Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats
Objective. To study the effects of the three methods and three-acupoint technique on DRG gene expression in SNI model rats and to elucidate the molecular mechanism of the three methods and three-acupoint technique on promoting recovery in peripheral nerve injury. Methods. 27 male SD rats were random...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Pain Research and Management |
| Online Access: | http://dx.doi.org/10.1155/2020/7531409 |
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| author | Tao-tao Lv Yan-jun Mo Tian-yuan Yu Shuai Shao Meng-qian Lu Yu-ting Luo Yi Shen Yu-mo Zhang Wong Steven |
| author_facet | Tao-tao Lv Yan-jun Mo Tian-yuan Yu Shuai Shao Meng-qian Lu Yu-ting Luo Yi Shen Yu-mo Zhang Wong Steven |
| author_sort | Tao-tao Lv |
| collection | DOAJ |
| description | Objective. To study the effects of the three methods and three-acupoint technique on DRG gene expression in SNI model rats and to elucidate the molecular mechanism of the three methods and three-acupoint technique on promoting recovery in peripheral nerve injury. Methods. 27 male SD rats were randomly divided into three groups: a Sham group, the SNI group, and the Tuina group. The Tuina group was treated with a tuina manipulation simulator to simulate massage on points, controlling for both quality and quantity. Point-pressing, plucking, and kneading methods were administered quantitatively at Yinmen (BL37), Chengshan (BL57), and Yanglingquan (GB34) points on the affected side once a day, beginning 7 days after modeling. Intervention was applied once a day for 10 days, then 1 day of rest, followed by 10 more days of intervention, totally equaling 20 times of intervention. The effect of the three methods and three-point technique on the recovery of injured rats was evaluated using behavior analysis. RNA sequencing (RNA-Seq) analysis of differentially expressed genes in DRGs of the three groups of rats was also performed. GO and KEGG enrichment was analyzed and verified using real-time PCR. Results. RNA-Seq combined with database information showed that the number of differentially expressed genes in DRG was the largest in the Tuina group compared with the SNI group, totaling 226. GO function is enriched in the positive regulation of cell processes, ion binding, protein binding, neuron, response to pressure, response to metal ions, neuron projection, and other biological processes. GO function is also enriched in the Wnt, IL-17, and MAPK signaling pathways in the KEGG database. PCR results were consistent with those of RNA sequencing, suggesting that the results of transcriptome sequencing were reliable. Conclusion. The three methods and three-acupoint technique can promote the recovery of SNI model rats by altering the gene sequence in DRGs. |
| format | Article |
| id | doaj-art-749785d4a415412bac025e46923286dc |
| institution | Kabale University |
| issn | 1203-6765 1918-1523 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pain Research and Management |
| spelling | doaj-art-749785d4a415412bac025e46923286dc2025-02-03T01:00:09ZengWileyPain Research and Management1203-67651918-15232020-01-01202010.1155/2020/75314097531409Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured RatsTao-tao Lv0Yan-jun Mo1Tian-yuan Yu2Shuai Shao3Meng-qian Lu4Yu-ting Luo5Yi Shen6Yu-mo Zhang7Wong Steven8School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaObjective. To study the effects of the three methods and three-acupoint technique on DRG gene expression in SNI model rats and to elucidate the molecular mechanism of the three methods and three-acupoint technique on promoting recovery in peripheral nerve injury. Methods. 27 male SD rats were randomly divided into three groups: a Sham group, the SNI group, and the Tuina group. The Tuina group was treated with a tuina manipulation simulator to simulate massage on points, controlling for both quality and quantity. Point-pressing, plucking, and kneading methods were administered quantitatively at Yinmen (BL37), Chengshan (BL57), and Yanglingquan (GB34) points on the affected side once a day, beginning 7 days after modeling. Intervention was applied once a day for 10 days, then 1 day of rest, followed by 10 more days of intervention, totally equaling 20 times of intervention. The effect of the three methods and three-point technique on the recovery of injured rats was evaluated using behavior analysis. RNA sequencing (RNA-Seq) analysis of differentially expressed genes in DRGs of the three groups of rats was also performed. GO and KEGG enrichment was analyzed and verified using real-time PCR. Results. RNA-Seq combined with database information showed that the number of differentially expressed genes in DRG was the largest in the Tuina group compared with the SNI group, totaling 226. GO function is enriched in the positive regulation of cell processes, ion binding, protein binding, neuron, response to pressure, response to metal ions, neuron projection, and other biological processes. GO function is also enriched in the Wnt, IL-17, and MAPK signaling pathways in the KEGG database. PCR results were consistent with those of RNA sequencing, suggesting that the results of transcriptome sequencing were reliable. Conclusion. The three methods and three-acupoint technique can promote the recovery of SNI model rats by altering the gene sequence in DRGs.http://dx.doi.org/10.1155/2020/7531409 |
| spellingShingle | Tao-tao Lv Yan-jun Mo Tian-yuan Yu Shuai Shao Meng-qian Lu Yu-ting Luo Yi Shen Yu-mo Zhang Wong Steven Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats Pain Research and Management |
| title | Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats |
| title_full | Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats |
| title_fullStr | Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats |
| title_full_unstemmed | Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats |
| title_short | Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats |
| title_sort | using rna seq to explore the repair mechanism of the three methods and three acupoint technique on drgs in sciatic nerve injured rats |
| url | http://dx.doi.org/10.1155/2020/7531409 |
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