Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells
Coffee is commonly consumed beverage in the world and it has been suggested to have beneficial effect. Chlorogenic acids (CGAs) are main ingredient of coffee beans which has been extensively used in nutraceuticals and medicine. Recently, various therapeutic effects of chlorogenic acids have been inv...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2017-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453017300356 |
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| author | K. Gouthamchandra H.V. Sudeep B.J. Venkatesh K. Shyam Prasad |
| author_facet | K. Gouthamchandra H.V. Sudeep B.J. Venkatesh K. Shyam Prasad |
| author_sort | K. Gouthamchandra |
| collection | DOAJ |
| description | Coffee is commonly consumed beverage in the world and it has been suggested to have beneficial effect. Chlorogenic acids (CGAs) are main ingredient of coffee beans which has been extensively used in nutraceuticals and medicine. Recently, various therapeutic effects of chlorogenic acids have been investigated. However, there are limited studies to investigate its anticancer properties. In the present study, we have used chlorogenic acid complex (CGA7) a decaffeinated water soluble green coffee bean extract to evaluate its cytotoxic effect on human and mouse cancer cell lines by using different approaches. From our results we found CGA7 treatment induces cell death in a dose and time dependent manner in different cancer cell lines. Further, CGA7 induced apoptosis was characterized by DNA fragmentation, PARP-1 cleavage, caspase-9 activation, and down regulation of Bcl-2, an anti-apoptotic protein and up regulation of pro-apoptotic protein BAX. Overall findings indicated that CGA7 complex a potent anticancer molecule found in green coffee beans could be a safe bioactive ingredient for prevention of cancer. |
| format | Article |
| id | doaj-art-77ea621b52ba465484a7b6f39a844226 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-77ea621b52ba465484a7b6f39a8442262025-02-03T04:07:02ZengTsinghua University PressFood Science and Human Wellness2213-45302017-09-016314715310.1016/j.fshw.2017.06.001Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cellsK. GouthamchandraH.V. SudeepB.J. VenkateshK. Shyam PrasadCoffee is commonly consumed beverage in the world and it has been suggested to have beneficial effect. Chlorogenic acids (CGAs) are main ingredient of coffee beans which has been extensively used in nutraceuticals and medicine. Recently, various therapeutic effects of chlorogenic acids have been investigated. However, there are limited studies to investigate its anticancer properties. In the present study, we have used chlorogenic acid complex (CGA7) a decaffeinated water soluble green coffee bean extract to evaluate its cytotoxic effect on human and mouse cancer cell lines by using different approaches. From our results we found CGA7 treatment induces cell death in a dose and time dependent manner in different cancer cell lines. Further, CGA7 induced apoptosis was characterized by DNA fragmentation, PARP-1 cleavage, caspase-9 activation, and down regulation of Bcl-2, an anti-apoptotic protein and up regulation of pro-apoptotic protein BAX. Overall findings indicated that CGA7 complex a potent anticancer molecule found in green coffee beans could be a safe bioactive ingredient for prevention of cancer.http://www.sciencedirect.com/science/article/pii/S2213453017300356ApoptosisChlorogenic acid complexPARP-1Green coffee beansDNA fragmentation |
| spellingShingle | K. Gouthamchandra H.V. Sudeep B.J. Venkatesh K. Shyam Prasad Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells Food Science and Human Wellness Apoptosis Chlorogenic acid complex PARP-1 Green coffee beans DNA fragmentation |
| title | Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells |
| title_full | Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells |
| title_fullStr | Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells |
| title_full_unstemmed | Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells |
| title_short | Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells |
| title_sort | chlorogenic acid complex cga7 standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer hct 116 cells |
| topic | Apoptosis Chlorogenic acid complex PARP-1 Green coffee beans DNA fragmentation |
| url | http://www.sciencedirect.com/science/article/pii/S2213453017300356 |
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