The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity
Secretory Leukocyte Protease Inhibitor (SLPI) is a serine protease inhibitor produced by epithelial and myeloid cells with anti-inflammatory properties. Research has shown that SLPI exerts its anti-inflammatory activity by directly binding to NF-κB DNA binding sites and, in so doing, prevents bindin...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/507315 |
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| author | Niamh McGarry Catherine M. Greene Noel G. McElvaney Sinéad Weldon Clifford C. Taggart |
| author_facet | Niamh McGarry Catherine M. Greene Noel G. McElvaney Sinéad Weldon Clifford C. Taggart |
| author_sort | Niamh McGarry |
| collection | DOAJ |
| description | Secretory Leukocyte Protease Inhibitor (SLPI) is a serine protease inhibitor produced by epithelial and myeloid cells with anti-inflammatory properties. Research has shown that SLPI exerts its anti-inflammatory activity by directly binding to NF-κB DNA binding sites and, in so doing, prevents binding and subsequent transcription of proinflammatory gene expression. In the current study, we demonstrate that SLPI can inhibit TNF-α-induced apoptosis in U937 cells and peripheral blood monocytes. Specifically, SLPI inhibits TNF-α-induced caspase-3 activation and DNA degradation associated with apoptosis. We go on to show that this ability of SLPI to inhibit apoptosis is not dependent on its antiprotease activity as antiprotease deficient variants of SLPI can also inhibit TNF-α-induced apoptosis. This reduction in monocyte apoptosis may preserve monocyte function during inflammation resolution and promote infection clearance at mucosal sites. |
| format | Article |
| id | doaj-art-76a3fe115f1645548675b3c69a42bd57 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-76a3fe115f1645548675b3c69a42bd572025-02-03T05:58:05ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/507315507315The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease ActivityNiamh McGarry0Catherine M. Greene1Noel G. McElvaney2Sinéad Weldon3Clifford C. Taggart4Respiratory Research Division, Department of Medicine, Education and Research Centre, Royal College of Surgeons in Ireland, Dublin 9, IrelandRespiratory Research Division, Department of Medicine, Education and Research Centre, Royal College of Surgeons in Ireland, Dublin 9, IrelandRespiratory Research Division, Department of Medicine, Education and Research Centre, Royal College of Surgeons in Ireland, Dublin 9, IrelandCentre for Infection and Immunity, School of Medicine, Dentistry and Biomedical Sciences, Health Sciences Building, Queen’s University Belfast, 97 Lisburn Road, Belfast BT9 7AE, UKCentre for Infection and Immunity, School of Medicine, Dentistry and Biomedical Sciences, Health Sciences Building, Queen’s University Belfast, 97 Lisburn Road, Belfast BT9 7AE, UKSecretory Leukocyte Protease Inhibitor (SLPI) is a serine protease inhibitor produced by epithelial and myeloid cells with anti-inflammatory properties. Research has shown that SLPI exerts its anti-inflammatory activity by directly binding to NF-κB DNA binding sites and, in so doing, prevents binding and subsequent transcription of proinflammatory gene expression. In the current study, we demonstrate that SLPI can inhibit TNF-α-induced apoptosis in U937 cells and peripheral blood monocytes. Specifically, SLPI inhibits TNF-α-induced caspase-3 activation and DNA degradation associated with apoptosis. We go on to show that this ability of SLPI to inhibit apoptosis is not dependent on its antiprotease activity as antiprotease deficient variants of SLPI can also inhibit TNF-α-induced apoptosis. This reduction in monocyte apoptosis may preserve monocyte function during inflammation resolution and promote infection clearance at mucosal sites.http://dx.doi.org/10.1155/2015/507315 |
| spellingShingle | Niamh McGarry Catherine M. Greene Noel G. McElvaney Sinéad Weldon Clifford C. Taggart The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity Journal of Immunology Research |
| title | The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity |
| title_full | The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity |
| title_fullStr | The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity |
| title_full_unstemmed | The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity |
| title_short | The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity |
| title_sort | ability of secretory leukocyte protease inhibitor to inhibit apoptosis in monocytes is independent of its antiprotease activity |
| url | http://dx.doi.org/10.1155/2015/507315 |
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