Genomic and resistome analysis of Salmonella enterica isolates from retail markets in Yichun city, China
Nontyphoidal Salmonella (NTS) causes global outbreaks of foodborne disease. The main source of Salmonella for humans is animal-borne foods; however, the monitoring of Salmonella in the food chain via genomic platforms was limited in China. This study evaluated the prevalence, resistome, and virulome...
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Elsevier
2025-06-01
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| author | Xiaowu Jiang Abubakar Siddique Li Chen Lexin Zhu Haiyang Zhou Li Na Chenghao Jia Yan Li Min Yue |
| author_facet | Xiaowu Jiang Abubakar Siddique Li Chen Lexin Zhu Haiyang Zhou Li Na Chenghao Jia Yan Li Min Yue |
| author_sort | Xiaowu Jiang |
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| description | Nontyphoidal Salmonella (NTS) causes global outbreaks of foodborne disease. The main source of Salmonella for humans is animal-borne foods; however, the monitoring of Salmonella in the food chain via genomic platforms was limited in China. This study evaluated the prevalence, resistome, and virulome diversity of Salmonella strains identified from pork, retail environment, aquatic products, and poultry eggs of retail markets in Yichun city, Jiangxi province. The overall incidence of Salmonella was 9.4 %, with a higher contamination rate observed in pork at 13.5 %, followed by the retail environment at 7.69 %. The genomic analysis of the isolates revealed a total of fifteen distinct serovars, with serovar Enteritidis being the most prevalent (64.3 %). The phenotypic resistance analysis conducted by the broth microdilution method, revealed that 81.12 % of the isolates exhibited multidrug resistance (MDR), with high resistance to trimethoprim/sulphonamides (100 %), followed by tetracycline (99.3 %) and streptomycin (99.3 %). Genotypic analysis of antimicrobial resistance identified 80 antimicrobial-resistant genes (ARGs), with mdf(A), aph(3′)-Ib, tet(A), dfrA12, floR, blaTEM-1B, qnrS3, and sul2, conferring resistance to different antimicrobial classes, being the predominant ARGs. Additionally, forty ESBL genes, particularly critical genes such as blaCTX-M and blaNDM-1, were also identified in Salmonella isolates. The IncR, IncFIB (K), and IncX1 plasmid replicons were widely prevalent and served as significant reservoirs of horizontally acquired foreign genes. Moreover, key virulence genes such as cdtB, lpf and sef were also detected, in addition to Salmonella pathogenicity islands SPI-1 and SPI-2. This study reveals the prevalence of multidrug-resistant and virulent strains of Salmonella serovars in the markets of Yichuan city, posing a risk of human infections. The gained knowledge provided essential baseline information that may be utilized for regular tracking of MDR Salmonella transmission in the food chain to minimize potential future outbreaks. |
| format | Article |
| id | doaj-art-76d7875bd1da49e9a34aaa1ea136bf65 |
| institution | Kabale University |
| issn | 2352-7714 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | One Health |
| spelling | doaj-art-76d7875bd1da49e9a34aaa1ea136bf652025-01-22T05:42:44ZengElsevierOne Health2352-77142025-06-0120100967Genomic and resistome analysis of Salmonella enterica isolates from retail markets in Yichun city, ChinaXiaowu Jiang0Abubakar Siddique1Li Chen2Lexin Zhu3Haiyang Zhou4Li Na5Chenghao Jia6Yan Li7Min Yue8College of Medicine, Yichun University, 576 Xuefu Road, Yichun, Jiangxi 336000, China; Jiangxi Provincial Key Laboratory of Active Component of Natural Drugs, Poster-Doctoral Research Center, 576 Xuefu Road, Yichun, Jiangxi 336000, ChinaInstitute of Preventive Veterinary Sciences and Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou 310058, China; Hainan Institute of Zhejiang University, Sanya 572000, ChinaCollege of Medicine, Yichun University, 576 Xuefu Road, Yichun, Jiangxi 336000, ChinaCollege of Medicine, Yichun University, 576 Xuefu Road, Yichun, Jiangxi 336000, ChinaInstitute of Preventive Veterinary Sciences and Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou 310058, China; Hainan Institute of Zhejiang University, Sanya 572000, ChinaInstitute of Preventive Veterinary Sciences and Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou 310058, ChinaInstitute of Preventive Veterinary Sciences and Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou 310058, China; Hainan Institute of Zhejiang University, Sanya 572000, ChinaInstitute of Preventive Veterinary Sciences and Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou 310058, China; Hainan Institute of Zhejiang University, Sanya 572000, ChinaInstitute of Preventive Veterinary Sciences and Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou 310058, China; Hainan Institute of Zhejiang University, Sanya 572000, China; Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou 310058, China; Corresponding author at: Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.Nontyphoidal Salmonella (NTS) causes global outbreaks of foodborne disease. The main source of Salmonella for humans is animal-borne foods; however, the monitoring of Salmonella in the food chain via genomic platforms was limited in China. This study evaluated the prevalence, resistome, and virulome diversity of Salmonella strains identified from pork, retail environment, aquatic products, and poultry eggs of retail markets in Yichun city, Jiangxi province. The overall incidence of Salmonella was 9.4 %, with a higher contamination rate observed in pork at 13.5 %, followed by the retail environment at 7.69 %. The genomic analysis of the isolates revealed a total of fifteen distinct serovars, with serovar Enteritidis being the most prevalent (64.3 %). The phenotypic resistance analysis conducted by the broth microdilution method, revealed that 81.12 % of the isolates exhibited multidrug resistance (MDR), with high resistance to trimethoprim/sulphonamides (100 %), followed by tetracycline (99.3 %) and streptomycin (99.3 %). Genotypic analysis of antimicrobial resistance identified 80 antimicrobial-resistant genes (ARGs), with mdf(A), aph(3′)-Ib, tet(A), dfrA12, floR, blaTEM-1B, qnrS3, and sul2, conferring resistance to different antimicrobial classes, being the predominant ARGs. Additionally, forty ESBL genes, particularly critical genes such as blaCTX-M and blaNDM-1, were also identified in Salmonella isolates. The IncR, IncFIB (K), and IncX1 plasmid replicons were widely prevalent and served as significant reservoirs of horizontally acquired foreign genes. Moreover, key virulence genes such as cdtB, lpf and sef were also detected, in addition to Salmonella pathogenicity islands SPI-1 and SPI-2. This study reveals the prevalence of multidrug-resistant and virulent strains of Salmonella serovars in the markets of Yichuan city, posing a risk of human infections. The gained knowledge provided essential baseline information that may be utilized for regular tracking of MDR Salmonella transmission in the food chain to minimize potential future outbreaks.http://www.sciencedirect.com/science/article/pii/S2352771425000035SalmonellaPorkRetail environmentResistomeOne health |
| spellingShingle | Xiaowu Jiang Abubakar Siddique Li Chen Lexin Zhu Haiyang Zhou Li Na Chenghao Jia Yan Li Min Yue Genomic and resistome analysis of Salmonella enterica isolates from retail markets in Yichun city, China One Health Salmonella Pork Retail environment Resistome One health |
| title | Genomic and resistome analysis of Salmonella enterica isolates from retail markets in Yichun city, China |
| title_full | Genomic and resistome analysis of Salmonella enterica isolates from retail markets in Yichun city, China |
| title_fullStr | Genomic and resistome analysis of Salmonella enterica isolates from retail markets in Yichun city, China |
| title_full_unstemmed | Genomic and resistome analysis of Salmonella enterica isolates from retail markets in Yichun city, China |
| title_short | Genomic and resistome analysis of Salmonella enterica isolates from retail markets in Yichun city, China |
| title_sort | genomic and resistome analysis of salmonella enterica isolates from retail markets in yichun city china |
| topic | Salmonella Pork Retail environment Resistome One health |
| url | http://www.sciencedirect.com/science/article/pii/S2352771425000035 |
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