ABA enhances the apoptotic effect of docetaxel in the multidrug-resistant DU145 prostate cancer cell line
This study aimed to induce drug resistance in DU145 prostate cancer cells by exposing them to docetaxel and mitoxantrone, and to examine the effects of combining docetaxel and abscisic acid (ABA). The IC50 values for docetaxel and mitoxantrone in non-resistant cells were 54.57 nM and 6.25 nM, respec...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2024-01-01
|
| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400031S.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832581962003382272 |
|---|---|
| author | Şumnulu Deniz Doğanlar Zeynep Banu |
| author_facet | Şumnulu Deniz Doğanlar Zeynep Banu |
| author_sort | Şumnulu Deniz |
| collection | DOAJ |
| description | This study aimed to induce drug resistance in DU145 prostate cancer cells by exposing them to docetaxel and mitoxantrone, and to examine the effects of combining docetaxel and abscisic acid (ABA). The IC50 values for docetaxel and mitoxantrone in non-resistant cells were 54.57 nM and 6.25 nM, respectively, rising to 808.53 nM and 50.07 nM after resistance had developed. RT-PCR analysis showed that treatment of resistant cells with 50.07 nM docetaxel and 500 μM ABA (ABA) resulted in the following changes in gene expression: heat shock protein (HSP) 70 (0.63-fold), glucose-regulated protein 94 (GRP94) 0.33-fold, inositol-requiring transmembrane kinase endoribonuclease-1α (IRE1α) 1.62-fold, ER degradation- enhancing alpha-mannosidase-like 1 (EDEM1) 1.77-fold, X-box binding protein 1 (XBP1) 1.53-fold, p21 (2.53-fold), cellular tumor antigen p53 (p53) 2.49-fold, bcl-2-like protein 4 (Bax) 2.7-fold, and tumor necrosis factor (TNF-α) 6.35-fold. Tali™ cytometry analysis showed a 47% increase in apoptotic/necrotic cells with the combined treatment of docetaxel and ABA, compared to a 26% increase with docetaxel alone. Fluorescent staining revealed that co-administration of docetaxel and ABA increases apoptosis in resistant DU145 cells compared to treatment with docetaxel alone. This study suggests that combining ABA with docetaxel could be effective in drug-resistant prostate cancer. |
| format | Article |
| id | doaj-art-773002ce672f40149c4becac9e53bde1 |
| institution | Kabale University |
| issn | 0354-4664 1821-4339 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj-art-773002ce672f40149c4becac9e53bde12025-01-30T06:38:39ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392024-01-0176440941810.2298/ABS240812031S0354-46642400031SABA enhances the apoptotic effect of docetaxel in the multidrug-resistant DU145 prostate cancer cell lineŞumnulu Deniz0https://orcid.org/0000-0002-0331-4035Doğanlar Zeynep Banu1https://orcid.org/0000-0002-1365-9897Technology Research Development Application and Research Centre, Trakya University, Edirne, TurkeyFaculty of Medicine, Department of Medical Biology, Trakya University, Edirne, TurkeyThis study aimed to induce drug resistance in DU145 prostate cancer cells by exposing them to docetaxel and mitoxantrone, and to examine the effects of combining docetaxel and abscisic acid (ABA). The IC50 values for docetaxel and mitoxantrone in non-resistant cells were 54.57 nM and 6.25 nM, respectively, rising to 808.53 nM and 50.07 nM after resistance had developed. RT-PCR analysis showed that treatment of resistant cells with 50.07 nM docetaxel and 500 μM ABA (ABA) resulted in the following changes in gene expression: heat shock protein (HSP) 70 (0.63-fold), glucose-regulated protein 94 (GRP94) 0.33-fold, inositol-requiring transmembrane kinase endoribonuclease-1α (IRE1α) 1.62-fold, ER degradation- enhancing alpha-mannosidase-like 1 (EDEM1) 1.77-fold, X-box binding protein 1 (XBP1) 1.53-fold, p21 (2.53-fold), cellular tumor antigen p53 (p53) 2.49-fold, bcl-2-like protein 4 (Bax) 2.7-fold, and tumor necrosis factor (TNF-α) 6.35-fold. Tali™ cytometry analysis showed a 47% increase in apoptotic/necrotic cells with the combined treatment of docetaxel and ABA, compared to a 26% increase with docetaxel alone. Fluorescent staining revealed that co-administration of docetaxel and ABA increases apoptosis in resistant DU145 cells compared to treatment with docetaxel alone. This study suggests that combining ABA with docetaxel could be effective in drug-resistant prostate cancer.https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400031S.pdfabadocetaxeldrug resistancehsp70grp94 |
| spellingShingle | Şumnulu Deniz Doğanlar Zeynep Banu ABA enhances the apoptotic effect of docetaxel in the multidrug-resistant DU145 prostate cancer cell line Archives of Biological Sciences aba docetaxel drug resistance hsp70 grp94 |
| title | ABA enhances the apoptotic effect of docetaxel in the multidrug-resistant DU145 prostate cancer cell line |
| title_full | ABA enhances the apoptotic effect of docetaxel in the multidrug-resistant DU145 prostate cancer cell line |
| title_fullStr | ABA enhances the apoptotic effect of docetaxel in the multidrug-resistant DU145 prostate cancer cell line |
| title_full_unstemmed | ABA enhances the apoptotic effect of docetaxel in the multidrug-resistant DU145 prostate cancer cell line |
| title_short | ABA enhances the apoptotic effect of docetaxel in the multidrug-resistant DU145 prostate cancer cell line |
| title_sort | aba enhances the apoptotic effect of docetaxel in the multidrug resistant du145 prostate cancer cell line |
| topic | aba docetaxel drug resistance hsp70 grp94 |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400031S.pdf |
| work_keys_str_mv | AT sumnuludeniz abaenhancestheapoptoticeffectofdocetaxelinthemultidrugresistantdu145prostatecancercellline AT doganlarzeynepbanu abaenhancestheapoptoticeffectofdocetaxelinthemultidrugresistantdu145prostatecancercellline |