Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor α and β
Estradiol (E2) promotes metastatic propensity. However, the detailed mechanism remains largely unknown. E-cadherin, vimentin, and MMP-9 play a dominant role in the metastatic process. We aimed to investigate the effects of E2 on metastatic potential of PTC cell line BCPAP and on E-cadherin, vimentin...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2013/941568 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562412048351232 |
|---|---|
| author | Wenwu Dong Hao Zhang Jing Li Haixia Guan Liang He Zhihong Wang Zhongyan Shan Weiping Teng |
| author_facet | Wenwu Dong Hao Zhang Jing Li Haixia Guan Liang He Zhihong Wang Zhongyan Shan Weiping Teng |
| author_sort | Wenwu Dong |
| collection | DOAJ |
| description | Estradiol (E2) promotes metastatic propensity. However, the detailed mechanism remains largely unknown. E-cadherin, vimentin, and MMP-9 play a dominant role in the metastatic process. We aimed to investigate the effects of E2 on metastatic potential of PTC cell line BCPAP and on E-cadherin, vimentin, and MMP-9 protein expression. PTC cell line BCPAP was evaluated for the presence of estrogen receptor (ER) by western blot analysis. The effects of E2, PPT (a potent ERα-selective agonist), and DPN (a potent ERβ-selective agonist) on modulation of metastatic phenotype were determined by using in vitro scratch wound assay and invasion assay. In addition, the effects on E-cadherin, vimentin, and matrix metalloproteinase-9 (MMP-9) protein expression were evaluated by Western blot analysis. We found that BCPAP cells expressed ERα and ERβ. E2 and PPT enhanced, but DPN inhibited, the migration and invasion of BCPAP cells in an in vitro experimental model system that is modulated by E-cadherin, vimentin, and MMP-9. These findings indicate that E2 induces the metastatic potential of BCPAP cells through ERα and ERβ. The two ER subtypes play differential roles in modulation of BCPAP cell metastasis and the related molecule expressions including E-cadherin, vimentin, and MMP-9. |
| format | Article |
| id | doaj-art-77c6f6003df447a286a9e27f6111d58a |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-77c6f6003df447a286a9e27f6111d58a2025-02-03T01:22:39ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/941568941568Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor α and βWenwu Dong0Hao Zhang1Jing Li2Haixia Guan3Liang He4Zhihong Wang5Zhongyan Shan6Weiping Teng7Department of General Surgery, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, ChinaDepartment of General Surgery, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, ChinaDepartment of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, ChinaDepartment of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, ChinaDepartment of General Surgery, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, ChinaDepartment of General Surgery, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, ChinaDepartment of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, ChinaDepartment of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, ChinaEstradiol (E2) promotes metastatic propensity. However, the detailed mechanism remains largely unknown. E-cadherin, vimentin, and MMP-9 play a dominant role in the metastatic process. We aimed to investigate the effects of E2 on metastatic potential of PTC cell line BCPAP and on E-cadherin, vimentin, and MMP-9 protein expression. PTC cell line BCPAP was evaluated for the presence of estrogen receptor (ER) by western blot analysis. The effects of E2, PPT (a potent ERα-selective agonist), and DPN (a potent ERβ-selective agonist) on modulation of metastatic phenotype were determined by using in vitro scratch wound assay and invasion assay. In addition, the effects on E-cadherin, vimentin, and matrix metalloproteinase-9 (MMP-9) protein expression were evaluated by Western blot analysis. We found that BCPAP cells expressed ERα and ERβ. E2 and PPT enhanced, but DPN inhibited, the migration and invasion of BCPAP cells in an in vitro experimental model system that is modulated by E-cadherin, vimentin, and MMP-9. These findings indicate that E2 induces the metastatic potential of BCPAP cells through ERα and ERβ. The two ER subtypes play differential roles in modulation of BCPAP cell metastasis and the related molecule expressions including E-cadherin, vimentin, and MMP-9.http://dx.doi.org/10.1155/2013/941568 |
| spellingShingle | Wenwu Dong Hao Zhang Jing Li Haixia Guan Liang He Zhihong Wang Zhongyan Shan Weiping Teng Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor α and β International Journal of Endocrinology |
| title | Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor α and β |
| title_full | Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor α and β |
| title_fullStr | Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor α and β |
| title_full_unstemmed | Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor α and β |
| title_short | Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor α and β |
| title_sort | estrogen induces metastatic potential of papillary thyroid cancer cells through estrogen receptor α and β |
| url | http://dx.doi.org/10.1155/2013/941568 |
| work_keys_str_mv | AT wenwudong estrogeninducesmetastaticpotentialofpapillarythyroidcancercellsthroughestrogenreceptoraandb AT haozhang estrogeninducesmetastaticpotentialofpapillarythyroidcancercellsthroughestrogenreceptoraandb AT jingli estrogeninducesmetastaticpotentialofpapillarythyroidcancercellsthroughestrogenreceptoraandb AT haixiaguan estrogeninducesmetastaticpotentialofpapillarythyroidcancercellsthroughestrogenreceptoraandb AT lianghe estrogeninducesmetastaticpotentialofpapillarythyroidcancercellsthroughestrogenreceptoraandb AT zhihongwang estrogeninducesmetastaticpotentialofpapillarythyroidcancercellsthroughestrogenreceptoraandb AT zhongyanshan estrogeninducesmetastaticpotentialofpapillarythyroidcancercellsthroughestrogenreceptoraandb AT weipingteng estrogeninducesmetastaticpotentialofpapillarythyroidcancercellsthroughestrogenreceptoraandb |