Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial Isolates
Vernonia amygdalina is a plant known to contain substances with reported antimicrobial properties against various microorganisms. This study therefore, aimed at assessing the antibacterial properties of Vernonia amygdalina extract against some clinical bacterial isolates. The bacterial isolates wer...
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Umaru Musa Yar'adua University, Katsina, Nigeria
2023-12-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/400 |
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| author | Auwal Magaji Zinat Mahmud Aminu Mustafa |
| author_facet | Auwal Magaji Zinat Mahmud Aminu Mustafa |
| author_sort | Auwal Magaji |
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Vernonia amygdalina is a plant known to contain substances with reported antimicrobial properties against various microorganisms. This study therefore, aimed at assessing the antibacterial properties of Vernonia amygdalina extract against some clinical bacterial isolates. The bacterial isolates were obtained from General hospital Azare, and the Vernonia amygdalina leaves were purchased at Azare central market. Various compounds, including saponins, glycosides, tannins, flavonoids, steroids, phenolic compounds, and alkaloids, were identified in the plant extract via phytochemical screening. Different concentrations of the V. amygdalina extract (25mg/mL, 50mg/mL, and 100mg/mL) were prepared and screened for antibacterial using disc diffusion method, revealing zones of inhibition of 7.00 mm, 9.00 mm and 4.00 mm against Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae respectively, at 100mg/ml of the extract. The MIC of the extract against Escherichia coli and Staphylococcus aureus was 25 mg/mL, whereas 50 mg/mL of the extract was found to be the minimum concentration active against Klebsiella pneumoniae. The study found that the extract exhibited modest antibacterial activity, with different minimum inhibitory concentrations for the bacterial strains.
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| format | Article |
| id | doaj-art-75479bcd22e14de0bc5bcbfb3a32c8bc |
| institution | OA Journals |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-75479bcd22e14de0bc5bcbfb3a32c8bc2025-08-20T02:06:43ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222023-12-018210.47430/ujmr.2382.020400Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial IsolatesAuwal Magaji0Zinat Mahmud1Aminu Mustafa2Department of microbiology, Bauchi State University Gadau, Bauchi State NigeriaDepartment of microbiology, Bauchi State University Gadau, Bauchi State NigeriaDepartment of microbiology, Bauchi State University Gadau, Bauchi State Nigeria Vernonia amygdalina is a plant known to contain substances with reported antimicrobial properties against various microorganisms. This study therefore, aimed at assessing the antibacterial properties of Vernonia amygdalina extract against some clinical bacterial isolates. The bacterial isolates were obtained from General hospital Azare, and the Vernonia amygdalina leaves were purchased at Azare central market. Various compounds, including saponins, glycosides, tannins, flavonoids, steroids, phenolic compounds, and alkaloids, were identified in the plant extract via phytochemical screening. Different concentrations of the V. amygdalina extract (25mg/mL, 50mg/mL, and 100mg/mL) were prepared and screened for antibacterial using disc diffusion method, revealing zones of inhibition of 7.00 mm, 9.00 mm and 4.00 mm against Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae respectively, at 100mg/ml of the extract. The MIC of the extract against Escherichia coli and Staphylococcus aureus was 25 mg/mL, whereas 50 mg/mL of the extract was found to be the minimum concentration active against Klebsiella pneumoniae. The study found that the extract exhibited modest antibacterial activity, with different minimum inhibitory concentrations for the bacterial strains. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/400PhytochemicalantibacterialVernonia amygdalina. |
| spellingShingle | Auwal Magaji Zinat Mahmud Aminu Mustafa Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial Isolates UMYU Journal of Microbiology Research Phytochemical antibacterial Vernonia amygdalina. |
| title | Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial Isolates |
| title_full | Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial Isolates |
| title_fullStr | Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial Isolates |
| title_full_unstemmed | Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial Isolates |
| title_short | Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial Isolates |
| title_sort | phytochemical analysis and assessment of antibacterial efficacy of vernonia amygdalina bitter leaf against some selected clinical bacterial isolates |
| topic | Phytochemical antibacterial Vernonia amygdalina. |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/400 |
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