In vitro interleukin 4 and interferon-gamma production by mononuclear cells from atopic dermatitis patients
In order to elucidate further the possible role of specific cytokines in the pathogenesis of atopic dermatitis (AD) the in vitro production of interleukin 4 (IL-4) and interferon-gamma (IFN-γ) in patients with severe atopic dermatitis (n = 4) was compared with that in a group of non-atopic healthy c...
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| Format: | Article |
| Language: | English |
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Wiley
1993-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935193000584 |
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| _version_ | 1832560878056112128 |
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| author | Serge Well Friedhelm Diel |
| author_facet | Serge Well Friedhelm Diel |
| author_sort | Serge Well |
| collection | DOAJ |
| description | In order to elucidate further the possible role of specific cytokines in the pathogenesis of atopic dermatitis (AD) the in vitro production of interleukin 4 (IL-4) and interferon-gamma (IFN-γ) in patients with severe atopic dermatitis (n = 4) was compared with that in a group of non-atopic healthy controls. Overall IL-4 production by PHA- and PWM-driven PBMNCs was increased in controls during the first 48 h in culture. Addition of interleukin 2 (IL-2) into parallel cultures generated an insignificant (p > 0.05) increase in IL-4 production in AD patients compared with that from controls. IFN-γ production by PWM-stimulated PBMNCs was markedly decreased in AD patients compared with controls (p < 0.01). Addition of IL-2 (250 U/ml) to parallel cultures failed to restore IFN-γ production in AD patients. Finally, no IL-4 or IFN-γ activity could be detected in any of the sera. In conclusion, the data suggest a possible dysregulation of cytokine production in at least a subgroup of AD patients, with an impaired capacity to secrete IFN-γ, but a partially intact IL-4 generating capacity. |
| format | Article |
| id | doaj-art-75ae4fdf5c4c49d383db50a82dd6d4f5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1993-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-75ae4fdf5c4c49d383db50a82dd6d4f52025-02-03T01:26:29ZengWileyMediators of Inflammation0962-93511466-18611993-01-012641141510.1155/S0962935193000584In vitro interleukin 4 and interferon-gamma production by mononuclear cells from atopic dermatitis patientsSerge Well0Friedhelm Diel1Department of Nutrition, Fachhochschule Fulda, FB HE, Marquardstr. 35, Fulda 36039, GermanyDepartment of Nutrition, Fachhochschule Fulda, FB HE, Marquardstr. 35, Fulda 36039, GermanyIn order to elucidate further the possible role of specific cytokines in the pathogenesis of atopic dermatitis (AD) the in vitro production of interleukin 4 (IL-4) and interferon-gamma (IFN-γ) in patients with severe atopic dermatitis (n = 4) was compared with that in a group of non-atopic healthy controls. Overall IL-4 production by PHA- and PWM-driven PBMNCs was increased in controls during the first 48 h in culture. Addition of interleukin 2 (IL-2) into parallel cultures generated an insignificant (p > 0.05) increase in IL-4 production in AD patients compared with that from controls. IFN-γ production by PWM-stimulated PBMNCs was markedly decreased in AD patients compared with controls (p < 0.01). Addition of IL-2 (250 U/ml) to parallel cultures failed to restore IFN-γ production in AD patients. Finally, no IL-4 or IFN-γ activity could be detected in any of the sera. In conclusion, the data suggest a possible dysregulation of cytokine production in at least a subgroup of AD patients, with an impaired capacity to secrete IFN-γ, but a partially intact IL-4 generating capacity.http://dx.doi.org/10.1155/S0962935193000584 |
| spellingShingle | Serge Well Friedhelm Diel In vitro interleukin 4 and interferon-gamma production by mononuclear cells from atopic dermatitis patients Mediators of Inflammation |
| title | In vitro interleukin 4 and interferon-gamma production by mononuclear cells from atopic dermatitis patients |
| title_full | In vitro interleukin 4 and interferon-gamma production by mononuclear cells from atopic dermatitis patients |
| title_fullStr | In vitro interleukin 4 and interferon-gamma production by mononuclear cells from atopic dermatitis patients |
| title_full_unstemmed | In vitro interleukin 4 and interferon-gamma production by mononuclear cells from atopic dermatitis patients |
| title_short | In vitro interleukin 4 and interferon-gamma production by mononuclear cells from atopic dermatitis patients |
| title_sort | in vitro interleukin 4 and interferon gamma production by mononuclear cells from atopic dermatitis patients |
| url | http://dx.doi.org/10.1155/S0962935193000584 |
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