Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury
The paracrine effect is the major mechanism of stem cell therapy. However, the details of the effect’s mechanism remain unknown. The aim of this study is to investigate whether adipose tissue-derived stem cells (ADSCs) can ameliorate cavernous nerve injury-induced erectile dysfunction (CNIED) rats a...
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| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/5161248 |
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| author | Xin Chen Qiyun Yang Tao Zheng Jun Bian Xiangzhou Sun Yanan Shi Xiaoyan Liang Guoquan Gao Guihua Liu Chunhua Deng |
| author_facet | Xin Chen Qiyun Yang Tao Zheng Jun Bian Xiangzhou Sun Yanan Shi Xiaoyan Liang Guoquan Gao Guihua Liu Chunhua Deng |
| author_sort | Xin Chen |
| collection | DOAJ |
| description | The paracrine effect is the major mechanism of stem cell therapy. However, the details of the effect’s mechanism remain unknown. The aim of this study is to investigate whether adipose tissue-derived stem cells (ADSCs) can ameliorate cavernous nerve injury-induced erectile dysfunction (CNIED) rats and to determine its mechanism. Twenty-eight days after intracavernous injection of 5-ethynyl-2-deoxyuridine- (EdU-) labeled ADSCs, the erectile function of all the rats was evaluated by intracavernosal pressure (ICP). The ADSCs steadily secreted detectable pigment epithelium-derived factor (PEDF) in vitro. The expression of PEDF increased in the penis of the bilateral cavernous nerve injury (BCNI) group for 14 days and then gradually decreased. On day 28 after the intracavernous injection, the ADSCs group exhibited a significantly increased ICP compared with the phosphate buffered saline- (PBS-) treated group. Moreover, the neuronal nitric oxide synthase (nNOS) and S100 expression in penile dorsal nerves and the smooth muscle content to collagen ratio in penile tissues significantly increased. Furthermore, elevated PEDF, p-Akt, and p-eNOS were identified in the ADSCs group. This study demonstrated that intracavernous injection of ADSCs improved erectile function, repaired the nerve, and corrected penile fibrosis. One potential mechanism is the PEDF secretion of ADSCs and subsequent PI3K/Akt pathway activation. |
| format | Article |
| id | doaj-art-767dc7937dfe4a2398acc83d379f6ef7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | Stem Cells International |
| spelling | doaj-art-767dc7937dfe4a2398acc83d379f6ef72025-02-03T05:45:07ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/51612485161248Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve InjuryXin Chen0Qiyun Yang1Tao Zheng2Jun Bian3Xiangzhou Sun4Yanan Shi5Xiaoyan Liang6Guoquan Gao7Guihua Liu8Chunhua Deng9Department of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, ChinaDepartment of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, ChinaReproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, ChinaReproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, ChinaDepartment of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaReproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, ChinaDepartment of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, ChinaThe paracrine effect is the major mechanism of stem cell therapy. However, the details of the effect’s mechanism remain unknown. The aim of this study is to investigate whether adipose tissue-derived stem cells (ADSCs) can ameliorate cavernous nerve injury-induced erectile dysfunction (CNIED) rats and to determine its mechanism. Twenty-eight days after intracavernous injection of 5-ethynyl-2-deoxyuridine- (EdU-) labeled ADSCs, the erectile function of all the rats was evaluated by intracavernosal pressure (ICP). The ADSCs steadily secreted detectable pigment epithelium-derived factor (PEDF) in vitro. The expression of PEDF increased in the penis of the bilateral cavernous nerve injury (BCNI) group for 14 days and then gradually decreased. On day 28 after the intracavernous injection, the ADSCs group exhibited a significantly increased ICP compared with the phosphate buffered saline- (PBS-) treated group. Moreover, the neuronal nitric oxide synthase (nNOS) and S100 expression in penile dorsal nerves and the smooth muscle content to collagen ratio in penile tissues significantly increased. Furthermore, elevated PEDF, p-Akt, and p-eNOS were identified in the ADSCs group. This study demonstrated that intracavernous injection of ADSCs improved erectile function, repaired the nerve, and corrected penile fibrosis. One potential mechanism is the PEDF secretion of ADSCs and subsequent PI3K/Akt pathway activation.http://dx.doi.org/10.1155/2016/5161248 |
| spellingShingle | Xin Chen Qiyun Yang Tao Zheng Jun Bian Xiangzhou Sun Yanan Shi Xiaoyan Liang Guoquan Gao Guihua Liu Chunhua Deng Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury Stem Cells International |
| title | Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury |
| title_full | Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury |
| title_fullStr | Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury |
| title_full_unstemmed | Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury |
| title_short | Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury |
| title_sort | neurotrophic effect of adipose tissue derived stem cells on erectile function recovery by pigment epithelium derived factor secretion in a rat model of cavernous nerve injury |
| url | http://dx.doi.org/10.1155/2016/5161248 |
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