CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging
Aged tendon-derived stem/progenitor cells (TSPCs) lead to age-related tendon disorders and impair tendon healing. However, the underlying molecular mechanisms of TSPC aging remain largely unknown. Here, we investigated the role of connective tissue growth factor (CTGF) in TSPC aging. CTGF protein an...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/6257537 |
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| author | Yun-feng Rui Min-hao Chen Ying-juan Li Long-fei Xiao Peng Geng Pei Wang Zheng-yuan Xu Xuan-pu Zhang Guang-chun Dai |
| author_facet | Yun-feng Rui Min-hao Chen Ying-juan Li Long-fei Xiao Peng Geng Pei Wang Zheng-yuan Xu Xuan-pu Zhang Guang-chun Dai |
| author_sort | Yun-feng Rui |
| collection | DOAJ |
| description | Aged tendon-derived stem/progenitor cells (TSPCs) lead to age-related tendon disorders and impair tendon healing. However, the underlying molecular mechanisms of TSPC aging remain largely unknown. Here, we investigated the role of connective tissue growth factor (CTGF) in TSPC aging. CTGF protein and mRNA levels were markedly decreased in the aged TSPCs. Moreover, recombinant CTGF attenuates TSPC aging and restores the age-associated reduction of self-renewal and differentiation of TSPCs. In addition, cell cycle distribution of aged TSPCs was arrested in the G1/S phase while recombinant CTGF treatment promoted G1/S transition. Recombinant CTGF also rescued decreased levels of cyclin D1 and CDK4 and reduced p27kip1 expression in aged TSPCs. Our results demonstrated that CTGF plays a vital role in TSPC aging and might be a potential target for molecular therapy of age-related tendon disorders. |
| format | Article |
| id | doaj-art-770b5681ad0e4299b9fe0bc224083610 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-770b5681ad0e4299b9fe0bc2240836102025-02-03T06:01:30ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/62575376257537CTGF Attenuates Tendon-Derived Stem/Progenitor Cell AgingYun-feng Rui0Min-hao Chen1Ying-juan Li2Long-fei Xiao3Peng Geng4Pei Wang5Zheng-yuan Xu6Xuan-pu Zhang7Guang-chun Dai8Department of Orthopaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, ChinaDepartment of Orthopaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, ChinaChina Orthopedic Regenerative Medicine Group, Hangzhou, Zhejiang 310000, ChinaDepartment of Orthopaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, ChinaDepartment of Orthopaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaDepartment of Orthopaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, ChinaAged tendon-derived stem/progenitor cells (TSPCs) lead to age-related tendon disorders and impair tendon healing. However, the underlying molecular mechanisms of TSPC aging remain largely unknown. Here, we investigated the role of connective tissue growth factor (CTGF) in TSPC aging. CTGF protein and mRNA levels were markedly decreased in the aged TSPCs. Moreover, recombinant CTGF attenuates TSPC aging and restores the age-associated reduction of self-renewal and differentiation of TSPCs. In addition, cell cycle distribution of aged TSPCs was arrested in the G1/S phase while recombinant CTGF treatment promoted G1/S transition. Recombinant CTGF also rescued decreased levels of cyclin D1 and CDK4 and reduced p27kip1 expression in aged TSPCs. Our results demonstrated that CTGF plays a vital role in TSPC aging and might be a potential target for molecular therapy of age-related tendon disorders.http://dx.doi.org/10.1155/2019/6257537 |
| spellingShingle | Yun-feng Rui Min-hao Chen Ying-juan Li Long-fei Xiao Peng Geng Pei Wang Zheng-yuan Xu Xuan-pu Zhang Guang-chun Dai CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging Stem Cells International |
| title | CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging |
| title_full | CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging |
| title_fullStr | CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging |
| title_full_unstemmed | CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging |
| title_short | CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging |
| title_sort | ctgf attenuates tendon derived stem progenitor cell aging |
| url | http://dx.doi.org/10.1155/2019/6257537 |
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