Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development
Circular RNA (circRNA) is a type of noncoding RNA that can interact with miRNAs to regulate gene expression. However, little is known concerning circRNA, which is crucial in the pathogenesis of lung cancer. To date, limited studies have explored the role of circ_0044516 in lung cancer progression. R...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/5510869 |
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| author | Yue-Wei Chen Qiu-Rong Du Yu-Juan He Wen-Shu Chen Wen-Yang Jiang Qi Gui Cheng-Cheng Xu Wei Wang Hong-Yun Cheng |
| author_facet | Yue-Wei Chen Qiu-Rong Du Yu-Juan He Wen-Shu Chen Wen-Yang Jiang Qi Gui Cheng-Cheng Xu Wei Wang Hong-Yun Cheng |
| author_sort | Yue-Wei Chen |
| collection | DOAJ |
| description | Circular RNA (circRNA) is a type of noncoding RNA that can interact with miRNAs to regulate gene expression. However, little is known concerning circRNA, which is crucial in the pathogenesis of lung cancer. To date, limited studies have explored the role of circ_0044516 in lung cancer progression. Recently, we observed that circ_0044516 expression levels were obviously elevated in lung cancer tissues and cells. A549 and SPCA1 cells were transfected with circ_0044516 siRNA. We observed that knockdown of circ_0044516 dramatically repressed cell proliferation, increased cell apoptosis, and repressed the cell cycle. Moreover, A549 and SPCA1 cell migration and invasion abilities were greatly repressed by circ_0044516 siRNA. Due to accumulating evidence demonstrating the vital role of cancer stem cells, their mechanism of involvement has drawn increasing attention in tumor progression and metastasis research. We also found that cancer stem cell properties were restrained by silencing circ_0044516 in A549 and SPC-A1 cells. Moreover, in vivo xenograft experiments showed that circ_0044516 downregulation reduced tumor growth. Mechanistically, in lung cancer and using bioinformatics, we demonstrated that circ_0044516 sponges miR-136 targeting MAT2A. Furthermore, rescue assays were carried out to identify that circ_0044516 modulates cell proliferation, invasion, and stemness by regulating miR-136 and MAT2A in lung cancer. In summary, our study revealed that the circ_0044516/miR-136/MAT2A axis is involved in lung cancer progression. Our findings may provide novel targets for diagnosis and therapeutic intervention in lung cancer patients. |
| format | Article |
| id | doaj-art-75bdff7c36d64f89bb9c2926a362eabf |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-75bdff7c36d64f89bb9c2926a362eabf2025-02-03T05:44:08ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/55108695510869Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer DevelopmentYue-Wei Chen0Qiu-Rong Du1Yu-Juan He2Wen-Shu Chen3Wen-Yang Jiang4Qi Gui5Cheng-Cheng Xu6Wei Wang7Hong-Yun Cheng8Department of Cardiothoracic Surgery, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Clinical Laboratory, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Clinical Laboratory, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Thoracic Surgery, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Oncology, Huai’an Second People’s Hospital and the Affiliated Huai’an Hospital of Xuzhou Medical University, Huai’an, ChinaDepartment of Oncology, Huai’an Second People’s Hospital and the Affiliated Huai’an Hospital of Xuzhou Medical University, Huai’an, ChinaCircular RNA (circRNA) is a type of noncoding RNA that can interact with miRNAs to regulate gene expression. However, little is known concerning circRNA, which is crucial in the pathogenesis of lung cancer. To date, limited studies have explored the role of circ_0044516 in lung cancer progression. Recently, we observed that circ_0044516 expression levels were obviously elevated in lung cancer tissues and cells. A549 and SPCA1 cells were transfected with circ_0044516 siRNA. We observed that knockdown of circ_0044516 dramatically repressed cell proliferation, increased cell apoptosis, and repressed the cell cycle. Moreover, A549 and SPCA1 cell migration and invasion abilities were greatly repressed by circ_0044516 siRNA. Due to accumulating evidence demonstrating the vital role of cancer stem cells, their mechanism of involvement has drawn increasing attention in tumor progression and metastasis research. We also found that cancer stem cell properties were restrained by silencing circ_0044516 in A549 and SPC-A1 cells. Moreover, in vivo xenograft experiments showed that circ_0044516 downregulation reduced tumor growth. Mechanistically, in lung cancer and using bioinformatics, we demonstrated that circ_0044516 sponges miR-136 targeting MAT2A. Furthermore, rescue assays were carried out to identify that circ_0044516 modulates cell proliferation, invasion, and stemness by regulating miR-136 and MAT2A in lung cancer. In summary, our study revealed that the circ_0044516/miR-136/MAT2A axis is involved in lung cancer progression. Our findings may provide novel targets for diagnosis and therapeutic intervention in lung cancer patients.http://dx.doi.org/10.1155/2021/5510869 |
| spellingShingle | Yue-Wei Chen Qiu-Rong Du Yu-Juan He Wen-Shu Chen Wen-Yang Jiang Qi Gui Cheng-Cheng Xu Wei Wang Hong-Yun Cheng Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development Journal of Immunology Research |
| title | Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development |
| title_full | Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development |
| title_fullStr | Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development |
| title_full_unstemmed | Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development |
| title_short | Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development |
| title_sort | circ 0044516 regulates mir 136 mat2a pathway to facilitate lung cancer development |
| url | http://dx.doi.org/10.1155/2021/5510869 |
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