In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights
The current study investigated the in vitro degradability, in vitro gas production, methane (CH<sub>4</sub>) production, and ruminal bacterial community of kenaf plants cut at different heights (130, 160, 190, 220, and 250 cm). These samples were subjected to an in vitro batch culture sy...
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MDPI AG
2025-01-01
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| Series: | Veterinary Sciences |
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| author | Mengwei Li Faiz-ul Hassan Qian Lin Muhammad Adeel Arshad Muhammad Uzair Akhtar Lijuan Peng Chengjian Yang Xin Liang Jiaxiang Huang |
| author_facet | Mengwei Li Faiz-ul Hassan Qian Lin Muhammad Adeel Arshad Muhammad Uzair Akhtar Lijuan Peng Chengjian Yang Xin Liang Jiaxiang Huang |
| author_sort | Mengwei Li |
| collection | DOAJ |
| description | The current study investigated the in vitro degradability, in vitro gas production, methane (CH<sub>4</sub>) production, and ruminal bacterial community of kenaf plants cut at different heights (130, 160, 190, 220, and 250 cm). These samples were subjected to an in vitro batch culture system using buffalo rumen fluid to measure gas and CH<sub>4</sub> production at 3, 6, 9, 12, 24, 36, 48, and 72 h of incubation. Results reveal that crude protein (CP) concentration was the highest at the 220 cm height compared with the other heights. With the increase in height, gas and CH<sub>4</sub> production decreased. However, the CH<sub>4</sub> production at 190 cm was higher compared with the other plant heights. Dry matter degradation was higher at 190 cm and 220 cm, while ammonia-N and microbial CP were higher at the 220 cm height compared with the other heights. However, neutral detergent fiber degradation was the highest at the 130 cm height. Total volatile fatty acids, acetic acid, acetic acid/propane ratio, and pH value did not differ among the treatments, except for propionic acid, which was higher at the 130 cm and 160 cm heights. Overall, harvesting kenaf at plant heights of up to 220 cm was better in terms of its promising nutritional quality, improved dry matter degradation, and microbial CP contents. |
| format | Article |
| id | doaj-art-7716f076234542a39a9d1f927ab4565b |
| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-7716f076234542a39a9d1f927ab4565b2025-01-24T13:52:07ZengMDPI AGVeterinary Sciences2306-73812025-01-011215010.3390/vetsci12010050In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting HeightsMengwei Li0Faiz-ul Hassan1Qian Lin2Muhammad Adeel Arshad3Muhammad Uzair Akhtar4Lijuan Peng5Chengjian Yang6Xin Liang7Jiaxiang Huang8Guangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Guangxi Buffalo Research Institute, Nanning 530001, ChinaFaculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur 63100, PakistanInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaLaboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, ChinaFaculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur 63100, PakistanGuangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Guangxi Buffalo Research Institute, Nanning 530001, ChinaGuangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Guangxi Buffalo Research Institute, Nanning 530001, ChinaGuangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Guangxi Buffalo Research Institute, Nanning 530001, ChinaGuangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Guangxi Buffalo Research Institute, Nanning 530001, ChinaThe current study investigated the in vitro degradability, in vitro gas production, methane (CH<sub>4</sub>) production, and ruminal bacterial community of kenaf plants cut at different heights (130, 160, 190, 220, and 250 cm). These samples were subjected to an in vitro batch culture system using buffalo rumen fluid to measure gas and CH<sub>4</sub> production at 3, 6, 9, 12, 24, 36, 48, and 72 h of incubation. Results reveal that crude protein (CP) concentration was the highest at the 220 cm height compared with the other heights. With the increase in height, gas and CH<sub>4</sub> production decreased. However, the CH<sub>4</sub> production at 190 cm was higher compared with the other plant heights. Dry matter degradation was higher at 190 cm and 220 cm, while ammonia-N and microbial CP were higher at the 220 cm height compared with the other heights. However, neutral detergent fiber degradation was the highest at the 130 cm height. Total volatile fatty acids, acetic acid, acetic acid/propane ratio, and pH value did not differ among the treatments, except for propionic acid, which was higher at the 130 cm and 160 cm heights. Overall, harvesting kenaf at plant heights of up to 220 cm was better in terms of its promising nutritional quality, improved dry matter degradation, and microbial CP contents.https://www.mdpi.com/2306-7381/12/1/50kenafforagedigestibilityfermentation characteristicsmethanebacterial diversity |
| spellingShingle | Mengwei Li Faiz-ul Hassan Qian Lin Muhammad Adeel Arshad Muhammad Uzair Akhtar Lijuan Peng Chengjian Yang Xin Liang Jiaxiang Huang In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights Veterinary Sciences kenaf forage digestibility fermentation characteristics methane bacterial diversity |
| title | In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights |
| title_full | In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights |
| title_fullStr | In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights |
| title_full_unstemmed | In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights |
| title_short | In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights |
| title_sort | in vitro evaluation of ruminal digestibility fermentation characteristics and bacterial diversity of kenaf crop at various cutting heights |
| topic | kenaf forage digestibility fermentation characteristics methane bacterial diversity |
| url | https://www.mdpi.com/2306-7381/12/1/50 |
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