Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphoma
Primary central nervous system diffused large B-cell lymphoma (PCNS-DLBCL) is a rare type of non-Hodgkin lymphoma restricted to the central nervous system (CNS). To explore its specific pathogenesis and therapeutic targets, we performed multi-omics sequencing on tumor samples from patients diagnosed...
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Elsevier
2025-02-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S147655862400160X |
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| author | Shu Wang Hong Chen Bo Dai Kang Zheng Jiajun Zheng Yuqi Zhu Yan Yuan Tianling Ding Qian Wang Liqian Xie Rui Feng Fengping Zhu Jianbin Xiang Weiqun Ding Hong Ding Yuan Li Xiaodong Gu Kunpeng Wu Yifan Yuan Jianping Song Dongxiao Zhuang Haoshu Zhong Hanfeng Wu Ying Mao Tong Chen |
| author_facet | Shu Wang Hong Chen Bo Dai Kang Zheng Jiajun Zheng Yuqi Zhu Yan Yuan Tianling Ding Qian Wang Liqian Xie Rui Feng Fengping Zhu Jianbin Xiang Weiqun Ding Hong Ding Yuan Li Xiaodong Gu Kunpeng Wu Yifan Yuan Jianping Song Dongxiao Zhuang Haoshu Zhong Hanfeng Wu Ying Mao Tong Chen |
| author_sort | Shu Wang |
| collection | DOAJ |
| description | Primary central nervous system diffused large B-cell lymphoma (PCNS-DLBCL) is a rare type of non-Hodgkin lymphoma restricted to the central nervous system (CNS). To explore its specific pathogenesis and therapeutic targets, we performed multi-omics sequencing on tumor samples from patients diagnosed with PCNS-DLBCL, secondary CNS-DLBCL or extracranial (ec) DLBCL.By single-cell RNA sequencing, highly proliferated and dark zone (DZ)-related B cell subclusters, MKI67_B1, PTTG1_B2 and BTG1_B3, were predominant significantly in PCNS-DLBCL. Compared to SCNS-DLBCL and ecDLBCL, an immune-suppressive tumor microenvironment was observed in PCNS-DLBCL by analysis of immune-stimulating/inhibitory ligand‒receptor (L-R) pairs. By performing whole-exome sequencing in 93 patients, mutations enriched in BCR-NFkB and TLR pathways and the cooperation of these two pathways were found to be predominant in PCNS-DLBCL comparing to nonGCB-ecDLBCL. In summary, our study provides comprehensive insights into the transcriptomic and genetic characteristics of PCNS-DLBCL in contrast to ecDLBCL and will help dissect the oncogenic mechanism of this disease. |
| format | Article |
| id | doaj-art-77b6d8d53b84434f8bb6e366c8d11bee |
| institution | Kabale University |
| issn | 1476-5586 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj-art-77b6d8d53b84434f8bb6e366c8d11bee2025-02-03T04:16:36ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55862025-02-0160101119Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphomaShu Wang0Hong Chen1Bo Dai2Kang Zheng3Jiajun Zheng4Yuqi Zhu5Yan Yuan6Tianling Ding7Qian Wang8Liqian Xie9Rui Feng10Fengping Zhu11Jianbin Xiang12Weiqun Ding13Hong Ding14Yuan Li15Xiaodong Gu16Kunpeng Wu17Yifan Yuan18Jianping Song19Dongxiao Zhuang20Haoshu Zhong21Hanfeng Wu22Ying Mao23Tong Chen24Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Pathology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Radiology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China; National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Neurosurgical Institute of Fudan University, Huashan Hospital, Shanghai 200040, PR ChinaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Ultrasound, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Radiology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Gastrointestinal Surgery, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China; National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China; National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Neurosurgical Institute of Fudan University, Huashan Hospital, Shanghai 200040, PR China; Shanghai Clinical Medical Center of Neurosurgery, Shanghai 200040, PR China; Shanghai Key Laboratory of Brain Function Restoration and Neural Regeneration, Shanghai 200040, PR ChinaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR ChinaDepartment of Neurosurgery, Shanghai Gamma Hospital, Shanghai 200235, PR China; Corresponding author: Department of Neurosurgery, Shanghai Gamma Hospital, 518 Wuzhong East Road, Shanghai 200235, PR China.Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Corresponding author: Department of Neurosurgery, Huashan Hospital, Fudan University. 12 Wulumuqi Middle Road, Shanghai 200040, PR China.Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Corresponding author: Department of Hematology, Huashan Hospital, Fudan University. 12 Wulumuqi Middle Road, Shanghai 200040, PR China.Primary central nervous system diffused large B-cell lymphoma (PCNS-DLBCL) is a rare type of non-Hodgkin lymphoma restricted to the central nervous system (CNS). To explore its specific pathogenesis and therapeutic targets, we performed multi-omics sequencing on tumor samples from patients diagnosed with PCNS-DLBCL, secondary CNS-DLBCL or extracranial (ec) DLBCL.By single-cell RNA sequencing, highly proliferated and dark zone (DZ)-related B cell subclusters, MKI67_B1, PTTG1_B2 and BTG1_B3, were predominant significantly in PCNS-DLBCL. Compared to SCNS-DLBCL and ecDLBCL, an immune-suppressive tumor microenvironment was observed in PCNS-DLBCL by analysis of immune-stimulating/inhibitory ligand‒receptor (L-R) pairs. By performing whole-exome sequencing in 93 patients, mutations enriched in BCR-NFkB and TLR pathways and the cooperation of these two pathways were found to be predominant in PCNS-DLBCL comparing to nonGCB-ecDLBCL. In summary, our study provides comprehensive insights into the transcriptomic and genetic characteristics of PCNS-DLBCL in contrast to ecDLBCL and will help dissect the oncogenic mechanism of this disease.http://www.sciencedirect.com/science/article/pii/S147655862400160XDiffuse large B cell lymphomaCentral nervous systemSingle-cell RNA sequencingGenomicsTumor Microenvironment |
| spellingShingle | Shu Wang Hong Chen Bo Dai Kang Zheng Jiajun Zheng Yuqi Zhu Yan Yuan Tianling Ding Qian Wang Liqian Xie Rui Feng Fengping Zhu Jianbin Xiang Weiqun Ding Hong Ding Yuan Li Xiaodong Gu Kunpeng Wu Yifan Yuan Jianping Song Dongxiao Zhuang Haoshu Zhong Hanfeng Wu Ying Mao Tong Chen Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphoma Neoplasia: An International Journal for Oncology Research Diffuse large B cell lymphoma Central nervous system Single-cell RNA sequencing Genomics Tumor Microenvironment |
| title | Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphoma |
| title_full | Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphoma |
| title_fullStr | Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphoma |
| title_full_unstemmed | Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphoma |
| title_short | Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphoma |
| title_sort | comparison of differences in transcriptional and genetic profiles between intra central nervous system and extra central nervous system large b cell lymphoma |
| topic | Diffuse large B cell lymphoma Central nervous system Single-cell RNA sequencing Genomics Tumor Microenvironment |
| url | http://www.sciencedirect.com/science/article/pii/S147655862400160X |
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