Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathway
Weaning stress causes substantial economic loss in the swine industry. Moreover, weaning-induced intestinal barrier damage and dysfunction of the gut-liver axis are associated with reduced growth performance in piglets. Metasilicate-based alkaline mineral water (AMW) has shown potential therapeutic...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Animal Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654524001446 |
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| author | Jian Chen Kanwar K. Malhi Xiaowei Li Xiangwen Xu Jianxun Kang Bichen Zhao Yaru Xu Xuenan Li Jinlong Li |
| author_facet | Jian Chen Kanwar K. Malhi Xiaowei Li Xiangwen Xu Jianxun Kang Bichen Zhao Yaru Xu Xuenan Li Jinlong Li |
| author_sort | Jian Chen |
| collection | DOAJ |
| description | Weaning stress causes substantial economic loss in the swine industry. Moreover, weaning-induced intestinal barrier damage and dysfunction of the gut-liver axis are associated with reduced growth performance in piglets. Metasilicate-based alkaline mineral water (AMW) has shown potential therapeutic effects on gastrointestinal disorders; however, the mechanisms involved and their overall effects on the gut-liver axis have not been explored. Here, sodium metasilicate (SMS) was used to prepare metasilicate-based AMW (basal water + 500 mg/L SMS). A total of 240 newly weaned piglets were allocated to the Control and SMS groups (6 replicate pens per group and 20 piglets per pen) for a 15-day trial period. Histopathological evaluations were conducted using hematoxylin and eosin staining. To analyze the composition of the gut microbiota, 16S rRNA PacBio SMRT Gene Full-Length Sequencing was performed. Western blotting and immunofluorescence were employed to assess protein expression levels. Our results indicated that metasilicate-based AMW effectively alleviated weaning-induced colonic or liver morphological injury and inflammatory response, as well as liver cholesterol metabolism disorders. Further analysis showed that metasilicate-based AMW promoted deoxycholic acid (DCA) biosynthesis by increasing the abundance of Lactobacillus_delbrueckii in the colon (P < 0.001). This consequently improved weaning-induced colon and liver injury and dysfunction through the DCA-secondary bile acid (SBA) receptors (SBAR)-nuclear factor-kappaB (NF-κB)/NOD-like receptor family pyrin domain-containing 3 (NLRP3) pathways. Growth performance parameters, including final body weight (P = 0.034) and average daily gain (P < 0.001), in the SMS group were significantly higher than those in the Control group. Therefore, metasilicate-based AMW maintains gut-liver axis homeostasis by regulating the microbiota-mediated SBA-SBAR pathway in piglets under weaning stress. Our research provides a new strategy for mitigating stress-induced gut-liver axis dysfunction in weaned piglets. |
| format | Article |
| id | doaj-art-77aac5c1075a4a4daf9dc8745a811ff5 |
| institution | Kabale University |
| issn | 2405-6545 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj-art-77aac5c1075a4a4daf9dc8745a811ff52025-01-31T05:11:56ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452025-03-012095109Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathwayJian Chen0Kanwar K. Malhi1Xiaowei Li2Xiangwen Xu3Jianxun Kang4Bichen Zhao5Yaru Xu6Xuenan Li7Jinlong Li8College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin 150030, China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin 150030, China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.Weaning stress causes substantial economic loss in the swine industry. Moreover, weaning-induced intestinal barrier damage and dysfunction of the gut-liver axis are associated with reduced growth performance in piglets. Metasilicate-based alkaline mineral water (AMW) has shown potential therapeutic effects on gastrointestinal disorders; however, the mechanisms involved and their overall effects on the gut-liver axis have not been explored. Here, sodium metasilicate (SMS) was used to prepare metasilicate-based AMW (basal water + 500 mg/L SMS). A total of 240 newly weaned piglets were allocated to the Control and SMS groups (6 replicate pens per group and 20 piglets per pen) for a 15-day trial period. Histopathological evaluations were conducted using hematoxylin and eosin staining. To analyze the composition of the gut microbiota, 16S rRNA PacBio SMRT Gene Full-Length Sequencing was performed. Western blotting and immunofluorescence were employed to assess protein expression levels. Our results indicated that metasilicate-based AMW effectively alleviated weaning-induced colonic or liver morphological injury and inflammatory response, as well as liver cholesterol metabolism disorders. Further analysis showed that metasilicate-based AMW promoted deoxycholic acid (DCA) biosynthesis by increasing the abundance of Lactobacillus_delbrueckii in the colon (P < 0.001). This consequently improved weaning-induced colon and liver injury and dysfunction through the DCA-secondary bile acid (SBA) receptors (SBAR)-nuclear factor-kappaB (NF-κB)/NOD-like receptor family pyrin domain-containing 3 (NLRP3) pathways. Growth performance parameters, including final body weight (P = 0.034) and average daily gain (P < 0.001), in the SMS group were significantly higher than those in the Control group. Therefore, metasilicate-based AMW maintains gut-liver axis homeostasis by regulating the microbiota-mediated SBA-SBAR pathway in piglets under weaning stress. Our research provides a new strategy for mitigating stress-induced gut-liver axis dysfunction in weaned piglets.http://www.sciencedirect.com/science/article/pii/S2405654524001446Weaned pigletMetasilicate-based alkaline mineral waterGut-liver axisSecondary bile acidsCholesterol metabolism |
| spellingShingle | Jian Chen Kanwar K. Malhi Xiaowei Li Xiangwen Xu Jianxun Kang Bichen Zhao Yaru Xu Xuenan Li Jinlong Li Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathway Animal Nutrition Weaned piglet Metasilicate-based alkaline mineral water Gut-liver axis Secondary bile acids Cholesterol metabolism |
| title | Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathway |
| title_full | Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathway |
| title_fullStr | Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathway |
| title_full_unstemmed | Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathway |
| title_short | Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathway |
| title_sort | metasilicate based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut liver axis homeostasis through microbiota mediated secondary bile acid pathway |
| topic | Weaned piglet Metasilicate-based alkaline mineral water Gut-liver axis Secondary bile acids Cholesterol metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2405654524001446 |
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