Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary Infections
ABSTRACT Objective In this study, we investigated the application value of bronchoalveolar lavage fluid (BALF) combined with targeted next‐generation sequencing (tNGS) in the pathogen detection‐based diagnosis of patients with lung infections. Method A retrospective analysis was conducted on patient...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | The Clinical Respiratory Journal |
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| Online Access: | https://doi.org/10.1111/crj.70046 |
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| author | Jiangbo Liu Bo Yang Yu Wu Guihong Yang Xiaojiu Zha Wei Jiang |
| author_facet | Jiangbo Liu Bo Yang Yu Wu Guihong Yang Xiaojiu Zha Wei Jiang |
| author_sort | Jiangbo Liu |
| collection | DOAJ |
| description | ABSTRACT Objective In this study, we investigated the application value of bronchoalveolar lavage fluid (BALF) combined with targeted next‐generation sequencing (tNGS) in the pathogen detection‐based diagnosis of patients with lung infections. Method A retrospective analysis was conducted on patients who underwent tracheoscopy and conventional microbiological tests (CMTs) on BALF, coupled with metagenomic next‐generation sequencing (mNGS) or tNGS. This investigation encompassed individuals with suspected lung infections at Tianjin First Central Hospital from March 2023 to July 2023. Diagnostic rates based on pathogens detected via tNGS were compared with CMTs within the tNGS group. Additionally, diagnostic rates obtained through tNGS were compared with mNGS between the two groups. Results The data of a total of 169 patients (78 in the tNGS group and 91 in the mNGS group) were collected, and 145 patients (67 in the tNGS group and 78 in the mNGS group) were finally diagnosed with lung infections. The comprehensive positive pathogen detection‐based diagnosis rate for tNGS was 86.6%, with a single‐pathogen lung infection diagnosis rate of 85.7% and a mixed‐pathogen pulmonary infection diagnosis rate of 88.0%. In contrast, the overall positive pathogen detection‐based diagnosis rate for CMTs was 38.8%, comprising a single‐pathogen pulmonary infection diagnosis rate of 28.6% and a mixed‐pathogen pulmonary infection diagnosis rate of 20.0%. The difference in positive diagnosis rate was deemed statistically significant (p < 0.05). In the mNGS group, the overall pathogen detection‐based diagnosis rate was 89.7%, with a single‐pathogen pulmonary infection diagnosis rate of 84.9%, and a 100% diagnosis rate for mixed‐pathogen pulmonary infections. There was no statistically significant difference in the positive diagnosis rate when compared with the tNGS group (p > 0.05). Conclusion In patients with pulmonary infections, the diagnosis rate based on BALF pathogen detection using tNGS exceeded that of CMTs, showing no statistically significant difference compared to mNGS. |
| format | Article |
| id | doaj-art-75101b0651734366ba06bc8937878fe2 |
| institution | Kabale University |
| issn | 1752-6981 1752-699X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Clinical Respiratory Journal |
| spelling | doaj-art-75101b0651734366ba06bc8937878fe22025-01-27T08:28:30ZengWileyThe Clinical Respiratory Journal1752-69811752-699X2025-01-01191n/an/a10.1111/crj.70046Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary InfectionsJiangbo Liu0Bo Yang1Yu Wu2Guihong Yang3Xiaojiu Zha4Wei Jiang5Department of Pulmonary and Critical Care Medicine Tianjin First Central Hospital Tianjin ChinaDepartment of Thoracic Surgery Tianjin First Central Hospital Tianjin ChinaDepartment of Pulmonary and Critical Care Medicine Tianjin First Central Hospital Tianjin ChinaDepartment of Pulmonary Diseases Dafang County Traditional Chinese Medicine Hospital Bijie Guizhou ChinaDepartment of Pulmonary and Critical Care Medicine Tianjin First Central Hospital Tianjin ChinaInfectious Diseases Department Tianjin First Central Hospital Tianjin ChinaABSTRACT Objective In this study, we investigated the application value of bronchoalveolar lavage fluid (BALF) combined with targeted next‐generation sequencing (tNGS) in the pathogen detection‐based diagnosis of patients with lung infections. Method A retrospective analysis was conducted on patients who underwent tracheoscopy and conventional microbiological tests (CMTs) on BALF, coupled with metagenomic next‐generation sequencing (mNGS) or tNGS. This investigation encompassed individuals with suspected lung infections at Tianjin First Central Hospital from March 2023 to July 2023. Diagnostic rates based on pathogens detected via tNGS were compared with CMTs within the tNGS group. Additionally, diagnostic rates obtained through tNGS were compared with mNGS between the two groups. Results The data of a total of 169 patients (78 in the tNGS group and 91 in the mNGS group) were collected, and 145 patients (67 in the tNGS group and 78 in the mNGS group) were finally diagnosed with lung infections. The comprehensive positive pathogen detection‐based diagnosis rate for tNGS was 86.6%, with a single‐pathogen lung infection diagnosis rate of 85.7% and a mixed‐pathogen pulmonary infection diagnosis rate of 88.0%. In contrast, the overall positive pathogen detection‐based diagnosis rate for CMTs was 38.8%, comprising a single‐pathogen pulmonary infection diagnosis rate of 28.6% and a mixed‐pathogen pulmonary infection diagnosis rate of 20.0%. The difference in positive diagnosis rate was deemed statistically significant (p < 0.05). In the mNGS group, the overall pathogen detection‐based diagnosis rate was 89.7%, with a single‐pathogen pulmonary infection diagnosis rate of 84.9%, and a 100% diagnosis rate for mixed‐pathogen pulmonary infections. There was no statistically significant difference in the positive diagnosis rate when compared with the tNGS group (p > 0.05). Conclusion In patients with pulmonary infections, the diagnosis rate based on BALF pathogen detection using tNGS exceeded that of CMTs, showing no statistically significant difference compared to mNGS.https://doi.org/10.1111/crj.70046bronchoalveolar lavage fluidconventional microbiological testsmetagenomic next‐generation sequencingpulmonary infectiontargeted next‐generation sequencing |
| spellingShingle | Jiangbo Liu Bo Yang Yu Wu Guihong Yang Xiaojiu Zha Wei Jiang Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary Infections The Clinical Respiratory Journal bronchoalveolar lavage fluid conventional microbiological tests metagenomic next‐generation sequencing pulmonary infection targeted next‐generation sequencing |
| title | Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary Infections |
| title_full | Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary Infections |
| title_fullStr | Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary Infections |
| title_full_unstemmed | Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary Infections |
| title_short | Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary Infections |
| title_sort | significance of combining bronchoalveolar lavage fluid with targeted next generation sequencing in the pathogen detection based diagnosis of pulmonary infections |
| topic | bronchoalveolar lavage fluid conventional microbiological tests metagenomic next‐generation sequencing pulmonary infection targeted next‐generation sequencing |
| url | https://doi.org/10.1111/crj.70046 |
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