Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated Children
To control hepatitis B virus (HBV) infection, a universal HBV vaccination program for infants was launched in Taiwan in 1984. The aim of this study was to investigate the role of B-cell and T-cell epitope variations of HBsAg and polymerase in HBV infection in vaccinated children. One hundred sixty-t...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/571875 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557032199159808 |
|---|---|
| author | Yu-Min Lin Guey-Mei Jow Shu-Chi Mu Bing-Fang Chen |
| author_facet | Yu-Min Lin Guey-Mei Jow Shu-Chi Mu Bing-Fang Chen |
| author_sort | Yu-Min Lin |
| collection | DOAJ |
| description | To control hepatitis B virus (HBV) infection, a universal HBV vaccination program for infants was launched in Taiwan in 1984. The aim of this study was to investigate the role of B-cell and T-cell epitope variations of HBsAg and polymerase in HBV infection in vaccinated children. One hundred sixty-three sera from vaccinated children were enrolled randomly. HBV serum markers, including hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) and core antigen (anti-HBc), were detected by ELISA. Nucleotide sequences encoding the S and the pre-S regions of HBsAg were analyzed in all HBsAg positive sera. Five children were HBsAg positive. Sequence analysis of S, pre-S, and overlapped polymerase (P) genes showed that HBV isolates of HBsAg-positive vaccinees were variants; no G145R but G145A and other substitutions were found in the “a” determinant. Fifteen, six, and eight amino acid substitutions within B-cell and T-cell epitopes of S, pre-S, and P regions were detected, respectively. Several immune-epitope mutants, such as S45T/A, N131T, I194V, and S207N in S, were detected in all isolates. In conclusion, our results suggested that these naturally occurring immunoepitope mutants, which changed their immunogenicity leading to escape from immune response, might cause HBV infection. |
| format | Article |
| id | doaj-art-771f6130a7884f0f92176aaeff944243 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-771f6130a7884f0f92176aaeff9442432025-02-03T05:43:45ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/571875571875Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated ChildrenYu-Min Lin0Guey-Mei Jow1Shu-Chi Mu2Bing-Fang Chen3School of Medicine, Fu-Jen Catholic University, New Taipei City 24205, TaiwanSchool of Medicine, Fu-Jen Catholic University, New Taipei City 24205, TaiwanSchool of Medicine, Fu-Jen Catholic University, New Taipei City 24205, TaiwanSchool of Medicine, Fu-Jen Catholic University, New Taipei City 24205, TaiwanTo control hepatitis B virus (HBV) infection, a universal HBV vaccination program for infants was launched in Taiwan in 1984. The aim of this study was to investigate the role of B-cell and T-cell epitope variations of HBsAg and polymerase in HBV infection in vaccinated children. One hundred sixty-three sera from vaccinated children were enrolled randomly. HBV serum markers, including hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) and core antigen (anti-HBc), were detected by ELISA. Nucleotide sequences encoding the S and the pre-S regions of HBsAg were analyzed in all HBsAg positive sera. Five children were HBsAg positive. Sequence analysis of S, pre-S, and overlapped polymerase (P) genes showed that HBV isolates of HBsAg-positive vaccinees were variants; no G145R but G145A and other substitutions were found in the “a” determinant. Fifteen, six, and eight amino acid substitutions within B-cell and T-cell epitopes of S, pre-S, and P regions were detected, respectively. Several immune-epitope mutants, such as S45T/A, N131T, I194V, and S207N in S, were detected in all isolates. In conclusion, our results suggested that these naturally occurring immunoepitope mutants, which changed their immunogenicity leading to escape from immune response, might cause HBV infection.http://dx.doi.org/10.1155/2013/571875 |
| spellingShingle | Yu-Min Lin Guey-Mei Jow Shu-Chi Mu Bing-Fang Chen Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated Children The Scientific World Journal |
| title | Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated Children |
| title_full | Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated Children |
| title_fullStr | Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated Children |
| title_full_unstemmed | Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated Children |
| title_short | Naturally Occurring Hepatitis B Virus B-Cell and T-Cell Epitope Mutants in Hepatitis B Vaccinated Children |
| title_sort | naturally occurring hepatitis b virus b cell and t cell epitope mutants in hepatitis b vaccinated children |
| url | http://dx.doi.org/10.1155/2013/571875 |
| work_keys_str_mv | AT yuminlin naturallyoccurringhepatitisbvirusbcellandtcellepitopemutantsinhepatitisbvaccinatedchildren AT gueymeijow naturallyoccurringhepatitisbvirusbcellandtcellepitopemutantsinhepatitisbvaccinatedchildren AT shuchimu naturallyoccurringhepatitisbvirusbcellandtcellepitopemutantsinhepatitisbvaccinatedchildren AT bingfangchen naturallyoccurringhepatitisbvirusbcellandtcellepitopemutantsinhepatitisbvaccinatedchildren |