Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice
ABSTRACT: Nuclear factor erythroid-derived 2-like 2 (Nrf2) is the master regulator of antioxidant defenses. High-intensity interval training (HIIT) has been proposed as a time-efficient training program and has become a substantial component of modern training program. In the present study, we evalu...
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Tsinghua University Press
2022-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000738 |
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| author | Yangwenjie Wang Yang Xiang Ruiqi Wang Xiangning Li Jianxiong Wang Siwang Yu Ying Zhang |
| author_facet | Yangwenjie Wang Yang Xiang Ruiqi Wang Xiangning Li Jianxiong Wang Siwang Yu Ying Zhang |
| author_sort | Yangwenjie Wang |
| collection | DOAJ |
| description | ABSTRACT: Nuclear factor erythroid-derived 2-like 2 (Nrf2) is the master regulator of antioxidant defenses. High-intensity interval training (HIIT) has been proposed as a time-efficient training program and has become a substantial component of modern training program. In the present study, we evaluated the effects of sulforaphane (SFN), a dietary isothiocyanate derived from cruciferous vegetables and a potent Nrf2 activator, on Nrf2-mediated antioxidant defense responses of skeletal muscle induced by exhaustive exercise in HIIT mice. Male C57BL/6J mice were randomly allocated into control group, HIIT group, and HIIT pretreated with SFN (HIIT+SFN) group. On the third day after completion of a 6-weeks HIIT protocol, an exhaustive treadmill test was conducted in all mice. Mice were intraperitoneally injected with SFN (HIIT+SFN group) or PBS (HIIT and control mice) 4 times in 3 days prior to the exhaustive treadmill test. The results indicated that the 6-weeks HIIT protocol did not increase the antioxidative capacity of skeletal muscle during exhaustive exercise. Importantly, SFN treatment improved antioxidative capacity of skeletal muscle in response to the acute exhaustive exercise by increasing mRNA and nucleoprotein expression of Nrf2 and these genes involved in antioxidant generation and decreasing blood creatine kinase (CK) and 4-hydroxy-2-nonenal (4-HNE)-modified protein levels in the HIIT mice. |
| format | Article |
| id | doaj-art-7589b0d503c34151bf1634039ba757c3 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-7589b0d503c34151bf1634039ba757c32025-02-03T02:27:47ZengTsinghua University PressFood Science and Human Wellness2213-45302022-09-0111513551361Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT miceYangwenjie Wang0Yang Xiang1Ruiqi Wang2Xiangning Li3Jianxiong Wang4Siwang Yu5Ying Zhang6School of Sport Science, Beijing Sport University, Beijing 100084, ChinaCollege of Physical Education, Yan’an University, Shaanxi 716000, ChinaSchool of Sport Science, Beijing Sport University, Beijing 100084, ChinaSchool of Sport Science, Beijing Sport University, Beijing 100084, ChinaFaculty of Health, Engineering, and Sciences, University of Southern Queensland, Toowoomba 4350, AustraliaState Key Laboratory of Natural and Biomimetic Drugs, Department of Molecular and Cellular Pharmacology, Peking University School of Pharmaceutical Sciences, Beijing 100191, ChinaSchool of Sport Science, Beijing Sport University, Beijing 100084, China; Corresponding author.ABSTRACT: Nuclear factor erythroid-derived 2-like 2 (Nrf2) is the master regulator of antioxidant defenses. High-intensity interval training (HIIT) has been proposed as a time-efficient training program and has become a substantial component of modern training program. In the present study, we evaluated the effects of sulforaphane (SFN), a dietary isothiocyanate derived from cruciferous vegetables and a potent Nrf2 activator, on Nrf2-mediated antioxidant defense responses of skeletal muscle induced by exhaustive exercise in HIIT mice. Male C57BL/6J mice were randomly allocated into control group, HIIT group, and HIIT pretreated with SFN (HIIT+SFN) group. On the third day after completion of a 6-weeks HIIT protocol, an exhaustive treadmill test was conducted in all mice. Mice were intraperitoneally injected with SFN (HIIT+SFN group) or PBS (HIIT and control mice) 4 times in 3 days prior to the exhaustive treadmill test. The results indicated that the 6-weeks HIIT protocol did not increase the antioxidative capacity of skeletal muscle during exhaustive exercise. Importantly, SFN treatment improved antioxidative capacity of skeletal muscle in response to the acute exhaustive exercise by increasing mRNA and nucleoprotein expression of Nrf2 and these genes involved in antioxidant generation and decreasing blood creatine kinase (CK) and 4-hydroxy-2-nonenal (4-HNE)-modified protein levels in the HIIT mice.http://www.sciencedirect.com/science/article/pii/S2213453022000738SulforaphaneNrf2 (nuclear factor erythroid-derived 2-like 2)Skeletal muscleAntioxidantHIIT (high-intensity interval training)Mice |
| spellingShingle | Yangwenjie Wang Yang Xiang Ruiqi Wang Xiangning Li Jianxiong Wang Siwang Yu Ying Zhang Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice Food Science and Human Wellness Sulforaphane Nrf2 (nuclear factor erythroid-derived 2-like 2) Skeletal muscle Antioxidant HIIT (high-intensity interval training) Mice |
| title | Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice |
| title_full | Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice |
| title_fullStr | Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice |
| title_full_unstemmed | Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice |
| title_short | Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice |
| title_sort | sulforaphane enhances nrf2 mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in hiit mice |
| topic | Sulforaphane Nrf2 (nuclear factor erythroid-derived 2-like 2) Skeletal muscle Antioxidant HIIT (high-intensity interval training) Mice |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000738 |
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