Comparison of <i>In Vitro</i> Hair Growth Promotion and Anti-Hair Loss Potential of Thai Rice By-Product from <i>Oryza sativa</i> L. cv. Buebang 3 CMU and Sanpatong
The bioactive compounds in herbal extracts may provide effective hair loss treatments with fewer side effects compared to synthetic medicines. This study evaluated the effects of Buebang 3 CMU and Sanpatong rice bran extracts, macerated with dichloromethane or 95% ethanol, on hair growth promotion a...
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MDPI AG
2024-11-01
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| author | Anurak Muangsanguan Warintorn Ruksiriwanich Chaiwat Arjin Sansanee Jamjod Chanakan Prom-u-Thai Pensak Jantrawut Pornchai Rachtanapun Patipan Hnorkaew Apinya Satsook Mathukorn Sainakham Juan Manuel Castagnini Korawan Sringarm |
| author_facet | Anurak Muangsanguan Warintorn Ruksiriwanich Chaiwat Arjin Sansanee Jamjod Chanakan Prom-u-Thai Pensak Jantrawut Pornchai Rachtanapun Patipan Hnorkaew Apinya Satsook Mathukorn Sainakham Juan Manuel Castagnini Korawan Sringarm |
| author_sort | Anurak Muangsanguan |
| collection | DOAJ |
| description | The bioactive compounds in herbal extracts may provide effective hair loss treatments with fewer side effects compared to synthetic medicines. This study evaluated the effects of Buebang 3 CMU and Sanpatong rice bran extracts, macerated with dichloromethane or 95% ethanol, on hair growth promotion and hair loss prevention. Overall, Buebang 3 CMU extracts contained significantly higher levels of bioactive compounds, including <i>γ</i>-oryzanol, tocopherols, and various polyphenols such as phytic acid, ferulic acid, and chlorogenic acid, compared to Sanpatong extracts. Additionally, ethanolic extracts demonstrated greater bioactive content and antioxidant activities than those extracted with dichloromethane. These compounds enhanced the proliferation of human hair follicle dermal papilla cells (HFDPCs) by 124.28 ± 1.08% (<i>p</i> < 0.05) and modulated anti-inflammatory pathways by reducing nitrite production to 3.20 ± 0.36 µM (<i>p</i> < 0.05). Key hair growth signaling pathways, including Wnt/β-catenin (CTNNB1), Sonic Hedgehog (SHH, SMO, GLI1), and vascular endothelial growth factor (VEGF), were activated by approximately 1.5-fold to 2.5-fold compared to minoxidil. Also, in both human prostate cancer (DU-145) and HFDPC cells, the ethanolic Buebang 3 CMU extract (Et-BB3-CMU) suppressed <i>SRD5A1</i>, <i>SRD5A2</i>, and <i>SRD5A3</i> expression—key pathways in hair loss—by 2-fold and 1.5-fold more than minoxidil and finasteride, respectively. These findings suggest that Et-BB3-CMU holds promise for promoting hair growth and preventing hair loss. |
| format | Article |
| id | doaj-art-7506e39cbc1f4c1b946c400354b279c8 |
| institution | OA Journals |
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| spelling | doaj-art-7506e39cbc1f4c1b946c400354b279c82025-08-20T02:14:23ZengMDPI AGPlants2223-77472024-11-011321307910.3390/plants13213079Comparison of <i>In Vitro</i> Hair Growth Promotion and Anti-Hair Loss Potential of Thai Rice By-Product from <i>Oryza sativa</i> L. cv. Buebang 3 CMU and SanpatongAnurak Muangsanguan0Warintorn Ruksiriwanich1Chaiwat Arjin2Sansanee Jamjod3Chanakan Prom-u-Thai4Pensak Jantrawut5Pornchai Rachtanapun6Patipan Hnorkaew7Apinya Satsook8Mathukorn Sainakham9Juan Manuel Castagnini10Korawan Sringarm11Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandCenter of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandOffice of Research Administration, Chiang Mai University, Chiang Mai 50200, ThailandOffice of Research Administration, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandResearch Group in Innovative Technologies for Sustainable Food (ALISOST), Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy, Universitat de València, Avenida Vicent Andrés Estellés s/n, 46100 Burjassot, SpainCenter of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandThe bioactive compounds in herbal extracts may provide effective hair loss treatments with fewer side effects compared to synthetic medicines. This study evaluated the effects of Buebang 3 CMU and Sanpatong rice bran extracts, macerated with dichloromethane or 95% ethanol, on hair growth promotion and hair loss prevention. Overall, Buebang 3 CMU extracts contained significantly higher levels of bioactive compounds, including <i>γ</i>-oryzanol, tocopherols, and various polyphenols such as phytic acid, ferulic acid, and chlorogenic acid, compared to Sanpatong extracts. Additionally, ethanolic extracts demonstrated greater bioactive content and antioxidant activities than those extracted with dichloromethane. These compounds enhanced the proliferation of human hair follicle dermal papilla cells (HFDPCs) by 124.28 ± 1.08% (<i>p</i> < 0.05) and modulated anti-inflammatory pathways by reducing nitrite production to 3.20 ± 0.36 µM (<i>p</i> < 0.05). Key hair growth signaling pathways, including Wnt/β-catenin (CTNNB1), Sonic Hedgehog (SHH, SMO, GLI1), and vascular endothelial growth factor (VEGF), were activated by approximately 1.5-fold to 2.5-fold compared to minoxidil. Also, in both human prostate cancer (DU-145) and HFDPC cells, the ethanolic Buebang 3 CMU extract (Et-BB3-CMU) suppressed <i>SRD5A1</i>, <i>SRD5A2</i>, and <i>SRD5A3</i> expression—key pathways in hair loss—by 2-fold and 1.5-fold more than minoxidil and finasteride, respectively. These findings suggest that Et-BB3-CMU holds promise for promoting hair growth and preventing hair loss.https://www.mdpi.com/2223-7747/13/21/3079rice bran (<i>Oryza sativa</i> L.)Buebang 3 CMUSanpatongandrogenetic alopecia5α-reductase inhibitionhair growth promotion |
| spellingShingle | Anurak Muangsanguan Warintorn Ruksiriwanich Chaiwat Arjin Sansanee Jamjod Chanakan Prom-u-Thai Pensak Jantrawut Pornchai Rachtanapun Patipan Hnorkaew Apinya Satsook Mathukorn Sainakham Juan Manuel Castagnini Korawan Sringarm Comparison of <i>In Vitro</i> Hair Growth Promotion and Anti-Hair Loss Potential of Thai Rice By-Product from <i>Oryza sativa</i> L. cv. Buebang 3 CMU and Sanpatong Plants rice bran (<i>Oryza sativa</i> L.) Buebang 3 CMU Sanpatong androgenetic alopecia 5α-reductase inhibition hair growth promotion |
| title | Comparison of <i>In Vitro</i> Hair Growth Promotion and Anti-Hair Loss Potential of Thai Rice By-Product from <i>Oryza sativa</i> L. cv. Buebang 3 CMU and Sanpatong |
| title_full | Comparison of <i>In Vitro</i> Hair Growth Promotion and Anti-Hair Loss Potential of Thai Rice By-Product from <i>Oryza sativa</i> L. cv. Buebang 3 CMU and Sanpatong |
| title_fullStr | Comparison of <i>In Vitro</i> Hair Growth Promotion and Anti-Hair Loss Potential of Thai Rice By-Product from <i>Oryza sativa</i> L. cv. Buebang 3 CMU and Sanpatong |
| title_full_unstemmed | Comparison of <i>In Vitro</i> Hair Growth Promotion and Anti-Hair Loss Potential of Thai Rice By-Product from <i>Oryza sativa</i> L. cv. Buebang 3 CMU and Sanpatong |
| title_short | Comparison of <i>In Vitro</i> Hair Growth Promotion and Anti-Hair Loss Potential of Thai Rice By-Product from <i>Oryza sativa</i> L. cv. Buebang 3 CMU and Sanpatong |
| title_sort | comparison of i in vitro i hair growth promotion and anti hair loss potential of thai rice by product from i oryza sativa i l cv buebang 3 cmu and sanpatong |
| topic | rice bran (<i>Oryza sativa</i> L.) Buebang 3 CMU Sanpatong androgenetic alopecia 5α-reductase inhibition hair growth promotion |
| url | https://www.mdpi.com/2223-7747/13/21/3079 |
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