Response of the gut microbiome and metabolome to dietary fiber in healthy dogs
ABSTRACT Dietary fiber confers multiple health benefits originating from the expansion of beneficial gut microbial activity. However, very few studies have established the metabolic consequences of interactions among specific fibers, microbiome composition, and function in either human or representa...
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| Language: | English |
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American Society for Microbiology
2025-01-01
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| Series: | mSystems |
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| Online Access: | https://journals.asm.org/doi/10.1128/msystems.00452-24 |
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| author | Amrisha Bhosle Matthew I. Jackson Aaron M. Walsh Eric A. Franzosa Dayakar V. Badri Curtis Huttenhower |
| author_facet | Amrisha Bhosle Matthew I. Jackson Aaron M. Walsh Eric A. Franzosa Dayakar V. Badri Curtis Huttenhower |
| author_sort | Amrisha Bhosle |
| collection | DOAJ |
| description | ABSTRACT Dietary fiber confers multiple health benefits originating from the expansion of beneficial gut microbial activity. However, very few studies have established the metabolic consequences of interactions among specific fibers, microbiome composition, and function in either human or representative animal models. In a study design reflective of realistic population dietary variation, fecal metagenomic and metabolomic profiles were analyzed from healthy dogs fed 12 test foods containing different fiber sources and quantities (5–13% as-fed basis). Taxa and functions were identified whose abundances were associated either with overall fiber intake or with specific fiber compositions. Fourteen microbial species were significantly enriched in response to ≥1 specific fiber source; enrichment of fiber-derived metabolites was more pronounced in response to these fiber sources. Positively associated fecal metabolites, including short-chain fatty acids, acylglycerols, fiber bound sugars, and polyphenols, co-occurred with microbes enriched in specific food groups. Critically, the specific metabolite pools responsive to differential fiber intake were dependent on differences both in individual microbial community membership and in overall ecological configuration. This helps to explain, for the first time, differences in microbiome-diet associations observed in companion animal epidemiology. Thus, our study corroborates findings in human cohorts and reinforces the role of personalized microbiomes even in seemingly phenotypically homogeneous subjects.IMPORTANCEConsumption of dietary fiber changes the composition of the gut microbiome and, to a larger extent, the associated metabolites. Production of health-relevant metabolites such as short-chain fatty acids from fiber depends both on the consumption of a specific fiber and on the enrichment of beneficial metabolite-producing species in response to it. Even in a seemingly homogeneous population, the benefit received from fiber consumption is personalized and emphasizes specific fiber-microbe-host interactions. These observations are relevant for both population-wide and personalized nutrition applications. |
| format | Article |
| id | doaj-art-76469be4098d4d7ba5e8c3452318e3ee |
| institution | Kabale University |
| issn | 2379-5077 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSystems |
| spelling | doaj-art-76469be4098d4d7ba5e8c3452318e3ee2025-01-21T14:00:28ZengAmerican Society for MicrobiologymSystems2379-50772025-01-0110110.1128/msystems.00452-24Response of the gut microbiome and metabolome to dietary fiber in healthy dogsAmrisha Bhosle0Matthew I. Jackson1Aaron M. Walsh2Eric A. Franzosa3Dayakar V. Badri4Curtis Huttenhower5Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USAHill’s Pet Nutrition Inc., Topeka, Kansas, USADepartment of Biostatistics, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USAInfectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USAHill’s Pet Nutrition Inc., Topeka, Kansas, USAInfectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USAABSTRACT Dietary fiber confers multiple health benefits originating from the expansion of beneficial gut microbial activity. However, very few studies have established the metabolic consequences of interactions among specific fibers, microbiome composition, and function in either human or representative animal models. In a study design reflective of realistic population dietary variation, fecal metagenomic and metabolomic profiles were analyzed from healthy dogs fed 12 test foods containing different fiber sources and quantities (5–13% as-fed basis). Taxa and functions were identified whose abundances were associated either with overall fiber intake or with specific fiber compositions. Fourteen microbial species were significantly enriched in response to ≥1 specific fiber source; enrichment of fiber-derived metabolites was more pronounced in response to these fiber sources. Positively associated fecal metabolites, including short-chain fatty acids, acylglycerols, fiber bound sugars, and polyphenols, co-occurred with microbes enriched in specific food groups. Critically, the specific metabolite pools responsive to differential fiber intake were dependent on differences both in individual microbial community membership and in overall ecological configuration. This helps to explain, for the first time, differences in microbiome-diet associations observed in companion animal epidemiology. Thus, our study corroborates findings in human cohorts and reinforces the role of personalized microbiomes even in seemingly phenotypically homogeneous subjects.IMPORTANCEConsumption of dietary fiber changes the composition of the gut microbiome and, to a larger extent, the associated metabolites. Production of health-relevant metabolites such as short-chain fatty acids from fiber depends both on the consumption of a specific fiber and on the enrichment of beneficial metabolite-producing species in response to it. Even in a seemingly homogeneous population, the benefit received from fiber consumption is personalized and emphasizes specific fiber-microbe-host interactions. These observations are relevant for both population-wide and personalized nutrition applications.https://journals.asm.org/doi/10.1128/msystems.00452-24caninedietary fibermetabolomegut microbiomepersonalized nutrition |
| spellingShingle | Amrisha Bhosle Matthew I. Jackson Aaron M. Walsh Eric A. Franzosa Dayakar V. Badri Curtis Huttenhower Response of the gut microbiome and metabolome to dietary fiber in healthy dogs mSystems canine dietary fiber metabolome gut microbiome personalized nutrition |
| title | Response of the gut microbiome and metabolome to dietary fiber in healthy dogs |
| title_full | Response of the gut microbiome and metabolome to dietary fiber in healthy dogs |
| title_fullStr | Response of the gut microbiome and metabolome to dietary fiber in healthy dogs |
| title_full_unstemmed | Response of the gut microbiome and metabolome to dietary fiber in healthy dogs |
| title_short | Response of the gut microbiome and metabolome to dietary fiber in healthy dogs |
| title_sort | response of the gut microbiome and metabolome to dietary fiber in healthy dogs |
| topic | canine dietary fiber metabolome gut microbiome personalized nutrition |
| url | https://journals.asm.org/doi/10.1128/msystems.00452-24 |
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