Formalin Fixation as Tissue Preprocessing for Multimodal Optical Spectroscopy Using the Example of Human Brain Tumour Cross Sections
Characterization of brain tumours requires neuropathological expertise and is generally performed by histological evaluation and molecular analysis. One emerging technique to assist pathologists in future tumour diagnostics is multimodal optical spectroscopy. In the current clinical routine, tissue...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2021/5598309 |
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| author | Mona Stefanakis Anita Lorenz Jörg W. Bartsch Miriam C. Bassler Alexandra Wagner Marc Brecht Axel Pagenstecher Jens Schittenhelm Barbara Boldrini Sabrina Hakelberg Susan Noell Christopher Nimsky Marcos Tatagiba Rainer Ritz Karsten Rebner Edwin Ostertag |
| author_facet | Mona Stefanakis Anita Lorenz Jörg W. Bartsch Miriam C. Bassler Alexandra Wagner Marc Brecht Axel Pagenstecher Jens Schittenhelm Barbara Boldrini Sabrina Hakelberg Susan Noell Christopher Nimsky Marcos Tatagiba Rainer Ritz Karsten Rebner Edwin Ostertag |
| author_sort | Mona Stefanakis |
| collection | DOAJ |
| description | Characterization of brain tumours requires neuropathological expertise and is generally performed by histological evaluation and molecular analysis. One emerging technique to assist pathologists in future tumour diagnostics is multimodal optical spectroscopy. In the current clinical routine, tissue preprocessing with formalin is widely established and suitable for spectroscopic investigations since degradation processes impede the measurement of native tissue. However, formalin fixation results in alterations of the tissue chemistry and morphology for example by protein cross-linking. As optical spectroscopy is sensitive to these variations, we evaluate the effects of formalin fixation on multimodal brain tumour data in this proof-of-concept study. Nonfixed and formalin-fixed cross sections of different common human brain tumours were subjected to analysis of chemical variations using ultraviolet and Fourier-transform infrared microspectroscopy. Morphological changes were assessed by elastic light scattering microspectroscopy in the visible wavelength range. Data were analysed with multivariate data analysis and compared with histopathology. Tissue type classifications deduced by optical spectroscopy are highly comparable and independent from the preparation and the fixation protocol. However, formalin fixation leads to slightly better classification models due to improved stability of the tissue. As a consequence, spectroscopic methods represent an appropriate additional contrast for chemical and morphological information in neuropathological diagnosis and should be investigated to a greater extent. Furthermore, they can be included in the clinical workflow even after formalin fixation. |
| format | Article |
| id | doaj-art-769b928420a34d7fba091c4692f0d662 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-769b928420a34d7fba091c4692f0d6622025-02-03T06:43:48ZengWileyJournal of Spectroscopy2314-49202314-49392021-01-01202110.1155/2021/55983095598309Formalin Fixation as Tissue Preprocessing for Multimodal Optical Spectroscopy Using the Example of Human Brain Tumour Cross SectionsMona Stefanakis0Anita Lorenz1Jörg W. Bartsch2Miriam C. Bassler3Alexandra Wagner4Marc Brecht5Axel Pagenstecher6Jens Schittenhelm7Barbara Boldrini8Sabrina Hakelberg9Susan Noell10Christopher Nimsky11Marcos Tatagiba12Rainer Ritz13Karsten Rebner14Edwin Ostertag15Reutlingen University, Process Analysis and Technology PA & T, Alteburgstraße 150, Reutlingen 72762, GermanyReutlingen University, Process Analysis and Technology PA & T, Alteburgstraße 150, Reutlingen 72762, GermanyDepartment of Neurosurgery, Philipps University Marburg, Baldingerstraße, Marburg 35033, GermanyReutlingen University, Process Analysis and Technology PA & T, Alteburgstraße 150, Reutlingen 72762, GermanyReutlingen University, Process Analysis and Technology PA & T, Alteburgstraße 150, Reutlingen 72762, GermanyReutlingen University, Process Analysis and Technology PA & T, Alteburgstraße 150, Reutlingen 72762, GermanyDepartment of Neuropathology, Philipps University Marburg, Baldingerstraße, 35033 Marburg, GermanyInstitut Für Pathologie und Neuropathologie des Universitätsklinikums Tübingen und Zentrum für Neuroonkologie, Comprehensive Cancer Center Tübingen-Stuttgart, Tübingen University Hospital, Calwerstraße 3, Tübingen 72076, GermanyReutlingen University, Process Analysis and Technology PA & T, Alteburgstraße 150, Reutlingen 72762, GermanyDeutsche METROHM Prozessanalytik GmbH & Co. KG, In den Birken 1, Filderstadt 70794, GermanyDepartment of Neurosurgery, Tübingen University Hospital, Hoppe-Seyler-Straße 3, Tübingen 72076, GermanyDepartment of Neurosurgery, Philipps University Marburg, Baldingerstraße, Marburg 35033, GermanyDepartment of Neurosurgery, Tübingen University Hospital, Hoppe-Seyler-Straße 3, Tübingen 72076, GermanyDepartment of Neurosurgery, Philipps University Marburg, Baldingerstraße, Marburg 35033, GermanyReutlingen University, Process Analysis and Technology PA & T, Alteburgstraße 150, Reutlingen 72762, GermanyReutlingen University, Process Analysis and Technology PA & T, Alteburgstraße 150, Reutlingen 72762, GermanyCharacterization of brain tumours requires neuropathological expertise and is generally performed by histological evaluation and molecular analysis. One emerging technique to assist pathologists in future tumour diagnostics is multimodal optical spectroscopy. In the current clinical routine, tissue preprocessing with formalin is widely established and suitable for spectroscopic investigations since degradation processes impede the measurement of native tissue. However, formalin fixation results in alterations of the tissue chemistry and morphology for example by protein cross-linking. As optical spectroscopy is sensitive to these variations, we evaluate the effects of formalin fixation on multimodal brain tumour data in this proof-of-concept study. Nonfixed and formalin-fixed cross sections of different common human brain tumours were subjected to analysis of chemical variations using ultraviolet and Fourier-transform infrared microspectroscopy. Morphological changes were assessed by elastic light scattering microspectroscopy in the visible wavelength range. Data were analysed with multivariate data analysis and compared with histopathology. Tissue type classifications deduced by optical spectroscopy are highly comparable and independent from the preparation and the fixation protocol. However, formalin fixation leads to slightly better classification models due to improved stability of the tissue. As a consequence, spectroscopic methods represent an appropriate additional contrast for chemical and morphological information in neuropathological diagnosis and should be investigated to a greater extent. Furthermore, they can be included in the clinical workflow even after formalin fixation.http://dx.doi.org/10.1155/2021/5598309 |
| spellingShingle | Mona Stefanakis Anita Lorenz Jörg W. Bartsch Miriam C. Bassler Alexandra Wagner Marc Brecht Axel Pagenstecher Jens Schittenhelm Barbara Boldrini Sabrina Hakelberg Susan Noell Christopher Nimsky Marcos Tatagiba Rainer Ritz Karsten Rebner Edwin Ostertag Formalin Fixation as Tissue Preprocessing for Multimodal Optical Spectroscopy Using the Example of Human Brain Tumour Cross Sections Journal of Spectroscopy |
| title | Formalin Fixation as Tissue Preprocessing for Multimodal Optical Spectroscopy Using the Example of Human Brain Tumour Cross Sections |
| title_full | Formalin Fixation as Tissue Preprocessing for Multimodal Optical Spectroscopy Using the Example of Human Brain Tumour Cross Sections |
| title_fullStr | Formalin Fixation as Tissue Preprocessing for Multimodal Optical Spectroscopy Using the Example of Human Brain Tumour Cross Sections |
| title_full_unstemmed | Formalin Fixation as Tissue Preprocessing for Multimodal Optical Spectroscopy Using the Example of Human Brain Tumour Cross Sections |
| title_short | Formalin Fixation as Tissue Preprocessing for Multimodal Optical Spectroscopy Using the Example of Human Brain Tumour Cross Sections |
| title_sort | formalin fixation as tissue preprocessing for multimodal optical spectroscopy using the example of human brain tumour cross sections |
| url | http://dx.doi.org/10.1155/2021/5598309 |
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