Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process
Number of sclerotic glomeruli increases during the aging process. Consequently, majority of remained nonsclerosed glomeruli become hypertrophic and some of them sclerotic, too. The aim of this study was to quantify the size and connective tissue content of nonsclerosed glomeruli and to evaluate the...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/845046 |
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| author | Vesna R. Stojanović Ivan D. Jovanović Sladjana Z. Ugrenović Ljiljana P. Vasović Vladimir S. Živković Miodrag V. Jocić Braca K. Kundalić Miljana N. Pavlović |
| author_facet | Vesna R. Stojanović Ivan D. Jovanović Sladjana Z. Ugrenović Ljiljana P. Vasović Vladimir S. Živković Miodrag V. Jocić Braca K. Kundalić Miljana N. Pavlović |
| author_sort | Vesna R. Stojanović |
| collection | DOAJ |
| description | Number of sclerotic glomeruli increases during the aging process. Consequently, majority of remained nonsclerosed glomeruli become hypertrophic and some of them sclerotic, too. The aim of this study was to quantify the size and connective tissue content of nonsclerosed glomeruli and to evaluate the percentage of hypertrophic ones in examined human cases during the aging. Material was right kidney's tissue of 30 cadavers obtained during routine autopsies. Cadavers were without previously diagnosed kidney disease, diabetes, hypertension, or any other systemic disease. Tissue specimens were routinely prepared for histological and morphometric analysis. Images of the histological slices were analyzed and captured under 400x magnification with digital camera. Further they were morphometrically and statistically analyzed with ImageJ and NCSS-PASS software. Multiple and linear regression of obtained morphometric parameters showed significant increase of glomerular connective tissue area and percentage. Cluster analysis showed the presence of two types of glomeruli. Second type was characterized with significantly larger size, connective tissue content, and significantly lower cellularity, in relation to the first type. Such glomeruli might be considered as hypertrophic. First type of glomeruli was predominant in younger cases, while second type of glomeruli was predominant in cases older than 55 years. |
| format | Article |
| id | doaj-art-75ad3f326336490f9cc811595bee9119 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-75ad3f326336490f9cc811595bee91192025-02-03T07:26:08ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/845046845046Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging ProcessVesna R. Stojanović0Ivan D. Jovanović1Sladjana Z. Ugrenović2Ljiljana P. Vasović3Vladimir S. Živković4Miodrag V. Jocić5Braca K. Kundalić6Miljana N. Pavlović7Department of Anatomy, University of Niš, Faculty of Medicine, Dr Zoran Djindjic Boulevard 81, 18000 Niš, SerbiaDepartment of Anatomy, University of Niš, Faculty of Medicine, Dr Zoran Djindjic Boulevard 81, 18000 Niš, SerbiaDepartment of Anatomy, University of Niš, Faculty of Medicine, Dr Zoran Djindjic Boulevard 81, 18000 Niš, SerbiaDepartment of Anatomy, University of Niš, Faculty of Medicine, Dr Zoran Djindjic Boulevard 81, 18000 Niš, SerbiaDepartment of Anatomy, University of Niš, Faculty of Medicine, Dr Zoran Djindjic Boulevard 81, 18000 Niš, SerbiaInstitute of Transfusiology, Military Medical Academy, Crnotravska 17, 11000 Belgrade, SerbiaDepartment of Anatomy, University of Niš, Faculty of Medicine, Dr Zoran Djindjic Boulevard 81, 18000 Niš, SerbiaDepartment of Anatomy, University of Niš, Faculty of Medicine, Dr Zoran Djindjic Boulevard 81, 18000 Niš, SerbiaNumber of sclerotic glomeruli increases during the aging process. Consequently, majority of remained nonsclerosed glomeruli become hypertrophic and some of them sclerotic, too. The aim of this study was to quantify the size and connective tissue content of nonsclerosed glomeruli and to evaluate the percentage of hypertrophic ones in examined human cases during the aging. Material was right kidney's tissue of 30 cadavers obtained during routine autopsies. Cadavers were without previously diagnosed kidney disease, diabetes, hypertension, or any other systemic disease. Tissue specimens were routinely prepared for histological and morphometric analysis. Images of the histological slices were analyzed and captured under 400x magnification with digital camera. Further they were morphometrically and statistically analyzed with ImageJ and NCSS-PASS software. Multiple and linear regression of obtained morphometric parameters showed significant increase of glomerular connective tissue area and percentage. Cluster analysis showed the presence of two types of glomeruli. Second type was characterized with significantly larger size, connective tissue content, and significantly lower cellularity, in relation to the first type. Such glomeruli might be considered as hypertrophic. First type of glomeruli was predominant in younger cases, while second type of glomeruli was predominant in cases older than 55 years.http://dx.doi.org/10.1100/2012/845046 |
| spellingShingle | Vesna R. Stojanović Ivan D. Jovanović Sladjana Z. Ugrenović Ljiljana P. Vasović Vladimir S. Živković Miodrag V. Jocić Braca K. Kundalić Miljana N. Pavlović Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process The Scientific World Journal |
| title | Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process |
| title_full | Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process |
| title_fullStr | Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process |
| title_full_unstemmed | Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process |
| title_short | Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process |
| title_sort | morphometric analysis of nonsclerosed glomeruli size and connective tissue content during the aging process |
| url | http://dx.doi.org/10.1100/2012/845046 |
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