Sempervirine inhibits proliferation, invasion and metastasis of ovarian cancer cells and induces ultrastructural changes in vivo
Abstract Ovarian cancer is one of the deadliest gynecological malignancies due to its late diagnosis and easy recurrence. Therefore, it is urgent to develop novel therapeutics for ovarian cancer treatment. In this study, we evaluated the anti-ovarian cancer effects of sempervirine in vitro and in vi...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Journal of Ovarian Research |
| Online Access: | https://doi.org/10.1186/s13048-024-01580-4 |
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| author | Danni Chen Yan Tan Tingting Chen Qin Wang Yan Yan Xiaoya Zhao Zhongxiao Zhang Jin Qiu Jian Zhang |
| author_facet | Danni Chen Yan Tan Tingting Chen Qin Wang Yan Yan Xiaoya Zhao Zhongxiao Zhang Jin Qiu Jian Zhang |
| author_sort | Danni Chen |
| collection | DOAJ |
| description | Abstract Ovarian cancer is one of the deadliest gynecological malignancies due to its late diagnosis and easy recurrence. Therefore, it is urgent to develop novel therapeutics for ovarian cancer treatment. In this study, we evaluated the anti-ovarian cancer effects of sempervirine in vitro and in vivo. CCK8 assays showed that sempervirine dose-dependently inhibited the proliferation of SKOV3 ovarian cancer cells. Transwell assays demonstrated that sempervirine significantly suppressed the invasion and metastasis of SKOV3 cells. Furthermore, in an orthotopic ovarian cancer mouse model, sempervirine dramatically inhibited tumor growth and induced pathological changes in tumor tissues, including poor development of tumor mucosa, collagen deposition, endoplasmic reticulum damage, mitochondrial swelling and vacuolar degeneration, which were similar to the positive control 5-Fu. Mechanistic studies revealed that sempervirine decreased the expression of proteins related to apelin signaling pathway. In conclusion, our results demonstrate the potent anti-ovarian cancer effects of sempervirine both in vitro and in vivo. Sempervirine may repress ovarian cancer by down-regulating apelin signaling pathway. Our study suggests that sempervirine is a promising therapeutic agent against ovarian cancer. |
| format | Article |
| id | doaj-art-7689b54489e24b15a19b2f1fe3fac759 |
| institution | Kabale University |
| issn | 1757-2215 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj-art-7689b54489e24b15a19b2f1fe3fac7592025-02-02T12:37:00ZengBMCJournal of Ovarian Research1757-22152025-01-0118111110.1186/s13048-024-01580-4Sempervirine inhibits proliferation, invasion and metastasis of ovarian cancer cells and induces ultrastructural changes in vivoDanni Chen0Yan Tan1Tingting Chen2Qin Wang3Yan Yan4Xiaoya Zhao5Zhongxiao Zhang6Jin Qiu7Jian Zhang8Department of Obstetrics and Gynecology, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong UniversityAbstract Ovarian cancer is one of the deadliest gynecological malignancies due to its late diagnosis and easy recurrence. Therefore, it is urgent to develop novel therapeutics for ovarian cancer treatment. In this study, we evaluated the anti-ovarian cancer effects of sempervirine in vitro and in vivo. CCK8 assays showed that sempervirine dose-dependently inhibited the proliferation of SKOV3 ovarian cancer cells. Transwell assays demonstrated that sempervirine significantly suppressed the invasion and metastasis of SKOV3 cells. Furthermore, in an orthotopic ovarian cancer mouse model, sempervirine dramatically inhibited tumor growth and induced pathological changes in tumor tissues, including poor development of tumor mucosa, collagen deposition, endoplasmic reticulum damage, mitochondrial swelling and vacuolar degeneration, which were similar to the positive control 5-Fu. Mechanistic studies revealed that sempervirine decreased the expression of proteins related to apelin signaling pathway. In conclusion, our results demonstrate the potent anti-ovarian cancer effects of sempervirine both in vitro and in vivo. Sempervirine may repress ovarian cancer by down-regulating apelin signaling pathway. Our study suggests that sempervirine is a promising therapeutic agent against ovarian cancer.https://doi.org/10.1186/s13048-024-01580-4 |
| spellingShingle | Danni Chen Yan Tan Tingting Chen Qin Wang Yan Yan Xiaoya Zhao Zhongxiao Zhang Jin Qiu Jian Zhang Sempervirine inhibits proliferation, invasion and metastasis of ovarian cancer cells and induces ultrastructural changes in vivo Journal of Ovarian Research |
| title | Sempervirine inhibits proliferation, invasion and metastasis of ovarian cancer cells and induces ultrastructural changes in vivo |
| title_full | Sempervirine inhibits proliferation, invasion and metastasis of ovarian cancer cells and induces ultrastructural changes in vivo |
| title_fullStr | Sempervirine inhibits proliferation, invasion and metastasis of ovarian cancer cells and induces ultrastructural changes in vivo |
| title_full_unstemmed | Sempervirine inhibits proliferation, invasion and metastasis of ovarian cancer cells and induces ultrastructural changes in vivo |
| title_short | Sempervirine inhibits proliferation, invasion and metastasis of ovarian cancer cells and induces ultrastructural changes in vivo |
| title_sort | sempervirine inhibits proliferation invasion and metastasis of ovarian cancer cells and induces ultrastructural changes in vivo |
| url | https://doi.org/10.1186/s13048-024-01580-4 |
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