The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis
Helicobacter pylori (H. pylori) is the main pathogenic factor of gastric cancer, chronic gastritis, and other gastric diseases. It has been found that Callicarpa nudiflora (CN) as an air-dried leaf extract has a broad-spectrum antibacterial effect. This study aims to examine the effect of CN on H. p...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/5469236 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558392604884992 |
|---|---|
| author | Lili Li Bo Bao Xingxing Chai Xiaoyu Chen Xiaohua Su Shixiu Feng Xiaohui Zhu |
| author_facet | Lili Li Bo Bao Xingxing Chai Xiaoyu Chen Xiaohua Su Shixiu Feng Xiaohui Zhu |
| author_sort | Lili Li |
| collection | DOAJ |
| description | Helicobacter pylori (H. pylori) is the main pathogenic factor of gastric cancer, chronic gastritis, and other gastric diseases. It has been found that Callicarpa nudiflora (CN) as an air-dried leaf extract has a broad-spectrum antibacterial effect. This study aims to examine the effect of CN on H. pylori-infected GES-1 cells in vitro and elucidate its underlying mechanism by extracting active ingredients from air-dried leaves. GES-1 cells were cocultured with HPSS1 at MOI = 100 : 1 and treated with different concentrations of CN (100 and 200 μg/ml). Results showed that CN can significantly reduce cellular LDH leakage and attenuate H. pylori-induced cell apoptosis and ROS production in GSE-1 cells, so as to protect gastric epithelial cells from damage by H. pylori. CN can also inhibit the secretion of inflammatory factors, such as TNF-α, IL-1β, IL-6, and IL-8. After CN treatment, the expression levels of active caspase-1, PYCARD, and NLRP3 were remarkably decreased in the treatment groups compared with the model group. To sum up, CN is highly protective against H. pylori-induced cell damage and apoptosis; CN can depress NLRP3 inflammasome activation and ROS production via the ROS/NLRP3/caspase-1/IL-1β signaling axis to suppress H. pylori-triggered inflammatory response and pyroptosis. |
| format | Article |
| id | doaj-art-7827bd1dceff4788a0727c120c91106c |
| institution | Kabale University |
| issn | 1918-1493 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-7827bd1dceff4788a0727c120c91106c2025-02-03T01:32:26ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1918-14932022-01-01202210.1155/2022/5469236The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling AxisLili Li0Bo Bao1Xingxing Chai2Xiaoyu Chen3Xiaohua Su4Shixiu Feng5Xiaohui Zhu6Laboratory Animal CentreDepartment of PathophysiologyLaboratory Animal CentreDepartment of PathophysiologyLaboratory Animal CentreKey Laboratory of South Subtropical Plant DiversityCollege of PharmacyHelicobacter pylori (H. pylori) is the main pathogenic factor of gastric cancer, chronic gastritis, and other gastric diseases. It has been found that Callicarpa nudiflora (CN) as an air-dried leaf extract has a broad-spectrum antibacterial effect. This study aims to examine the effect of CN on H. pylori-infected GES-1 cells in vitro and elucidate its underlying mechanism by extracting active ingredients from air-dried leaves. GES-1 cells were cocultured with HPSS1 at MOI = 100 : 1 and treated with different concentrations of CN (100 and 200 μg/ml). Results showed that CN can significantly reduce cellular LDH leakage and attenuate H. pylori-induced cell apoptosis and ROS production in GSE-1 cells, so as to protect gastric epithelial cells from damage by H. pylori. CN can also inhibit the secretion of inflammatory factors, such as TNF-α, IL-1β, IL-6, and IL-8. After CN treatment, the expression levels of active caspase-1, PYCARD, and NLRP3 were remarkably decreased in the treatment groups compared with the model group. To sum up, CN is highly protective against H. pylori-induced cell damage and apoptosis; CN can depress NLRP3 inflammasome activation and ROS production via the ROS/NLRP3/caspase-1/IL-1β signaling axis to suppress H. pylori-triggered inflammatory response and pyroptosis.http://dx.doi.org/10.1155/2022/5469236 |
| spellingShingle | Lili Li Bo Bao Xingxing Chai Xiaoyu Chen Xiaohua Su Shixiu Feng Xiaohui Zhu The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis |
| title_full | The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis |
| title_fullStr | The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis |
| title_full_unstemmed | The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis |
| title_short | The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis |
| title_sort | anti inflammatory effect of callicarpa nudiflora extract on h pylori infected ges 1 cells through the inhibition of ros nlrp3 caspase 1 il 1β signaling axis |
| url | http://dx.doi.org/10.1155/2022/5469236 |
| work_keys_str_mv | AT lilili theantiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT bobao theantiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT xingxingchai theantiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT xiaoyuchen theantiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT xiaohuasu theantiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT shixiufeng theantiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT xiaohuizhu theantiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT lilili antiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT bobao antiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT xingxingchai antiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT xiaoyuchen antiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT xiaohuasu antiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT shixiufeng antiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis AT xiaohuizhu antiinflammatoryeffectofcallicarpanudifloraextractonhpyloriinfectedges1cellsthroughtheinhibitionofrosnlrp3caspase1il1bsignalingaxis |