GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD
The aim of this study was to test the hypothesis that glutathione- S-transferase (GST) genotypes were associated with COPD. GSTP1, GSTM1 and GSTT1 genotypes were determined by DNA methods and GST activity spectrophotometrically in older male Caucasian Croats (non- -smokers, ex-smokers, and smokers)...
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Sciendo
2014-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | http://www.degruyter.com/view/j/acph.2014.64.issue-1/acph-2014-0003/acph-2014-0003.xml?format=INT |
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| author | Žuntar Irena Petlevski Roberta Dodig Slavica Popović-Grle Sanja |
| author_facet | Žuntar Irena Petlevski Roberta Dodig Slavica Popović-Grle Sanja |
| author_sort | Žuntar Irena |
| collection | DOAJ |
| description | The aim of this study was to test the hypothesis that glutathione- S-transferase (GST) genotypes were associated with COPD. GSTP1, GSTM1 and GSTT1 genotypes were determined by DNA methods and GST activity spectrophotometrically in older male Caucasian Croats (non- -smokers, ex-smokers, and smokers) with stable COPD (n = 30) and sex/age matched controls (n = 60). The distribution of GSTP1 genotypes and alleles in controls vs. COPD showed a statistical difference (p < 0.05). The odds ratio of CC/CT+TT (wild type GSTP1 exon 6 vs. joint heterozygous and mutant homozygous GSTP1 exon 6) was 10.000 and statistically different (p = 0.002). In this study, the GSTP1 mutant genotype of exon 5 (GG), as well as GSTP1 mutant and heterozygous genotypes of exon 6 (TT and CT), were suggested to be genetic contributors to COPD susceptibility. Null GSTM1, null GSTT1 and joint GSTM1/GSTT1 null genotypes were not disease associated. Serum GST was not associated with GST genotypes and COPD or smoking history in our study subjects. Conclusions drawn from the study should be further supported and clarified by studies with larger sample sizes. |
| format | Article |
| id | doaj-art-75d209b8f87540aaab3ef8a3c9138859 |
| institution | Kabale University |
| issn | 1330-0075 1846-9558 |
| language | English |
| publishDate | 2014-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-75d209b8f87540aaab3ef8a3c91388592025-02-02T02:02:00ZengSciendoActa Pharmaceutica1330-00751846-95582014-03-0164111712910.2478/acph-2014-0003acph-2014-0003GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPDŽuntar Irena0Petlevski Roberta1Dodig Slavica2Popović-Grle Sanja3University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb CroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb CroatiaChildren’s Hospital Srebrnjak Referral Centre for Clinical Paediatric Allergology of the Ministry of Health and Social Welfare, Zagreb, CroatiaUniversity Hospital for Pulmonary Diseases Jordanovac, Zagreb, CroatiaThe aim of this study was to test the hypothesis that glutathione- S-transferase (GST) genotypes were associated with COPD. GSTP1, GSTM1 and GSTT1 genotypes were determined by DNA methods and GST activity spectrophotometrically in older male Caucasian Croats (non- -smokers, ex-smokers, and smokers) with stable COPD (n = 30) and sex/age matched controls (n = 60). The distribution of GSTP1 genotypes and alleles in controls vs. COPD showed a statistical difference (p < 0.05). The odds ratio of CC/CT+TT (wild type GSTP1 exon 6 vs. joint heterozygous and mutant homozygous GSTP1 exon 6) was 10.000 and statistically different (p = 0.002). In this study, the GSTP1 mutant genotype of exon 5 (GG), as well as GSTP1 mutant and heterozygous genotypes of exon 6 (TT and CT), were suggested to be genetic contributors to COPD susceptibility. Null GSTM1, null GSTT1 and joint GSTM1/GSTT1 null genotypes were not disease associated. Serum GST was not associated with GST genotypes and COPD or smoking history in our study subjects. Conclusions drawn from the study should be further supported and clarified by studies with larger sample sizes.http://www.degruyter.com/view/j/acph.2014.64.issue-1/acph-2014-0003/acph-2014-0003.xml?format=INTglutathione S-transferases (GST)polymorphismCOPDalpha-1-antitrypsinlactate dehydrogenase |
| spellingShingle | Žuntar Irena Petlevski Roberta Dodig Slavica Popović-Grle Sanja GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD Acta Pharmaceutica glutathione S-transferases (GST) polymorphism COPD alpha-1-antitrypsin lactate dehydrogenase |
| title | GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD |
| title_full | GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD |
| title_fullStr | GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD |
| title_full_unstemmed | GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD |
| title_short | GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD |
| title_sort | gstp1 gstm1 and gstt1 genetic polymorphisms and total serum gst concentration in stable male copd |
| topic | glutathione S-transferases (GST) polymorphism COPD alpha-1-antitrypsin lactate dehydrogenase |
| url | http://www.degruyter.com/view/j/acph.2014.64.issue-1/acph-2014-0003/acph-2014-0003.xml?format=INT |
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