Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells
Activin A is involved in inflammation. The present study was performed to clarify if lipopolysaccharide, a component of Gram-negative bacteria, stimulates activin A secretion from human amniotic epithelial cells and to determine if activin A plays a role in amnionitis. Fetal membranes were obtained...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2013/789012 |
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| author | Yumiko Abe Risa Marukawa Nami Tsuru Maki Sato Hiroko Matsuda Hisanobu Sadakata Takashi Kameda Takashi Minegishi |
| author_facet | Yumiko Abe Risa Marukawa Nami Tsuru Maki Sato Hiroko Matsuda Hisanobu Sadakata Takashi Kameda Takashi Minegishi |
| author_sort | Yumiko Abe |
| collection | DOAJ |
| description | Activin A is involved in inflammation. The present study was performed to clarify if lipopolysaccharide, a component of Gram-negative bacteria, stimulates activin A secretion from human amniotic epithelial cells and to determine if activin A plays a role in amnionitis. Fetal membranes were obtained during elective cesarean sections performed in full-term pregnancies of patients without systemic disease, signs of premature delivery, or fetal complications. Amniotic epithelial cells were isolated by trypsinization. The activin A concentrations in the culture media were measured by enzyme-linked immunosorbent assay, and cell proliferation was assessed by 5-bromo-2′-deoxyuridine incorporation. Amniotic epithelial cells secreted activin A in a cell density-dependent manner, and lipopolysaccharide (10 μg/mL) enhanced the secretion at each cell density. Lipopolysaccharide (10–50 μg/mL) also stimulated activin A secretion in a dose-dependent manner. Contrary to the effect of activin A secretion, lipopolysaccharide inhibited cell proliferation in amniotic epithelial cells. The present study suggests that lipopolysaccharide stimulation of activin A secretion may be a mechanism in the pathogenesis of amnionitis. |
| format | Article |
| id | doaj-art-76f4821175cc4da79ec03c6842e83a37 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-76f4821175cc4da79ec03c6842e83a372025-02-03T00:59:21ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/789012789012Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial CellsYumiko Abe0Risa Marukawa1Nami Tsuru2Maki Sato3Hiroko Matsuda4Hisanobu Sadakata5Takashi Kameda6Takashi Minegishi7Department of Laboratory Sciences, Graduate School of Health Sciences, Gunma University, 3-39-22 Showa, Maebashi, Gunma 371-8514, JapanKuki General Hospital, Kuki, Saitama 346-0021, JapanMiyazaki Prefectural Nobeoka Hospital, Nobeoka, Miyazaki 882-0835, JapanYokota Maternity Hospital, Maebashi, Gunma 371-0031, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Gunma University, 3-39-22 Showa, Maebashi, Gunma 371-8511, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Gunma University, 3-39-22 Showa, Maebashi, Gunma 371-8511, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Gunma University, 3-39-22 Showa, Maebashi, Gunma 371-8511, JapanDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Gunma University, 3-39-22 Showa, Maebashi, Gunma 371-8511, JapanActivin A is involved in inflammation. The present study was performed to clarify if lipopolysaccharide, a component of Gram-negative bacteria, stimulates activin A secretion from human amniotic epithelial cells and to determine if activin A plays a role in amnionitis. Fetal membranes were obtained during elective cesarean sections performed in full-term pregnancies of patients without systemic disease, signs of premature delivery, or fetal complications. Amniotic epithelial cells were isolated by trypsinization. The activin A concentrations in the culture media were measured by enzyme-linked immunosorbent assay, and cell proliferation was assessed by 5-bromo-2′-deoxyuridine incorporation. Amniotic epithelial cells secreted activin A in a cell density-dependent manner, and lipopolysaccharide (10 μg/mL) enhanced the secretion at each cell density. Lipopolysaccharide (10–50 μg/mL) also stimulated activin A secretion in a dose-dependent manner. Contrary to the effect of activin A secretion, lipopolysaccharide inhibited cell proliferation in amniotic epithelial cells. The present study suggests that lipopolysaccharide stimulation of activin A secretion may be a mechanism in the pathogenesis of amnionitis.http://dx.doi.org/10.1155/2013/789012 |
| spellingShingle | Yumiko Abe Risa Marukawa Nami Tsuru Maki Sato Hiroko Matsuda Hisanobu Sadakata Takashi Kameda Takashi Minegishi Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells International Journal of Endocrinology |
| title | Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells |
| title_full | Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells |
| title_fullStr | Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells |
| title_full_unstemmed | Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells |
| title_short | Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells |
| title_sort | gram negative bacterial lipopolysaccharide stimulates activin a secretion from human amniotic epithelial cells |
| url | http://dx.doi.org/10.1155/2013/789012 |
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