CHARACTERISTIC CHANGES IN THE COPY NUMBER OF INTERSPERSED REPEATS IN BONE MARROW CELLS OF MICE TREATED WITH CYCLOPHOSPHAMIDE AND EXOGENOUS HUMAN DNA
Mice were observed to get sick and die upon administration of exogenous DNA in a specific period of time following their pretreatment with the cytostatic cyclophosphamide (CP) (Dolgova et al., 2011). It was established that exogenous DNA reaches internal compartments of bone marrow cells (BMCs) wher...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2014-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/147 |
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| author | E. V. Dolgova A. V. Prokopenko V. P. Nikolin N. A. Popova A. S. Proskurina K. E. Orishchenko E. A. Alyamkina Ya. R. Efremov E. R. Chernykh A. A. Ostanin S. S. Bogachev T. S. Gvozdeva E. M. Malkova O. S. Taranov V. A. Rogachev A. V. Panov S. N. Zagrebelnyi M. A. Shurdov |
| author_facet | E. V. Dolgova A. V. Prokopenko V. P. Nikolin N. A. Popova A. S. Proskurina K. E. Orishchenko E. A. Alyamkina Ya. R. Efremov E. R. Chernykh A. A. Ostanin S. S. Bogachev T. S. Gvozdeva E. M. Malkova O. S. Taranov V. A. Rogachev A. V. Panov S. N. Zagrebelnyi M. A. Shurdov |
| author_sort | E. V. Dolgova |
| collection | DOAJ |
| description | Mice were observed to get sick and die upon administration of exogenous DNA in a specific period of time following their pretreatment with the cytostatic cyclophosphamide (CP) (Dolgova et al., 2011). It was established that exogenous DNA reaches internal compartments of bone marrow cells (BMCs) where it is processed (Dolgova et al., 2012a). Thus, BMCs appear to be the primary targets for the synergic action of these preparations (Dolgova et al., 2012b).In the present study, we show that the copy number for mouse interspersed genomic repeats decreases in the genome of mouse mononuclear cells as a result of interstrand cross-link (ICL) repair after pre-treatment with cytostatic CP. This phenomenon occurs within the time span from 18 to 24 h following CP injection, which corresponds to the final step in the repair of the majority of double-strand breaks (DSBs), as predominant intermediates in ICL repair. Injections of exogenous DNA in CP-pretreated mice preserve the copy number of interspersed repeats at the original level. Our results suggest that the fragments of exogenous DNA participate in ICL-induced DSB repair, thereby compromising the repair process. |
| format | Article |
| id | doaj-art-74a6e7efc58746fcad4288b140992e8f |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-74a6e7efc58746fcad4288b140992e8f2025-02-01T09:57:59ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592014-12-01172246264131CHARACTERISTIC CHANGES IN THE COPY NUMBER OF INTERSPERSED REPEATS IN BONE MARROW CELLS OF MICE TREATED WITH CYCLOPHOSPHAMIDE AND EXOGENOUS HUMAN DNAE. V. Dolgova0A. V. Prokopenko1V. P. Nikolin2N. A. Popova3A. S. Proskurina4K. E. Orishchenko5E. A. Alyamkina6Ya. R. Efremov7E. R. Chernykh8A. A. Ostanin9S. S. Bogachev10T. S. Gvozdeva11E. M. Malkova12O. S. Taranov13V. A. Rogachev14A. V. Panov15S. N. Zagrebelnyi16M. A. Shurdov17Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Clinical Immunology SB RAMS, Novosibirsk, RussiaInstitute of Clinical Immunology SB RAMS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaNovosibirsk State Medical University, Novosibirsk, RussiaResearch Center for Virology and Biotechnology Vector, Koltsovo, RussiaResearch Center for Virology and Biotechnology Vector, Koltsovo, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaWellsStar College of Health and Human Services, Kennesaw State University Kennesaw, Georgia, USANovosibirsk National Research State University, Novosibirsk, RussiaООО Panagen, Gorno-Altaysk, RussiaMice were observed to get sick and die upon administration of exogenous DNA in a specific period of time following their pretreatment with the cytostatic cyclophosphamide (CP) (Dolgova et al., 2011). It was established that exogenous DNA reaches internal compartments of bone marrow cells (BMCs) where it is processed (Dolgova et al., 2012a). Thus, BMCs appear to be the primary targets for the synergic action of these preparations (Dolgova et al., 2012b).In the present study, we show that the copy number for mouse interspersed genomic repeats decreases in the genome of mouse mononuclear cells as a result of interstrand cross-link (ICL) repair after pre-treatment with cytostatic CP. This phenomenon occurs within the time span from 18 to 24 h following CP injection, which corresponds to the final step in the repair of the majority of double-strand breaks (DSBs), as predominant intermediates in ICL repair. Injections of exogenous DNA in CP-pretreated mice preserve the copy number of interspersed repeats at the original level. Our results suggest that the fragments of exogenous DNA participate in ICL-induced DSB repair, thereby compromising the repair process.https://vavilov.elpub.ru/jour/article/view/147cyclophosphamideexogenous dnainterstrand cross-linksshort interspersed repeat (sine)homologous recombination |
| spellingShingle | E. V. Dolgova A. V. Prokopenko V. P. Nikolin N. A. Popova A. S. Proskurina K. E. Orishchenko E. A. Alyamkina Ya. R. Efremov E. R. Chernykh A. A. Ostanin S. S. Bogachev T. S. Gvozdeva E. M. Malkova O. S. Taranov V. A. Rogachev A. V. Panov S. N. Zagrebelnyi M. A. Shurdov CHARACTERISTIC CHANGES IN THE COPY NUMBER OF INTERSPERSED REPEATS IN BONE MARROW CELLS OF MICE TREATED WITH CYCLOPHOSPHAMIDE AND EXOGENOUS HUMAN DNA Вавиловский журнал генетики и селекции cyclophosphamide exogenous dna interstrand cross-links short interspersed repeat (sine) homologous recombination |
| title | CHARACTERISTIC CHANGES IN THE COPY NUMBER OF INTERSPERSED REPEATS IN BONE MARROW CELLS OF MICE TREATED WITH CYCLOPHOSPHAMIDE AND EXOGENOUS HUMAN DNA |
| title_full | CHARACTERISTIC CHANGES IN THE COPY NUMBER OF INTERSPERSED REPEATS IN BONE MARROW CELLS OF MICE TREATED WITH CYCLOPHOSPHAMIDE AND EXOGENOUS HUMAN DNA |
| title_fullStr | CHARACTERISTIC CHANGES IN THE COPY NUMBER OF INTERSPERSED REPEATS IN BONE MARROW CELLS OF MICE TREATED WITH CYCLOPHOSPHAMIDE AND EXOGENOUS HUMAN DNA |
| title_full_unstemmed | CHARACTERISTIC CHANGES IN THE COPY NUMBER OF INTERSPERSED REPEATS IN BONE MARROW CELLS OF MICE TREATED WITH CYCLOPHOSPHAMIDE AND EXOGENOUS HUMAN DNA |
| title_short | CHARACTERISTIC CHANGES IN THE COPY NUMBER OF INTERSPERSED REPEATS IN BONE MARROW CELLS OF MICE TREATED WITH CYCLOPHOSPHAMIDE AND EXOGENOUS HUMAN DNA |
| title_sort | characteristic changes in the copy number of interspersed repeats in bone marrow cells of mice treated with cyclophosphamide and exogenous human dna |
| topic | cyclophosphamide exogenous dna interstrand cross-links short interspersed repeat (sine) homologous recombination |
| url | https://vavilov.elpub.ru/jour/article/view/147 |
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