Optimization of Butanol Production from Mixed Sugars and Sweet Sorghum Bagasse Hydrolysate Using <i>Clostridium beijerinckii</i> TISTR 1461

Optimization of Butanol Production from Mixed Sugars and Sweet Sorghum Bagasse Hydrolysate Using <i>Clostridium beijerinckii</i> TISTR 1461

This study investigated the capability of <i>Clostridium beijerinckii</i> TISTR 1461 to utilize mixed sugars (glucose and xylose) in synthetic media and sweet sorghum bagasse (SSB) hydrolysate for butanol production. Synthetic media containing 60 g/L of glucose and xylose at various rati...

Full description

Saved in:
Bibliographic Details
Main Authors: Chalida Daengbussadee, Lakkana Laopaiboon, Thanawat Thanapornsin, Pattana Laopaiboon
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Energies
Subjects:
biobutanol
<i>Clostridium beijerinckii</i>
glucose and xylose
mixed sugars
sweet sorghum bagasse
Online Access:https://www.mdpi.com/1996-1073/18/2/306
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study investigated the capability of <i>Clostridium beijerinckii</i> TISTR 1461 to utilize mixed sugars (glucose and xylose) in synthetic media and sweet sorghum bagasse (SSB) hydrolysate for butanol production. Synthetic media containing 60 g/L of glucose and xylose at various ratios were used for butanol production. <i>C. beijerinckii</i> TISTR 1461 preferentially utilized glucose over xylose for butanol production. The highest butanol concentration (<i>P<sub>B</sub></i>, 10.25–10.60 g/L), butanol yield (<i>Y<sub>B/S</sub></i>, 0.27–0.28 g/g), butanol productivity (<i>Q<sub>B</sub></i>, 0.22 g/L·h), and sugar consumption (<i>SC</i>, 61–63%) were achieved when the glucose content was at least 75% of the total sugars. When an SSB hydrolysate (produced via enzymatic hydrolysis) containing 60.83 g/L of total sugars (glucose:xylose ratio = 88:12, <i>w</i>/<i>w</i>) was used as a substrate for butanol production, the SSB hydrolysate supplemented with 1 g/L of yeast extract and buffers significantly yielded higher <i>P<sub>B</sub></i> (15.10 g/L), <i>Y<sub>B/S</sub></i> (0.31 g/g), <i>Q<sub>B</sub></i> (0.31 g/L·h), and <i>SC</i> (82%) values compared to the synthetic media. These results indicate that sweet sorghum bagasse hydrolysates containing glucose and xylose mixtures show promise as cost-effective substrates for sustainable butanol fermentation, demonstrating the potential of agricultural residues in biofuel production.
ISSN:1996-1073

Similar Items