Enhanced Production of Xylitol from Corncob by Pachysolen tannophilus Using Response Surface Methodology
Optimization of the culture medium and process variables for xylitol production using corncob hemicellulose hydrolysate by Pachysolen tannophilus (MTTC 1077) was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on xylitol produ...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Food Science |
| Online Access: | http://dx.doi.org/10.1155/2013/514676 |
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| author | S. Ramesh R. Muthuvelayudham R. Rajesh Kannan T. Viruthagiri |
| author_facet | S. Ramesh R. Muthuvelayudham R. Rajesh Kannan T. Viruthagiri |
| author_sort | S. Ramesh |
| collection | DOAJ |
| description | Optimization of the culture medium and process variables for xylitol production using corncob hemicellulose hydrolysate by Pachysolen tannophilus (MTTC 1077) was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on xylitol production was achieved using a Plackett-Burman design. Peptone, xylose, MgSO4·7H2O, and yeast extract were selected based on their positive influence on xylitol production. The selected components were optimized with Box-Behnken design using response surface methodology (RSM). The optimum levels (g/L) were peptone: 6.03, xylose: 10.62, MgSO4·7H2O: 1.39, yeast extract: 4.66. The influence of various process variables on the xylitol production was evaluated. The optimal levels of these variables were quantified by the central composite design using RSM, for establishment of a significant mathematical model with a coefficient determination of . The validation experimental was consistent with the prediction model. The optimum levels of process variables were temperature (36.56°C), pH (7.27), substrate concentration (3.55 g/L), inoculum size (3.69 mL), and agitation speed (194.44 rpm). These conditions were validated experimentally which revealed an enhanced xylitol yield of 0.80 g/g. |
| format | Article |
| id | doaj-art-766816e76ea94246a2d5072fc8e8e86c |
| institution | Kabale University |
| issn | 2314-5765 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Food Science |
| spelling | doaj-art-766816e76ea94246a2d5072fc8e8e86c2025-02-03T05:45:38ZengWileyInternational Journal of Food Science2314-57652013-01-01201310.1155/2013/514676514676Enhanced Production of Xylitol from Corncob by Pachysolen tannophilus Using Response Surface MethodologyS. Ramesh0R. Muthuvelayudham1R. Rajesh Kannan2T. Viruthagiri3Department of Chemical Engineering, Annamalai University, Annamalainagar 608002, Tamil Nadu, IndiaDepartment of Chemical Engineering, Annamalai University, Annamalainagar 608002, Tamil Nadu, IndiaDepartment of Chemical Engineering, Annamalai University, Annamalainagar 608002, Tamil Nadu, IndiaDepartment of Chemical Engineering, Annamalai University, Annamalainagar 608002, Tamil Nadu, IndiaOptimization of the culture medium and process variables for xylitol production using corncob hemicellulose hydrolysate by Pachysolen tannophilus (MTTC 1077) was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on xylitol production was achieved using a Plackett-Burman design. Peptone, xylose, MgSO4·7H2O, and yeast extract were selected based on their positive influence on xylitol production. The selected components were optimized with Box-Behnken design using response surface methodology (RSM). The optimum levels (g/L) were peptone: 6.03, xylose: 10.62, MgSO4·7H2O: 1.39, yeast extract: 4.66. The influence of various process variables on the xylitol production was evaluated. The optimal levels of these variables were quantified by the central composite design using RSM, for establishment of a significant mathematical model with a coefficient determination of . The validation experimental was consistent with the prediction model. The optimum levels of process variables were temperature (36.56°C), pH (7.27), substrate concentration (3.55 g/L), inoculum size (3.69 mL), and agitation speed (194.44 rpm). These conditions were validated experimentally which revealed an enhanced xylitol yield of 0.80 g/g.http://dx.doi.org/10.1155/2013/514676 |
| spellingShingle | S. Ramesh R. Muthuvelayudham R. Rajesh Kannan T. Viruthagiri Enhanced Production of Xylitol from Corncob by Pachysolen tannophilus Using Response Surface Methodology International Journal of Food Science |
| title | Enhanced Production of Xylitol from Corncob by Pachysolen tannophilus Using Response Surface Methodology |
| title_full | Enhanced Production of Xylitol from Corncob by Pachysolen tannophilus Using Response Surface Methodology |
| title_fullStr | Enhanced Production of Xylitol from Corncob by Pachysolen tannophilus Using Response Surface Methodology |
| title_full_unstemmed | Enhanced Production of Xylitol from Corncob by Pachysolen tannophilus Using Response Surface Methodology |
| title_short | Enhanced Production of Xylitol from Corncob by Pachysolen tannophilus Using Response Surface Methodology |
| title_sort | enhanced production of xylitol from corncob by pachysolen tannophilus using response surface methodology |
| url | http://dx.doi.org/10.1155/2013/514676 |
| work_keys_str_mv | AT sramesh enhancedproductionofxylitolfromcorncobbypachysolentannophilususingresponsesurfacemethodology AT rmuthuvelayudham enhancedproductionofxylitolfromcorncobbypachysolentannophilususingresponsesurfacemethodology AT rrajeshkannan enhancedproductionofxylitolfromcorncobbypachysolentannophilususingresponsesurfacemethodology AT tviruthagiri enhancedproductionofxylitolfromcorncobbypachysolentannophilususingresponsesurfacemethodology |