Th1/Th2 Cells and Associated Cytokines in Acute Hepatitis E and Related Acute Liver Failure
Background and Aims. The involvement of cellular immunity in the development of hepatitis E virus (HEV) infection is rare. We aimed to study the roles of viral load and Th cell responses in acute hepatitis E (AHE) and HEV-related acute liver failure (HEV-ALF). Methods. We evaluated viral load and Th...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/6027361 |
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| author | Jian Wu Yurong Guo Xuan Lu Fen Huang Feifei Lv Daqiao Wei Anquan Shang Jinfeng Yang Qiaoling Pan Bin Jiang Jiong Yu Hongcui Cao Lanjuan Li |
| author_facet | Jian Wu Yurong Guo Xuan Lu Fen Huang Feifei Lv Daqiao Wei Anquan Shang Jinfeng Yang Qiaoling Pan Bin Jiang Jiong Yu Hongcui Cao Lanjuan Li |
| author_sort | Jian Wu |
| collection | DOAJ |
| description | Background and Aims. The involvement of cellular immunity in the development of hepatitis E virus (HEV) infection is rare. We aimed to study the roles of viral load and Th cell responses in acute hepatitis E (AHE) and HEV-related acute liver failure (HEV-ALF). Methods. We evaluated viral load and Th1/Th2 cytokine levels in 34 patients with HEV infection, including 17 each with AHE or HEV-ALF. Seventeen healthy controls (HCs) were also included who were negative for anti-HEV IgM and IgG. Results. There was no significant difference in viral load and HEV RNA in the AHE and HEV-ALF groups (both P>0.05). The Th lymphocyte levels (CD3+, CD4+) in the AHE and HEV-ALF groups were significantly higher than those in the HC group (both P<0.05), but there was no significant difference between the AHE and HEV-ALF groups (P>0.05). Both IFN-γ and IL-10 showed gradual upward trend from the HC group to the AHE (both P<0.01), but IFN-γ showed a sharp downward trend from the AHE group to the HEV-ALF group (P<0.01) and IL-4 showed gradual upward trend from the AHE group to the HEV-ALF group (P<0.01).There was no significant difference in Th1 and Th2 cytokines between the HEV RNA(+) group and HEV RNA(-) group (all P>0.05). Th2 bias was observed from the AHE (ratio=58.65) to HEV-ALF (ratio=1.20) groups. The level of IFN-γ was associated with the outcome of HEV-ALF patients. Conclusions. HEV viral load was not associated with aggravation of AHE, and the HEV-ALF patients showed significant Th2 bias, which may be involved in the aggravation of AHE. |
| format | Article |
| id | doaj-art-75bc584732ce4ee3ad67103a69c2eba5 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-75bc584732ce4ee3ad67103a69c2eba52025-02-03T01:27:56ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/60273616027361Th1/Th2 Cells and Associated Cytokines in Acute Hepatitis E and Related Acute Liver FailureJian Wu0Yurong Guo1Xuan Lu2Fen Huang3Feifei Lv4Daqiao Wei5Anquan Shang6Jinfeng Yang7Qiaoling Pan8Bin Jiang9Jiong Yu10Hongcui Cao11Lanjuan Li12State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou 310003, ChinaDepartment of Laboratory Medicine, Yancheng Hospital of Traditional Chinese Medicine, Affiliated to Nanjing University of Traditional Chinese Medicine, Yancheng 224000, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou 310003, ChinaMedical School, Kunming University of Science and Technology, 727 Jing Ming South Road, Kunming 650031, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaMedical School, Kunming University of Science and Technology, 727 Jing Ming South Road, Kunming 650031, ChinaDepartment of Clinical Laboratory, Shanghai Tongji Hospital, Tongji University School of Medicine, 389 Xincun Road, Shanghai 200065, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou 310003, ChinaDepartment of Laboratory Medicine, The Central Blood Station of Yancheng City, Yancheng, 224000 Jiangsu, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou 310003, ChinaBackground and Aims. The involvement of cellular immunity in the development of hepatitis E virus (HEV) infection is rare. We aimed to study the roles of viral load and Th cell responses in acute hepatitis E (AHE) and HEV-related acute liver failure (HEV-ALF). Methods. We evaluated viral load and Th1/Th2 cytokine levels in 34 patients with HEV infection, including 17 each with AHE or HEV-ALF. Seventeen healthy controls (HCs) were also included who were negative for anti-HEV IgM and IgG. Results. There was no significant difference in viral load and HEV RNA in the AHE and HEV-ALF groups (both P>0.05). The Th lymphocyte levels (CD3+, CD4+) in the AHE and HEV-ALF groups were significantly higher than those in the HC group (both P<0.05), but there was no significant difference between the AHE and HEV-ALF groups (P>0.05). Both IFN-γ and IL-10 showed gradual upward trend from the HC group to the AHE (both P<0.01), but IFN-γ showed a sharp downward trend from the AHE group to the HEV-ALF group (P<0.01) and IL-4 showed gradual upward trend from the AHE group to the HEV-ALF group (P<0.01).There was no significant difference in Th1 and Th2 cytokines between the HEV RNA(+) group and HEV RNA(-) group (all P>0.05). Th2 bias was observed from the AHE (ratio=58.65) to HEV-ALF (ratio=1.20) groups. The level of IFN-γ was associated with the outcome of HEV-ALF patients. Conclusions. HEV viral load was not associated with aggravation of AHE, and the HEV-ALF patients showed significant Th2 bias, which may be involved in the aggravation of AHE.http://dx.doi.org/10.1155/2020/6027361 |
| spellingShingle | Jian Wu Yurong Guo Xuan Lu Fen Huang Feifei Lv Daqiao Wei Anquan Shang Jinfeng Yang Qiaoling Pan Bin Jiang Jiong Yu Hongcui Cao Lanjuan Li Th1/Th2 Cells and Associated Cytokines in Acute Hepatitis E and Related Acute Liver Failure Journal of Immunology Research |
| title | Th1/Th2 Cells and Associated Cytokines in Acute Hepatitis E and Related Acute Liver Failure |
| title_full | Th1/Th2 Cells and Associated Cytokines in Acute Hepatitis E and Related Acute Liver Failure |
| title_fullStr | Th1/Th2 Cells and Associated Cytokines in Acute Hepatitis E and Related Acute Liver Failure |
| title_full_unstemmed | Th1/Th2 Cells and Associated Cytokines in Acute Hepatitis E and Related Acute Liver Failure |
| title_short | Th1/Th2 Cells and Associated Cytokines in Acute Hepatitis E and Related Acute Liver Failure |
| title_sort | th1 th2 cells and associated cytokines in acute hepatitis e and related acute liver failure |
| url | http://dx.doi.org/10.1155/2020/6027361 |
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