A novel cryopreservation solution for adipose tissue based on metformin
Abstract Background Autologous fat grafting (AFG) often needs multiple sessions due to low volume retention. Young adipose tissue demonstrates a more pronounced therapeutic effect; thus, the cryopreservation of adipose tissue of young origin is particularly crucial. This study investigated the prote...
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BMC
2025-01-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-025-04142-7 |
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| author | Yaping Deng Xin Liu Xichao Jian Yan Zhang Yinchi Hou Suyun Hou Fang Qi Shune Xiao Chengliang Deng |
| author_facet | Yaping Deng Xin Liu Xichao Jian Yan Zhang Yinchi Hou Suyun Hou Fang Qi Shune Xiao Chengliang Deng |
| author_sort | Yaping Deng |
| collection | DOAJ |
| description | Abstract Background Autologous fat grafting (AFG) often needs multiple sessions due to low volume retention. Young adipose tissue demonstrates a more pronounced therapeutic effect; thus, the cryopreservation of adipose tissue of young origin is particularly crucial. This study investigated the protective effect of a new cryopreservation solution combining trehalose, glycerol, and metformin on adipose tissue. Methods This study initially examined the effect of various concentrations of metformin (0, 1, 2, 4, and 8 mM) on oxidative damage in adipose tissue to identify the optimal concentration. Subsequently, 1.5 mL of fresh human adipose tissue was subjected to freezing using trehalose + glycerol (TG group), trehalose + glycerol + metformin (TGM group), and the common cryoprotectant dimethyl sulfoxide (DMSO) + fetal bovine serum (FBS) (DF group). Samples were cryopreserved in liquid nitrogen for 2 weeks. After thawing, 1 mL of adipose tissue from each group was transplanted subcutaneously into the backs of nude mice. The cryoprotective effects on adipose tissue viability were evaluated during transplantation one month after transplantation. Results The 2 mM concentration of metformin exhibited the lowest reactive oxygen species (ROS) level (29.20 ± 1.73) compared to other concentrations (P < 0.05). Cell proliferation and migration assays also supported the superior performance of the 2 mM concentration. Apoptotic analyses of stromal vascular fraction (SVF) cells showed the lowest levels in the 2 mM group. Compared to other cryopreservation groups, the adipose tissue in the TGM group closely resembled fresh adipose tissue in terms of gross structure and histological characteristics, with the lowest apoptosis rate of SVF cells. In vivo analysis revealed the highest tissue retention rate in the TGM group, with histological examination indicating robust structural integrity. Conclusion The TGM cryopreservation solution, containing metformin, greatly preserves adipose tissue, reduces apoptosis, and improves tissue retention rates. This solution was non-toxic and safe, making it well-suited for tissue cryopreservation in clinical settings. |
| format | Article |
| id | doaj-art-74f1fae92a02468fa6b61340bf293c7a |
| institution | Kabale University |
| issn | 1757-6512 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj-art-74f1fae92a02468fa6b61340bf293c7a2025-01-26T12:18:13ZengBMCStem Cell Research & Therapy1757-65122025-01-0116111410.1186/s13287-025-04142-7A novel cryopreservation solution for adipose tissue based on metforminYaping Deng0Xin Liu1Xichao Jian2Yan Zhang3Yinchi Hou4Suyun Hou5Fang Qi6Shune Xiao7Chengliang Deng8Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical UniversityDepartment of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical UniversityAbstract Background Autologous fat grafting (AFG) often needs multiple sessions due to low volume retention. Young adipose tissue demonstrates a more pronounced therapeutic effect; thus, the cryopreservation of adipose tissue of young origin is particularly crucial. This study investigated the protective effect of a new cryopreservation solution combining trehalose, glycerol, and metformin on adipose tissue. Methods This study initially examined the effect of various concentrations of metformin (0, 1, 2, 4, and 8 mM) on oxidative damage in adipose tissue to identify the optimal concentration. Subsequently, 1.5 mL of fresh human adipose tissue was subjected to freezing using trehalose + glycerol (TG group), trehalose + glycerol + metformin (TGM group), and the common cryoprotectant dimethyl sulfoxide (DMSO) + fetal bovine serum (FBS) (DF group). Samples were cryopreserved in liquid nitrogen for 2 weeks. After thawing, 1 mL of adipose tissue from each group was transplanted subcutaneously into the backs of nude mice. The cryoprotective effects on adipose tissue viability were evaluated during transplantation one month after transplantation. Results The 2 mM concentration of metformin exhibited the lowest reactive oxygen species (ROS) level (29.20 ± 1.73) compared to other concentrations (P < 0.05). Cell proliferation and migration assays also supported the superior performance of the 2 mM concentration. Apoptotic analyses of stromal vascular fraction (SVF) cells showed the lowest levels in the 2 mM group. Compared to other cryopreservation groups, the adipose tissue in the TGM group closely resembled fresh adipose tissue in terms of gross structure and histological characteristics, with the lowest apoptosis rate of SVF cells. In vivo analysis revealed the highest tissue retention rate in the TGM group, with histological examination indicating robust structural integrity. Conclusion The TGM cryopreservation solution, containing metformin, greatly preserves adipose tissue, reduces apoptosis, and improves tissue retention rates. This solution was non-toxic and safe, making it well-suited for tissue cryopreservation in clinical settings.https://doi.org/10.1186/s13287-025-04142-7Adipose tissueCryopreservationTrehaloseGlycerolMetforminFat transplantation |
| spellingShingle | Yaping Deng Xin Liu Xichao Jian Yan Zhang Yinchi Hou Suyun Hou Fang Qi Shune Xiao Chengliang Deng A novel cryopreservation solution for adipose tissue based on metformin Stem Cell Research & Therapy Adipose tissue Cryopreservation Trehalose Glycerol Metformin Fat transplantation |
| title | A novel cryopreservation solution for adipose tissue based on metformin |
| title_full | A novel cryopreservation solution for adipose tissue based on metformin |
| title_fullStr | A novel cryopreservation solution for adipose tissue based on metformin |
| title_full_unstemmed | A novel cryopreservation solution for adipose tissue based on metformin |
| title_short | A novel cryopreservation solution for adipose tissue based on metformin |
| title_sort | novel cryopreservation solution for adipose tissue based on metformin |
| topic | Adipose tissue Cryopreservation Trehalose Glycerol Metformin Fat transplantation |
| url | https://doi.org/10.1186/s13287-025-04142-7 |
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