Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe
Food safety is a major issue to public health and have attracted global attention. Fast, sensitive, and reliable detection methods for food hazardous substances is highly desirable. Aptamers which can bind to the target molecules with high affinity and specificity represent an attractive tool for th...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002270 |
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| author | Lei Wang Lili Yao Qihui Ma Yu Mao Hao Qu Lei Zheng |
| author_facet | Lei Wang Lili Yao Qihui Ma Yu Mao Hao Qu Lei Zheng |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Food safety is a major issue to public health and have attracted global attention. Fast, sensitive, and reliable detection methods for food hazardous substances is highly desirable. Aptamers which can bind to the target molecules with high affinity and specificity represent an attractive tool for the recognition of food hazardous substances, which play an important role in the development and application of new food safety detection technology. But current assays for characterizing small molecule-aptamer binding are limited by either the mass sensitivity or the size differentiation ability. Herein, we proposed a comprehensive method for assessing the dissociation equilibria of small molecule-aptamer, which is immobilized-free under ambient conditions. The design employs the Le Chatelier's principle and could be used to effectively measure small molecule-aptamer interactions. ATP binding aptamer and anti-aflatoxin B1 aptamer were used as the model system to determine their affinity, in which their dissociation equilibria measurements are in excellent close to their previous work. Due to the simplicity and sensitivity of this new method, we believe that it could be recommended as an effective tool for characterizing small molecule-aptamer interactions and promote the further application of small molecular aptamer in food safety. |
| format | Article |
| id | doaj-art-7641e3a3edd74a5ba63516b32de9e548 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-7641e3a3edd74a5ba63516b32de9e5482025-02-03T04:05:21ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112412571264Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probeLei Wang0Lili Yao1Qihui Ma2Yu Mao3Hao Qu4Lei Zheng5School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaCorresponding author at: Hefei University of Technology, Hefei 230009, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaCorresponding author at: Hefei University of Technology, Hefei 230009, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaFood safety is a major issue to public health and have attracted global attention. Fast, sensitive, and reliable detection methods for food hazardous substances is highly desirable. Aptamers which can bind to the target molecules with high affinity and specificity represent an attractive tool for the recognition of food hazardous substances, which play an important role in the development and application of new food safety detection technology. But current assays for characterizing small molecule-aptamer binding are limited by either the mass sensitivity or the size differentiation ability. Herein, we proposed a comprehensive method for assessing the dissociation equilibria of small molecule-aptamer, which is immobilized-free under ambient conditions. The design employs the Le Chatelier's principle and could be used to effectively measure small molecule-aptamer interactions. ATP binding aptamer and anti-aflatoxin B1 aptamer were used as the model system to determine their affinity, in which their dissociation equilibria measurements are in excellent close to their previous work. Due to the simplicity and sensitivity of this new method, we believe that it could be recommended as an effective tool for characterizing small molecule-aptamer interactions and promote the further application of small molecular aptamer in food safety.http://www.sciencedirect.com/science/article/pii/S2213453022002270AptamerSmall moleculeDissociation equilibriaAntisense displacement probeLe Chatelier's principle |
| spellingShingle | Lei Wang Lili Yao Qihui Ma Yu Mao Hao Qu Lei Zheng Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe Food Science and Human Wellness Aptamer Small molecule Dissociation equilibria Antisense displacement probe Le Chatelier's principle |
| title | Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe |
| title_full | Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe |
| title_fullStr | Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe |
| title_full_unstemmed | Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe |
| title_short | Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe |
| title_sort | investigation on small molecule aptamer dissociation equilibria based on antisense displacement probe |
| topic | Aptamer Small molecule Dissociation equilibria Antisense displacement probe Le Chatelier's principle |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002270 |
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