Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells
The CRISPR/Cas9 system provides a powerful method for the genetic manipulation of the mammalian genome, allowing knockout of individual genes as well as the generation of genome-wide knockout cell libraries for genetic screening. However, the diploid status of most mammalian cells restricts the appl...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/2601746 |
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| _version_ | 1832555925916876800 |
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| author | Zixi Yin Lingyi Chen |
| author_facet | Zixi Yin Lingyi Chen |
| author_sort | Zixi Yin |
| collection | DOAJ |
| description | The CRISPR/Cas9 system provides a powerful method for the genetic manipulation of the mammalian genome, allowing knockout of individual genes as well as the generation of genome-wide knockout cell libraries for genetic screening. However, the diploid status of most mammalian cells restricts the application of CRISPR/Cas9 in genetic screening. Mammalian haploid embryonic stem cells (haESCs) have only one set of chromosomes per cell, avoiding the issue of heterozygous recessive mutations in diploid cells. Thus, the combination of haESCs and CRISPR/Cas9 facilitates the generation of genome-wide knockout cell libraries for genetic screening. Here, we review recent progress in CRISPR/Cas9 and haPSCs and discuss their applications in genetic screening. |
| format | Article |
| id | doaj-art-753870d005914949a8e635f8b9524f79 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-753870d005914949a8e635f8b9524f792025-02-03T05:46:51ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/26017462601746Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem CellsZixi Yin0Lingyi Chen1State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Collaborative Innovation Center for Biotherapy, Tianjin Key Laboratory of Protein Sciences, 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics and College of Life Sciences, Nankai University, Tianjin 300071, ChinaState Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Collaborative Innovation Center for Biotherapy, Tianjin Key Laboratory of Protein Sciences, 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics and College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe CRISPR/Cas9 system provides a powerful method for the genetic manipulation of the mammalian genome, allowing knockout of individual genes as well as the generation of genome-wide knockout cell libraries for genetic screening. However, the diploid status of most mammalian cells restricts the application of CRISPR/Cas9 in genetic screening. Mammalian haploid embryonic stem cells (haESCs) have only one set of chromosomes per cell, avoiding the issue of heterozygous recessive mutations in diploid cells. Thus, the combination of haESCs and CRISPR/Cas9 facilitates the generation of genome-wide knockout cell libraries for genetic screening. Here, we review recent progress in CRISPR/Cas9 and haPSCs and discuss their applications in genetic screening.http://dx.doi.org/10.1155/2017/2601746 |
| spellingShingle | Zixi Yin Lingyi Chen Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells Stem Cells International |
| title | Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells |
| title_full | Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells |
| title_fullStr | Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells |
| title_full_unstemmed | Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells |
| title_short | Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells |
| title_sort | simple meets single the application of crispr cas9 in haploid embryonic stem cells |
| url | http://dx.doi.org/10.1155/2017/2601746 |
| work_keys_str_mv | AT zixiyin simplemeetssingletheapplicationofcrisprcas9inhaploidembryonicstemcells AT lingyichen simplemeetssingletheapplicationofcrisprcas9inhaploidembryonicstemcells |