of Fisheries and Aquaculture, St. Albert’s College (Autonomous), Ernakulam, Kerala, IndiaDepartment of Microbiology, Sree Gokulam Medical College, Trivandrum, Kerala Background: Isolates of Klebsiella pneumoniae resistant to Carbapenem appears to be most commonly transmitted by hospitalized patients. This may cause infections which lead to mortality. Therefore, studies on molecular level will help to gain more information and be able to know their cross-transmission and resistance mechanisms among these isolates showing resistance. Purpose: Investigate the molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from various clinical samples. Klebsiella pneumoniae are aerobic and Gram-negative bacilli found in the gastrointestinal tract of humans and other animals and also as saprophytes in soil. Method: In this study, 401 bacteria of Klebsiella isolates were taken from various clinical samples according to standard protocol. Carbapenem-resistant genes of Klebsiella pneumoniae (CRK) isolates were detected using multiplex polymerase chain reaction (PCR). Result: The twelve isolates resistant to carbapenem were confirmed phenotypically and were confirmed using a multiplex Polymerase Chain Reaction. Conclusion: The present study concluded that identification of Carbapenem-resistant genes of Klebsiella pneumoniae (KPC) could help find out the routes of dissemination of the species and may control its spread within the hospital premises.https://e-journal.unair.ac.id/JVHS/article/view/38651carbapenem resistanceklebsiella pneumoniaepolymerase chain reactionmodified hodge testcarba np test |
| spellingShingle | Swapna C. Senan Jithu Paul Jacob Ramani Bhai MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA Journal of Vocational Health Studies carbapenem resistance klebsiella pneumoniae polymerase chain reaction modified hodge test carba np test |
| title | MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA |
| title_full | MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA |
| title_fullStr | MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA |
| title_full_unstemmed | MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA |
| title_short | MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA |
| title_sort | molecular characterization of carbapenem resistant klebsiella pneumoniae at a tertiary care laboratory in kerala india |
| topic | carbapenem resistance klebsiella pneumoniae polymerase chain reaction modified hodge test carba np test |
| url | https://e-journal.unair.ac.id/JVHS/article/view/38651 |
| work_keys_str_mv | AT swapnacsenan molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeatatertiarycarelaboratoryinkeralaindia AT jithupauljacob molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeatatertiarycarelaboratoryinkeralaindia AT ramanibhai molecularcharacterizationofcarbapenemresistantklebsiellapneumoniaeatatertiarycarelaboratoryinkeralaindia |