Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research
3D spheroids are a valuable tool for investigating the interplay between hypoxia and radioresistance. However, standardized methods for visualizing normoxic and hypoxic regions within spheroids and assessing treatment responses are lacking. We developed a straightforward workflow for the analysis of...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Series: | Clinical and Translational Radiation Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405630825000102 |
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| _version_ | 1832582169431638016 |
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| author | Claire Beckers Lazaros Vasilikos Lorena Moor Martin Pruschy |
| author_facet | Claire Beckers Lazaros Vasilikos Lorena Moor Martin Pruschy |
| author_sort | Claire Beckers |
| collection | DOAJ |
| description | 3D spheroids are a valuable tool for investigating the interplay between hypoxia and radioresistance. However, standardized methods for visualizing normoxic and hypoxic regions within spheroids and assessing treatment responses are lacking. We developed a straightforward workflow for the analysis of spheroids based on image processing using a novel custom-written script, and flow cytometry. |
| format | Article |
| id | doaj-art-749f2dc4db274fef934f9449d78b39b5 |
| institution | Kabale University |
| issn | 2405-6308 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Clinical and Translational Radiation Oncology |
| spelling | doaj-art-749f2dc4db274fef934f9449d78b39b52025-01-30T05:14:31ZengElsevierClinical and Translational Radiation Oncology2405-63082025-03-0151100920Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related researchClaire Beckers0Lazaros Vasilikos1Lorena Moor2Martin Pruschy3Laboratory for Applied Radiobiology, Department of Radiation Oncology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandLaboratory for Applied Radiobiology, Department of Radiation Oncology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandLaboratory for Applied Radiobiology, Department of Radiation Oncology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandCorresponding author at: Dept. Radiation Oncology, University Hospital Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland.; Laboratory for Applied Radiobiology, Department of Radiation Oncology, University Hospital Zurich, University of Zurich, Zurich, Switzerland3D spheroids are a valuable tool for investigating the interplay between hypoxia and radioresistance. However, standardized methods for visualizing normoxic and hypoxic regions within spheroids and assessing treatment responses are lacking. We developed a straightforward workflow for the analysis of spheroids based on image processing using a novel custom-written script, and flow cytometry.http://www.sciencedirect.com/science/article/pii/S2405630825000102SpheroidsRadiotherapyHypoxiaFluorescence microscopyFlow cytometry3D cell culture |
| spellingShingle | Claire Beckers Lazaros Vasilikos Lorena Moor Martin Pruschy Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research Clinical and Translational Radiation Oncology Spheroids Radiotherapy Hypoxia Fluorescence microscopy Flow cytometry 3D cell culture |
| title | Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research |
| title_full | Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research |
| title_fullStr | Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research |
| title_full_unstemmed | Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research |
| title_short | Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research |
| title_sort | live cell imaging and analysis of 3d spheroids in hypoxia and radiotherapy related research |
| topic | Spheroids Radiotherapy Hypoxia Fluorescence microscopy Flow cytometry 3D cell culture |
| url | http://www.sciencedirect.com/science/article/pii/S2405630825000102 |
| work_keys_str_mv | AT clairebeckers livecellimagingandanalysisof3dspheroidsinhypoxiaandradiotherapyrelatedresearch AT lazarosvasilikos livecellimagingandanalysisof3dspheroidsinhypoxiaandradiotherapyrelatedresearch AT lorenamoor livecellimagingandanalysisof3dspheroidsinhypoxiaandradiotherapyrelatedresearch AT martinpruschy livecellimagingandanalysisof3dspheroidsinhypoxiaandradiotherapyrelatedresearch |