Genomic characteristics and molecular epidemiology of MRSA from medical centers in Mexico: Results from the Invifar network.
<h4>Introduction</h4>The methicillin-resistant Staphylococcus aureus (MRSA) genome varies by geographical location. This study aims to determine the genomic characteristics of MRSA using whole-genome sequencing (WGS) data from medical centers in Mexico and to explore the associations bet...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0317284 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832540189988225024 |
|---|---|
| author | Gabriela Echaniz-Avilés Luis Ángel Núñez-García Eduardo Rodríguez-Noriega Consuelo Velázquez-Acosta Luis Esaú López-Jácome Eduardo López-Gutiérrez Talia Pérez-Vicelis Cristina Torres-Báez Ulises Garza-Ramos Nadia Rodríguez-Medina Juan Manuel Barajas-Magallón Rosario Vázquez-Larios Aldo Rafael Silva-Gamiño Rafael Franco-Cendejas Adolfo Gómez-Quiroz Elvira Garza-González |
| author_facet | Gabriela Echaniz-Avilés Luis Ángel Núñez-García Eduardo Rodríguez-Noriega Consuelo Velázquez-Acosta Luis Esaú López-Jácome Eduardo López-Gutiérrez Talia Pérez-Vicelis Cristina Torres-Báez Ulises Garza-Ramos Nadia Rodríguez-Medina Juan Manuel Barajas-Magallón Rosario Vázquez-Larios Aldo Rafael Silva-Gamiño Rafael Franco-Cendejas Adolfo Gómez-Quiroz Elvira Garza-González |
| author_sort | Gabriela Echaniz-Avilés |
| collection | DOAJ |
| description | <h4>Introduction</h4>The methicillin-resistant Staphylococcus aureus (MRSA) genome varies by geographical location. This study aims to determine the genomic characteristics of MRSA using whole-genome sequencing (WGS) data from medical centers in Mexico and to explore the associations between antimicrobial resistance genes and virulence factors.<h4>Methods</h4>This study included 27 clinical isolates collected from sterile sites at eight centers in Mexico in 2022 and 2023. Antibiotic susceptibility testing was performed using VITEK 2. In addition, WGS was performed using a NovaSeq platform, and a bioinformatic analysis was conducted using several tools.<h4>Results</h4>In this study, 21 strains were CC5, five were CC8, and one was CC93. Moreover, six strains were identified as ST5(CC5)-MRSA-IIa- t895, four strains were found to be ST1011(CC5)-MRSA-IIa-t895, five strains were found to be ST1011(CC5)-MRSA-IIa-t9364, one strain was found to be ST1011(CC5)-MRSA-IIa-t8116, another was found to be ST1011(CC5)-MRSA-IIa-t62, three were found to be ST8(CC8)-MRSA-IVa-t8, one strain was ST5(CC5)-MRSA-IVa-t2, one strain was as ST93(CC93)-MRSA-IVa-t3949, two strains were ST9003(CC8)-MRSA-IVa-t18492, and three strains were ST9034(CC5)-MRSA-V-t2. All SCCmec IIa strains showed resistance to levofloxacin and ciprofloxacin, and all but two strains were resistant to clindamycin. Among the strains that harbored the type IIa cassette, most had the aadD, blaZ, and ermA_SDS genes and the erm A gene. Multiple genes for adhesion, enzymes, immune evasion, and secretion system were detected, regardless of SCCmec type. Of the SCCmec IVa strains, most harbored the Panton-Valentine leukocidin encoding genes.<h4>Conclusion</h4>In this study, the most frequently detected CC was CC5, followed by CC8, and CC93, and the most frequently detected MRSA ST was ST1011, followed by ST5. Most SCCmec elements were found to be type IIa, followed by type IVa. High MIC values were observed for ciprofloxacin, erythromycin, and clindamycin, particularly within SCCmec IIa. Of the SCCmec IVa strains, most harbored the lukS-PV and lukF-PV genes. |
| format | Article |
| id | doaj-art-778ed88b0ea74bf1a50ff1995db0c007 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-778ed88b0ea74bf1a50ff1995db0c0072025-02-05T05:32:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031728410.1371/journal.pone.0317284Genomic characteristics and molecular epidemiology of MRSA from medical centers in Mexico: Results from the Invifar network.Gabriela Echaniz-AvilésLuis Ángel Núñez-GarcíaEduardo Rodríguez-NoriegaConsuelo Velázquez-AcostaLuis Esaú López-JácomeEduardo López-GutiérrezTalia Pérez-VicelisCristina Torres-BáezUlises Garza-RamosNadia Rodríguez-MedinaJuan Manuel Barajas-MagallónRosario Vázquez-LariosAldo Rafael Silva-GamiñoRafael Franco-CendejasAdolfo Gómez-QuirozElvira Garza-González<h4>Introduction</h4>The methicillin-resistant Staphylococcus aureus (MRSA) genome varies by geographical location. This study aims to determine the genomic characteristics of MRSA using whole-genome sequencing (WGS) data from medical centers in Mexico and to explore the associations between antimicrobial resistance genes and virulence factors.<h4>Methods</h4>This study included 27 clinical isolates collected from sterile sites at eight centers in Mexico in 2022 and 2023. Antibiotic susceptibility testing was performed using VITEK 2. In addition, WGS was performed using a NovaSeq platform, and a bioinformatic analysis was conducted using several tools.<h4>Results</h4>In this study, 21 strains were CC5, five were CC8, and one was CC93. Moreover, six strains were identified as ST5(CC5)-MRSA-IIa- t895, four strains were found to be ST1011(CC5)-MRSA-IIa-t895, five strains were found to be ST1011(CC5)-MRSA-IIa-t9364, one strain was found to be ST1011(CC5)-MRSA-IIa-t8116, another was found to be ST1011(CC5)-MRSA-IIa-t62, three were found to be ST8(CC8)-MRSA-IVa-t8, one strain was ST5(CC5)-MRSA-IVa-t2, one strain was as ST93(CC93)-MRSA-IVa-t3949, two strains were ST9003(CC8)-MRSA-IVa-t18492, and three strains were ST9034(CC5)-MRSA-V-t2. All SCCmec IIa strains showed resistance to levofloxacin and ciprofloxacin, and all but two strains were resistant to clindamycin. Among the strains that harbored the type IIa cassette, most had the aadD, blaZ, and ermA_SDS genes and the erm A gene. Multiple genes for adhesion, enzymes, immune evasion, and secretion system were detected, regardless of SCCmec type. Of the SCCmec IVa strains, most harbored the Panton-Valentine leukocidin encoding genes.<h4>Conclusion</h4>In this study, the most frequently detected CC was CC5, followed by CC8, and CC93, and the most frequently detected MRSA ST was ST1011, followed by ST5. Most SCCmec elements were found to be type IIa, followed by type IVa. High MIC values were observed for ciprofloxacin, erythromycin, and clindamycin, particularly within SCCmec IIa. Of the SCCmec IVa strains, most harbored the lukS-PV and lukF-PV genes.https://doi.org/10.1371/journal.pone.0317284 |
| spellingShingle | Gabriela Echaniz-Avilés Luis Ángel Núñez-García Eduardo Rodríguez-Noriega Consuelo Velázquez-Acosta Luis Esaú López-Jácome Eduardo López-Gutiérrez Talia Pérez-Vicelis Cristina Torres-Báez Ulises Garza-Ramos Nadia Rodríguez-Medina Juan Manuel Barajas-Magallón Rosario Vázquez-Larios Aldo Rafael Silva-Gamiño Rafael Franco-Cendejas Adolfo Gómez-Quiroz Elvira Garza-González Genomic characteristics and molecular epidemiology of MRSA from medical centers in Mexico: Results from the Invifar network. PLoS ONE |
| title | Genomic characteristics and molecular epidemiology of MRSA from medical centers in Mexico: Results from the Invifar network. |
| title_full | Genomic characteristics and molecular epidemiology of MRSA from medical centers in Mexico: Results from the Invifar network. |
| title_fullStr | Genomic characteristics and molecular epidemiology of MRSA from medical centers in Mexico: Results from the Invifar network. |
| title_full_unstemmed | Genomic characteristics and molecular epidemiology of MRSA from medical centers in Mexico: Results from the Invifar network. |
| title_short | Genomic characteristics and molecular epidemiology of MRSA from medical centers in Mexico: Results from the Invifar network. |
| title_sort | genomic characteristics and molecular epidemiology of mrsa from medical centers in mexico results from the invifar network |
| url | https://doi.org/10.1371/journal.pone.0317284 |
| work_keys_str_mv | AT gabrielaechanizaviles genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT luisangelnunezgarcia genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT eduardorodrigueznoriega genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT consuelovelazquezacosta genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT luisesaulopezjacome genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT eduardolopezgutierrez genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT taliaperezvicelis genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT cristinatorresbaez genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT ulisesgarzaramos genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT nadiarodriguezmedina genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT juanmanuelbarajasmagallon genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT rosariovazquezlarios genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT aldorafaelsilvagamino genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT rafaelfrancocendejas genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT adolfogomezquiroz genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork AT elviragarzagonzalez genomiccharacteristicsandmolecularepidemiologyofmrsafrommedicalcentersinmexicoresultsfromtheinvifarnetwork |