The Validation of the sickle scan technique used to diagnose sickle cell disease
Introduction Sickle cell disease is a multifactorial haemoglobinopathy that causes various abnormalities in affected individuals. Several diagnostic methods exist, but most require sophisticated techniques that are not available in Congolese diagnostic centres involved in this activity. Conside...
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Orapuh, Inc.
2025-01-01
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| Series: | Orapuh Journal |
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| Online Access: | https://orapuh.org/ojs/ojs-3.1.2-4/index.php/orapj/article/view/318 |
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| author | Magain Mihatano Barihuta Guelord Inkalaba Jocelyn Kakumba Mankulu Olivier Nzingula Maguy Luvandu Mbenza Nicole M. Misengabu M Ndeme Michel Diyi Mbembe Bitengeli Tresor Bayebila Menanzambi Nsimba Miezi José Lami Nzunzu Fridolin Kodondi Kule-koto |
| author_facet | Magain Mihatano Barihuta Guelord Inkalaba Jocelyn Kakumba Mankulu Olivier Nzingula Maguy Luvandu Mbenza Nicole M. Misengabu M Ndeme Michel Diyi Mbembe Bitengeli Tresor Bayebila Menanzambi Nsimba Miezi José Lami Nzunzu Fridolin Kodondi Kule-koto |
| author_sort | Magain Mihatano Barihuta |
| collection | DOAJ |
| description |
Introduction
Sickle cell disease is a multifactorial haemoglobinopathy that causes various abnormalities in affected individuals. Several diagnostic methods exist, but most require sophisticated techniques that are not available in Congolese diagnostic centres involved in this activity. Considering the number of affected individuals in Africa, particularly in the Democratic Republic of Congo (The Democratic Republic of the Congo) (50 million people affected), and the precarious financial situation of our countries, it is essential to develop low-cost techniques to facilitate access to screening for our populations.
Purpose
This study aimed to validate the rapid "Sickle SCAN" method, manufactured by ZENTECH, as a tool for rapid diagnostic testing.
Methods
We assessed a single validation criterion: selectivity. This was done using samples with known haemoglobin status. The results of the Sickle SCAN test obtained from 290 subjects were compared with those obtained through isoelectric focusing, the reference method, using the Chi-square test. The duration for trait appearance was also evaluated.
Results
The Sickle SCAN test demonstrated 100% selectivity. Statistical analysis using the Chi-square test confirmed that the results from isoelectric focusing were identical to those obtained using the Sickle SCAN method. This test can also be applied to samples collected on blotting paper. The interpretation time should not exceed 2 minutes, contrary to the manufacturer's claims.
Conclusion
The Sickle SCAN rapid screening test, manufactured by ZENTECH, produced satisfactory results and is suitable for use by the Congolese population to screen for sickle cell disease. This test has the advantages of being accessible, available, and easy to use.
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| format | Article |
| id | doaj-art-754b5e6ac4e64e7b8bdb226d46c3da0f |
| institution | Kabale University |
| issn | 2644-3740 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Orapuh, Inc. |
| record_format | Article |
| series | Orapuh Journal |
| spelling | doaj-art-754b5e6ac4e64e7b8bdb226d46c3da0f2025-01-30T06:12:04ZengOrapuh, Inc.Orapuh Journal2644-37402025-01-016210.4314/orapj.v6i1.11The Validation of the sickle scan technique used to diagnose sickle cell diseaseMagain Mihatano Barihuta0Guelord Inkalaba1Jocelyn Kakumba Mankulu 2Olivier Nzingula3Maguy Luvandu Mbenza4Nicole M. Misengabu5M Ndeme6Michel Diyi7Mbembe Bitengeli8Tresor Bayebila Menanzambi9Nsimba Miezi10José Lami Nzunzu11Fridolin Kodondi Kule-koto12University of Kinshasa, Kinshasa XI, Democratic Republic of the CongoUniversity of Kinshasa, Kinshasa XI, Democratic Republic of the CongoUniversity of Kinshasa, Kinshasa XI, Democratic Republic of the CongoUniversity of Kinshasa, Kinshasa XI, Democratic Republic of the CongoUniversity of Kinshasa, Kinshasa XI, Democratic Republic of the CongoInstitute of Research in Health Sciences, Kinshasa, Democratic Republic of the CongoInstitute of Research in Health Sciences, Kinshasa, Democratic Republic of the CongoInstitute of Research in Health Sciences, Kinshasa, Democratic Republic of the CongoInstitute of Research in Health Sciences, Kinshasa, Democratic Republic of the CongoUniversity of Kinshasa, Kinshasa XI, Democratic Republic of the CongoUniversity of Kinshasa, Kinshasa XI, Democratic Republic of the CongoUniversity of Kinshasa, Kinshasa XI, Democratic Republic of the CongoUniversity of Kinshasa, Kinshasa XI, Democratic Republic of the Congo Introduction Sickle cell disease is a multifactorial haemoglobinopathy that causes various abnormalities in affected individuals. Several diagnostic methods exist, but most require sophisticated techniques that are not available in Congolese diagnostic centres involved in this activity. Considering the number of affected individuals in Africa, particularly in the Democratic Republic of Congo (The Democratic Republic of the Congo) (50 million people affected), and the precarious financial situation of our countries, it is essential to develop low-cost techniques to facilitate access to screening for our populations. Purpose This study aimed to validate the rapid "Sickle SCAN" method, manufactured by ZENTECH, as a tool for rapid diagnostic testing. Methods We assessed a single validation criterion: selectivity. This was done using samples with known haemoglobin status. The results of the Sickle SCAN test obtained from 290 subjects were compared with those obtained through isoelectric focusing, the reference method, using the Chi-square test. The duration for trait appearance was also evaluated. Results The Sickle SCAN test demonstrated 100% selectivity. Statistical analysis using the Chi-square test confirmed that the results from isoelectric focusing were identical to those obtained using the Sickle SCAN method. This test can also be applied to samples collected on blotting paper. The interpretation time should not exceed 2 minutes, contrary to the manufacturer's claims. Conclusion The Sickle SCAN rapid screening test, manufactured by ZENTECH, produced satisfactory results and is suitable for use by the Congolese population to screen for sickle cell disease. This test has the advantages of being accessible, available, and easy to use. https://orapuh.org/ojs/ojs-3.1.2-4/index.php/orapj/article/view/318Drepanocytosissickle cell diseaseSickle SCANisoelectric focusing |
| spellingShingle | Magain Mihatano Barihuta Guelord Inkalaba Jocelyn Kakumba Mankulu Olivier Nzingula Maguy Luvandu Mbenza Nicole M. Misengabu M Ndeme Michel Diyi Mbembe Bitengeli Tresor Bayebila Menanzambi Nsimba Miezi José Lami Nzunzu Fridolin Kodondi Kule-koto The Validation of the sickle scan technique used to diagnose sickle cell disease Orapuh Journal Drepanocytosis sickle cell disease Sickle SCAN isoelectric focusing |
| title | The Validation of the sickle scan technique used to diagnose sickle cell disease |
| title_full | The Validation of the sickle scan technique used to diagnose sickle cell disease |
| title_fullStr | The Validation of the sickle scan technique used to diagnose sickle cell disease |
| title_full_unstemmed | The Validation of the sickle scan technique used to diagnose sickle cell disease |
| title_short | The Validation of the sickle scan technique used to diagnose sickle cell disease |
| title_sort | validation of the sickle scan technique used to diagnose sickle cell disease |
| topic | Drepanocytosis sickle cell disease Sickle SCAN isoelectric focusing |
| url | https://orapuh.org/ojs/ojs-3.1.2-4/index.php/orapj/article/view/318 |
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