Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS
The use of oral fluid as a biological matrix to monitor the use of drugs of abuse is a global trend because it presents several advantages and good correlation to the blood level. Thus, the present work aimed to develop and validate an analytical method for quantification and detection of solvents u...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/1029286 |
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| author | Bruna Claudia Coppe Bruna Tassi Borille Taís Regina Fiorentin Ana Laura Bemvenuti Jacques Ana Claudia Fagundes Stela Maris de Jezus Castro Lysa Silveira Remy Flavio Pechansky Renata Pereira Limberger |
| author_facet | Bruna Claudia Coppe Bruna Tassi Borille Taís Regina Fiorentin Ana Laura Bemvenuti Jacques Ana Claudia Fagundes Stela Maris de Jezus Castro Lysa Silveira Remy Flavio Pechansky Renata Pereira Limberger |
| author_sort | Bruna Claudia Coppe |
| collection | DOAJ |
| description | The use of oral fluid as a biological matrix to monitor the use of drugs of abuse is a global trend because it presents several advantages and good correlation to the blood level. Thus, the present work aimed to develop and validate an analytical method for quantification and detection of solvents used as inhalants of abuse in oral fluid (OF), using Quantisal™ as collector device by headspace and gas chromatography coupled with a mass detector (HS-GC/MS). Chromatographic separation was performed with a ZB-BAC1 column and the total time of analysis was 11.8 min. The method showed good linearity (correlation coefficient higher than 0.99 for all solvents). The limits of detection ranged from 0.05 to 5 mg/L, while the lower limits of quantification ranged from 2.5 to 12.5 mg/L. Accuracy, precision, matrix effect, and residual effect presented satisfactory results, meeting the criteria accepted for the validation of bioanalytical methods. The method showed good selectivity considering that, for solvents coeluting at the same retention time, resolution was performed by the mass detector. The method developed proved to be adequate when applied in OF samples from users of drugs and may be used to monitor the abuse of inhalants in routine forensic analyses. |
| format | Article |
| id | doaj-art-772b61cc33c24f82bd5c4fa90c6bdc61 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-772b61cc33c24f82bd5c4fa90c6bdc612025-02-03T01:31:05ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792016-01-01201610.1155/2016/10292861029286Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MSBruna Claudia Coppe0Bruna Tassi Borille1Taís Regina Fiorentin2Ana Laura Bemvenuti Jacques3Ana Claudia Fagundes4Stela Maris de Jezus Castro5Lysa Silveira Remy6Flavio Pechansky7Renata Pereira Limberger8Postgraduate Program in Pharmaceutical Sciences, School of Pharmacy, Federal University of Rio Grande do Sul, Avenida Ipiranga 2752, Santana, 90610-000 Porto Alegre, RS, BrazilPostgraduate Program in Pharmaceutical Sciences, School of Pharmacy, Federal University of Rio Grande do Sul, Avenida Ipiranga 2752, Santana, 90610-000 Porto Alegre, RS, BrazilPostgraduate Program in Pharmaceutical Sciences, School of Pharmacy, Federal University of Rio Grande do Sul, Avenida Ipiranga 2752, Santana, 90610-000 Porto Alegre, RS, BrazilPostgraduate Program in Pharmaceutical Sciences, School of Pharmacy, Federal University of Rio Grande do Sul, Avenida Ipiranga 2752, Santana, 90610-000 Porto Alegre, RS, BrazilPostgraduate Program in Pharmaceutical Sciences, School of Pharmacy, Federal University of Rio Grande do Sul, Avenida Ipiranga 2752, Santana, 90610-000 Porto Alegre, RS, BrazilDepartment of Statistics, Federal University of Rio Grande do Sul, Avenida Bento Gonçalves 9500, 91509-200 Porto Alegre, RS, BrazilCenter for Drug and Alcohol Research, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos 2350, Santana, 90035-903 Porto Alegre, RS, BrazilCenter for Drug and Alcohol Research, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos 2350, Santana, 90035-903 Porto Alegre, RS, BrazilPostgraduate Program in Pharmaceutical Sciences, School of Pharmacy, Federal University of Rio Grande do Sul, Avenida Ipiranga 2752, Santana, 90610-000 Porto Alegre, RS, BrazilThe use of oral fluid as a biological matrix to monitor the use of drugs of abuse is a global trend because it presents several advantages and good correlation to the blood level. Thus, the present work aimed to develop and validate an analytical method for quantification and detection of solvents used as inhalants of abuse in oral fluid (OF), using Quantisal™ as collector device by headspace and gas chromatography coupled with a mass detector (HS-GC/MS). Chromatographic separation was performed with a ZB-BAC1 column and the total time of analysis was 11.8 min. The method showed good linearity (correlation coefficient higher than 0.99 for all solvents). The limits of detection ranged from 0.05 to 5 mg/L, while the lower limits of quantification ranged from 2.5 to 12.5 mg/L. Accuracy, precision, matrix effect, and residual effect presented satisfactory results, meeting the criteria accepted for the validation of bioanalytical methods. The method showed good selectivity considering that, for solvents coeluting at the same retention time, resolution was performed by the mass detector. The method developed proved to be adequate when applied in OF samples from users of drugs and may be used to monitor the abuse of inhalants in routine forensic analyses.http://dx.doi.org/10.1155/2016/1029286 |
| spellingShingle | Bruna Claudia Coppe Bruna Tassi Borille Taís Regina Fiorentin Ana Laura Bemvenuti Jacques Ana Claudia Fagundes Stela Maris de Jezus Castro Lysa Silveira Remy Flavio Pechansky Renata Pereira Limberger Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS International Journal of Analytical Chemistry |
| title | Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
| title_full | Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
| title_fullStr | Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
| title_full_unstemmed | Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
| title_short | Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
| title_sort | multianalytical method validation for qualitative and quantitative analysis of solvents of abuse in oral fluid by hs gc ms |
| url | http://dx.doi.org/10.1155/2016/1029286 |
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