Dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickens
ABSTRACT: Dermal hyperpigmentation stands out among the various skin pigmentation phenotypes in chickens, where most other pigmentation variants affect feather color and patterning predominantly. Despite numerous black chicken breeds worldwide, only a select few exhibit comprehensive black pigmentat...
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124007892 |
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| author | Dong Leng Maosen Yang Xiaomeng Miao Zhiying Huang Mengmeng Li Jia Liu Tao Wang Diyan Li Chungang Feng |
| author_facet | Dong Leng Maosen Yang Xiaomeng Miao Zhiying Huang Mengmeng Li Jia Liu Tao Wang Diyan Li Chungang Feng |
| author_sort | Dong Leng |
| collection | DOAJ |
| description | ABSTRACT: Dermal hyperpigmentation stands out among the various skin pigmentation phenotypes in chickens, where most other pigmentation variants affect feather color and patterning predominantly. Despite numerous black chicken breeds worldwide, only a select few exhibit comprehensive black pigmentation, which encompasses the skin, meat, flesh, and bones. The process of skin melanin pigmentation is intricate and develops successively. Historically, research has concentrated primarily on specific developmental points or stages, but fewer studies have examined the entire transcriptome across the timeline of the development of the embryo integument. In our investigation, we undertook the sequencing of chicken embryo skin samples from d 4 to d 13 of incubation. Our results showed that melanoblasts continued to migrate from E4 to the epidermis until E12. Beginning with E6, melanin was synthesized and transferred to epidermal cells and feather follicles in large quantities, and genes such as DCT, TYR, TYRP1, and MITF played a key role in this process, which is significantly different from that of white-skinned chickens. There were 854 differentially expressed genes between E7 and E8. At this stage, melanocytes formed dendritic forms and transferred melanin to keratinocytes, while the dorsal skin became visibly dark. In addition, CDH3, which is a core factor involved in a variety of biological processes, may have an important impact on skin melanin pigmentation. Collectively, our findings unveiled a phased relationship between the canonical pathway and the noncanonical pathway from E4 to E13. These analyses illuminated the gene regulatory mechanism and provided foundational data that pertained to pigmentation in chickens. |
| format | Article |
| id | doaj-art-775de39f9b204c69beaa299d1b61f1ac |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj-art-775de39f9b204c69beaa299d1b61f1ac2025-01-22T05:40:04ZengElsevierPoultry Science0032-57912025-01-011041104210Dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickensDong Leng0Maosen Yang1Xiaomeng Miao2Zhiying Huang3Mengmeng Li4Jia Liu5Tao Wang6Diyan Li7Chungang Feng8College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; School of Pharmacy, Chengdu University, Chengdu 610106, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China; School of Pharmacy, Chengdu University, Chengdu 610106, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang 550005, ChinaCollege of Animal Science, Shanxi Agricultural University, Taiyuan 030031, China; School of Pharmacy, Chengdu University, Chengdu 610106, ChinaSchool of Pharmacy, Chengdu University, Chengdu 610106, ChinaGuizhou Province Livestock and Poultry Genetic Resources Management Station, Guizhou Provincial Department of Agriculture and Rural Affairs, Guiyang 550001, ChinaSchool of Pharmacy, Chengdu University, Chengdu 610106, ChinaSchool of Pharmacy, Chengdu University, Chengdu 610106, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author:ABSTRACT: Dermal hyperpigmentation stands out among the various skin pigmentation phenotypes in chickens, where most other pigmentation variants affect feather color and patterning predominantly. Despite numerous black chicken breeds worldwide, only a select few exhibit comprehensive black pigmentation, which encompasses the skin, meat, flesh, and bones. The process of skin melanin pigmentation is intricate and develops successively. Historically, research has concentrated primarily on specific developmental points or stages, but fewer studies have examined the entire transcriptome across the timeline of the development of the embryo integument. In our investigation, we undertook the sequencing of chicken embryo skin samples from d 4 to d 13 of incubation. Our results showed that melanoblasts continued to migrate from E4 to the epidermis until E12. Beginning with E6, melanin was synthesized and transferred to epidermal cells and feather follicles in large quantities, and genes such as DCT, TYR, TYRP1, and MITF played a key role in this process, which is significantly different from that of white-skinned chickens. There were 854 differentially expressed genes between E7 and E8. At this stage, melanocytes formed dendritic forms and transferred melanin to keratinocytes, while the dorsal skin became visibly dark. In addition, CDH3, which is a core factor involved in a variety of biological processes, may have an important impact on skin melanin pigmentation. Collectively, our findings unveiled a phased relationship between the canonical pathway and the noncanonical pathway from E4 to E13. These analyses illuminated the gene regulatory mechanism and provided foundational data that pertained to pigmentation in chickens.http://www.sciencedirect.com/science/article/pii/S0032579124007892chickenskintranscriptomeembryomelanin pigmentation |
| spellingShingle | Dong Leng Maosen Yang Xiaomeng Miao Zhiying Huang Mengmeng Li Jia Liu Tao Wang Diyan Li Chungang Feng Dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickens Poultry Science chicken skin transcriptome embryo melanin pigmentation |
| title | Dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickens |
| title_full | Dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickens |
| title_fullStr | Dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickens |
| title_full_unstemmed | Dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickens |
| title_short | Dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickens |
| title_sort | dynamic changes in the skin transcriptome for the melanin pigmentation in embryonic chickens |
| topic | chicken skin transcriptome embryo melanin pigmentation |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124007892 |
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