Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture
Low intensity laser irradiation (LILI) was found to promote the regeneration of skeletal muscle in vivo but the cellular mechanisms are not fully understood. Myoblasts, normally quiescent and inactivated in adult skeletal muscle, are a type of myogenic progenitor cells and considered as the major ca...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/2014/205839 |
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| author | Cui-Ping Zhang Shao-Dan Li Yan Chen Yan-Ming Jiang Peng Chen Chang-Zhen Wang Xiao-Bing Fu Hong-Xiang Kang Ben-Jian Shen Jie Liang |
| author_facet | Cui-Ping Zhang Shao-Dan Li Yan Chen Yan-Ming Jiang Peng Chen Chang-Zhen Wang Xiao-Bing Fu Hong-Xiang Kang Ben-Jian Shen Jie Liang |
| author_sort | Cui-Ping Zhang |
| collection | DOAJ |
| description | Low intensity laser irradiation (LILI) was found to promote the regeneration of skeletal muscle in vivo but the cellular mechanisms are not fully understood. Myoblasts, normally quiescent and inactivated in adult skeletal muscle, are a type of myogenic progenitor cells and considered as the major candidates responsible for muscle regeneration. The aim of the present study was to study the effect of LILI on the growth potential and cell-cycle progression of the cultured myoblasts. Primary myoblasts isolated from rat hind legs were cultured in nutrient-deficient medium for 36 hours and then irradiated by helium-neon laser at a certain energy density. Immunohistochemical and flow cytometric analysis revealed that laser irradiation could increase the expression of cellular proliferation marker and the amount of cell subpopulations in the proliferative phase as compared with the nonirradiated control group. Meanwhile, the expressions of cell-cycle regulatory proteins in the laser-treated myoblasts were markedly upregulated as compared to the unirradiated cells, indicating that LILI could promote the reentry of quiescent myoblasts into the cell division cycle. These results suggest that LILI at certain fluences could promote their proliferation, thus contributing to the skeletal muscle regeneration following trauma and myopathic diseases. |
| format | Article |
| id | doaj-art-7558dc16e5d445b08ffdc7c97d1b8f58 |
| institution | Kabale University |
| issn | 1110-662X 1687-529X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-7558dc16e5d445b08ffdc7c97d1b8f582025-02-03T01:12:34ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2014-01-01201410.1155/2014/205839205839Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in CultureCui-Ping Zhang0Shao-Dan Li1Yan Chen2Yan-Ming Jiang3Peng Chen4Chang-Zhen Wang5Xiao-Bing Fu6Hong-Xiang Kang7Ben-Jian Shen8Jie Liang9Department of Wound Healing and Cell Biology, Burns Institute, The First Affiliated Hospital, General Hospital of Chinese People’s Liberation Army, Beijing 100048, ChinaInstitute of Traditional Chinese Medicine, General Hospital of Chinese People’s Liberation Army, Beijing 100853, ChinaDepartment of Pharmacy, General Hospital of Beijing Military Region, Beijing 100700, ChinaDepartment of Ophthalmology, Second Artillery Gerenal Hospital of Chinese People’s Liberation Army, Beijing 100088, ChinaDepartment of Electromagnetic and Laser Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, ChinaDepartment of Electromagnetic and Laser Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, ChinaDepartment of Wound Healing and Cell Biology, Burns Institute, The First Affiliated Hospital, General Hospital of Chinese People’s Liberation Army, Beijing 100048, ChinaDepartment of Electromagnetic and Laser Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, ChinaDepartment of Electromagnetic and Laser Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, ChinaDepartment of Electromagnetic and Laser Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, ChinaLow intensity laser irradiation (LILI) was found to promote the regeneration of skeletal muscle in vivo but the cellular mechanisms are not fully understood. Myoblasts, normally quiescent and inactivated in adult skeletal muscle, are a type of myogenic progenitor cells and considered as the major candidates responsible for muscle regeneration. The aim of the present study was to study the effect of LILI on the growth potential and cell-cycle progression of the cultured myoblasts. Primary myoblasts isolated from rat hind legs were cultured in nutrient-deficient medium for 36 hours and then irradiated by helium-neon laser at a certain energy density. Immunohistochemical and flow cytometric analysis revealed that laser irradiation could increase the expression of cellular proliferation marker and the amount of cell subpopulations in the proliferative phase as compared with the nonirradiated control group. Meanwhile, the expressions of cell-cycle regulatory proteins in the laser-treated myoblasts were markedly upregulated as compared to the unirradiated cells, indicating that LILI could promote the reentry of quiescent myoblasts into the cell division cycle. These results suggest that LILI at certain fluences could promote their proliferation, thus contributing to the skeletal muscle regeneration following trauma and myopathic diseases.http://dx.doi.org/10.1155/2014/205839 |
| spellingShingle | Cui-Ping Zhang Shao-Dan Li Yan Chen Yan-Ming Jiang Peng Chen Chang-Zhen Wang Xiao-Bing Fu Hong-Xiang Kang Ben-Jian Shen Jie Liang Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture International Journal of Photoenergy |
| title | Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture |
| title_full | Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture |
| title_fullStr | Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture |
| title_full_unstemmed | Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture |
| title_short | Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture |
| title_sort | stimulative effects of low intensity he ne laser irradiation on the proliferative potential and cell cycle progression of myoblasts in culture |
| url | http://dx.doi.org/10.1155/2014/205839 |
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